CAZyme Information: RQM14167.1

Basic Information

• Species: Peronospora effusa
• Lineage: Oomycota; NA; ; Peronosporaceae; Peronospora; Peronospora effusa
• CAZyme ID: RQM14167.1
• CAZy Family: GH5|GH5
• CAZyme Description: Endo-1,3(4)-beta-glucanase [Source:UniProtKB/TrEMBL;Acc:A0A3R7Y7V9]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
719		80697.48	4.7568

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PeffusaR13	8603	N/A	0	8603

• Gene Location: location=QKXF01000217:50037-52212(-)

Full Sequence      

MEGPLGSPLDGYFSPFSLDEPSEKLFKRNGSLETIAPIINVAKEALNQPIPTNKWWGNLI
HVTATNLSENYPVWAQPYAMMLPKEQPYGLMAYYPFTYREIAPEVDGIVKYYEHDIHNDL
TLSAQEFNVTKPVYEIYSWDDMGINLRICDPSSNKCMDSALLNGMAFISGKYDGLTPRID
TEFNITSVDNSTPGKYVIHLNNTQKWVLYASESISFRVEESVMFSVNASGSSLVADGGYH
GTIRLAVLPEGAEDTVYDKYAPCMVRGGTVSMESRTAYSINWDVEGSTCESVGLLHFALP
HQVASLTGSPTTASTPGAIALHSSTRGLMVAQVSNKWCFVEPESEIEIDFWPARRPTPMV
VEEFDMLRTLKDDITANWSMNASSWYFNGKNFQKYASLCLMAADSSVVGPDTTLLAFCLE
KLEKLIEPLLNNTLIPPLVYETLYRGIVSRAIFDTGELYVDFGNGIYNDHHYHYGYYVTS
AAIIKYLNPNWSRMPELEALVWLMVRDVANPSRYDCFFPRFRHFSWFNGLSYSRGVTLLN
EGKDEESTSEDINFYYGMTLWGKVTGRKAVEDLGSLMLRLNAHAIRTYVLLTSDSTIHPP
EIVANHVTGIFFDNKVFYTTWFLDKVYAIHGIQMIPVIYRTPTFVTQEWTDILSKDEIVT
TPNSNNTWLSLLLVNAAILDPMNSLRKLQNASMDDGLSLSWALYNAATRGSGPGVGTNA

Enzyme Prediction     

No EC number prediction in RQM14167.1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH81	41	704	8.7e-160	0.9807073954983923

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
407548	Glyco_hydro81C	1.67e-119	370	703	18	349	Glycosyl hydrolase family 81 C-terminal domain. Family of eukaryotic beta-1,3-glucanases. Within the Aspergillus fumigatus protein, two perfectly conserved Glu residues (E550 or E554) have been proposed as putative nucleophiles of the active site of the Engl1 endoglucanase, while the proton donor would be D475. The endo-beta-1,3-glucanase activity is essential for efficient spore release. This entry represents the helical C-terminal domain.
227785	Acf2	1.05e-115	49	711	89	758	Endoglucanase Acf2 [Carbohydrate transport and metabolism].
397619	Glyco_hydro_81	6.39e-54	44	352	9	318	Glycosyl hydrolase family 81. Family of eukaryotic beta-1,3-glucanases. Within the Aspergillus fumigatus protein ENGL1, two perfectly conserved Glu residues (E550 or E554) have been proposed as putative nucleophiles of the active site of the Engl1 endoglucanase, while the proton donor would be D475. The endo-beta-1,3-glucanase activity is essential for efficient spore release.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UIZ26131.1|GH81	0.0	1	719	1	724
UIZ26137.1|GH81	0.0	4	719	114	834
UIZ26602.1|GH81	0.0	4	719	114	834
UIZ26438.1|GH81	6.14e-309	12	710	44	741
UIZ26440.1|GH81	6.14e-309	12	710	44	741

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4K3A_A	2.67e-94	50	709	41	718	The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei],4K3A_B The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei]
5XBZ_A	4.54e-94	50	709	66	743	Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminaripentaose [Rhizomucor miehei],5XBZ_B Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminaripentaose [Rhizomucor miehei],5XC2_A Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei],5XC2_B Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei],5XC2_C Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei],5XC2_D Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei]
4K35_A	1.11e-89	50	709	41	718	The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei],4K35_B The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei]
6FOP_A	9.58e-24	468	681	414	627	Chain A, Glycoside hydrolase family 81 [Acetivibrio thermocellus ATCC 27405]
5V1W_A	9.29e-19	468	695	439	664	Chain A, Glycoside Hydrolase [Halalkalibacterium halodurans C-125]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q09850|ENG2_SCHPO	4.00e-87	49	713	34	706	Ascus wall endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng2 PE=1 SV=1
sp|Q9UT45|ENG1_SCHPO	1.48e-80	41	707	73	735	Primary septum endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng1 PE=3 SV=1
sp|D4AZ24|ENG1_ARTBC	4.19e-78	49	707	225	900	Probable endo-1,3(4)-beta-glucanase ARB_01444 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_01444 PE=1 SV=1
sp|Q5AIR7|ENG1_CANAL	4.50e-75	48	707	459	1139	Endo-1,3(4)-beta-glucanase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=ENG1 PE=1 SV=1
sp|Q12168|ENG2_YEAST	2.20e-72	49	707	110	777	Endo-1,3(4)-beta-glucanase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ACF2 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000047	0.000000

TMHMM  Annotations     

There is no transmembrane helices in RQM14167.1.