CAZyme Information: RQM13838.1

Basic Information

• Species: Peronospora effusa
• Lineage: Oomycota; NA; ; Peronosporaceae; Peronospora; Peronospora effusa
• CAZyme ID: RQM13838.1
• CAZy Family: GH54
• CAZyme Description: unspecified product

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
489	QKXF01000232|CGC1	54647.70	5.2475

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PeffusaR13	8603	N/A	0	8603

• Gene Location: location=QKXF01000232:61079-63091(+)

Full Sequence      

MQEEPPGSSCGRWLARRSMSQLDNGDSRGRDSSSLSSVSLLPASTTDARVVAISCMSDDN
EHEHEEVTDADDASLNVYKFDVTQYKDGTFRFVPACARDLIKYTGRLLYHGAQCKRKTKI
NTVQFDWPAVTFQITVQGTSCVAIRLKGDGNYFNVFVNDELRCILRASLNATCCDVATDL
DTSLEYAIRICKRTEPQMRGAMSTFKVCTFYGFIVEENAEVFPVDQSEPQFIRKMEFIGD
SDTCGFGNEGKASSAKNLFGMKGRMENVYNGYACVTARMFDAEEHVLAWSGKGVHSNSAD
WGPNMPILWKNTLASRPGDWDMSSWIPDVVVINLGINDLSPPASAETDIITGYAAFLLEV
RSYRPDAHIFCVVYDDGCISSEDLETNRKFVSLQLQEIVKVAISKVSKHDDKMHYTFIKV
DEGLEKEDYASMMHYAVSGHIKIAKVLAEEIALRTGWNVEHEPKTMPYPQAKDKILTPRD
HSKTSCNVS

Enzyme Prediction     

No EC number prediction in RQM13838.1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
CE2	234	451	7.1e-55	0.9952153110047847

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
238869	Endoglucanase_E_like	5.59e-32	234	451	1	168	Endoglucanase E-like members of the SGNH hydrolase family; Endoglucanase E catalyzes the endohydrolysis of 1,4-beta-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
407839	CE2_N	8.12e-14	122	223	12	106	Carbohydrate esterase 2 N-terminal. This is the N-terminal beta-sheet domain with jelly roll topology found in CE2 acetyl-esterase from the bacterium Clostridium thermocellum. This enzyme displays dual activities, it catalyses the deacetylation of plant polysaccharides and also potentiates the activity of its appended cellulase catalytic module through its noncatalytic cellulose binding function. This N-terminal jelly-roll domain appears to extend the substrate/cellulose binding cleft of the catalytic domain in C.thermocellum.
404371	Lipase_GDSL_2	5.42e-06	237	372	1	106	GDSL-like Lipase/Acylhydrolase family. This family of presumed lipases and related enzymes are similar to pfam00657.
238871	XynB_like	1.85e-04	327	370	41	83	SGNH_hydrolase subfamily, similar to Ruminococcus flavefaciens XynB. Most likely a secreted hydrolase with xylanase activity. SGNH hydrolases are a diverse family of lipases and esterases. The tertiary fold of the enzyme is substantially different from that of the alpha/beta hydrolase family and unique among all known hydrolases; its active site closely resembles the Ser-His-Asp(Glu) triad found in other serine hydrolases.
238141	SGNH_hydrolase	2.20e-04	237	449	3	186	SGNH_hydrolase, or GDSL_hydrolase, is a diverse family of lipases and esterases. The tertiary fold of the enzyme is substantially different from that of the alpha/beta hydrolase family and unique among all known hydrolases; its active site closely resembles the typical Ser-His-Asp(Glu) triad from other serine hydrolases, but may lack the carboxlic acid.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UIZ26481.1|CE2	0.0	1	489	1	522
CCA14218.1|CE2	1.79e-129	55	488	25	468
QNU68524.1|CE2	1.81e-35	124	457	131	440
ACL76504.1|CE2	2.34e-35	124	457	127	436
AEY66733.1|CE2	3.21e-35	124	457	127	436

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2WAO_A	4.10e-29	127	461	29	336	Chain A, ENDOGLUCANASE E [Acetivibrio thermocellus]
2WAB_A	1.06e-28	127	461	29	336	Chain A, ENDOGLUCANASE E [Acetivibrio thermocellus]
2W9X_A	2.20e-15	125	377	50	297	The active site of a carbohydrate esterase displays divergent catalytic and non-catalytic binding functions [Cellvibrio japonicus],2W9X_B The active site of a carbohydrate esterase displays divergent catalytic and non-catalytic binding functions [Cellvibrio japonicus]
4XVH_A	7.23e-14	122	457	12	329	Crystal structure of a Corynascus thermopiles (Myceliophthora fergusii) carbohydrate esterase family 2 (CE2) enzyme plus carbohydrate binding domain (CBD) [Chaetomium]
3U37_A	2.18e-12	226	457	159	406	An Acetyl Xylan Esterase (Est2A) from the Rumen Bacterium Butyrivibrio proteoclasticus. [Butyrivibrio proteoclasticus B316],3U37_B An Acetyl Xylan Esterase (Est2A) from the Rumen Bacterium Butyrivibrio proteoclasticus. [Butyrivibrio proteoclasticus B316],3U37_C An Acetyl Xylan Esterase (Est2A) from the Rumen Bacterium Butyrivibrio proteoclasticus. [Butyrivibrio proteoclasticus B316],3U37_D An Acetyl Xylan Esterase (Est2A) from the Rumen Bacterium Butyrivibrio proteoclasticus. [Butyrivibrio proteoclasticus B316],3U37_E An Acetyl Xylan Esterase (Est2A) from the Rumen Bacterium Butyrivibrio proteoclasticus. [Butyrivibrio proteoclasticus B316],3U37_F An Acetyl Xylan Esterase (Est2A) from the Rumen Bacterium Butyrivibrio proteoclasticus. [Butyrivibrio proteoclasticus B316],3U37_G An Acetyl Xylan Esterase (Est2A) from the Rumen Bacterium Butyrivibrio proteoclasticus. [Butyrivibrio proteoclasticus B316],3U37_H An Acetyl Xylan Esterase (Est2A) from the Rumen Bacterium Butyrivibrio proteoclasticus. [Butyrivibrio proteoclasticus B316]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P10477|CELE_ACET2	9.32e-27	127	457	510	814	Cellulase/esterase CelE OS=Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=celE PE=1 SV=2
sp|B3PDE5|CE2B_CELJU	1.08e-14	125	377	50	297	Acetylxylan esterase / glucomannan deacetylase OS=Cellvibrio japonicus (strain Ueda107) OX=498211 GN=ce2C PE=1 SV=1
sp|B3PIB0|CE2A_CELJU	2.05e-11	113	383	40	298	Acetylxylan esterase / glucomannan deacetylase OS=Cellvibrio japonicus (strain Ueda107) OX=498211 GN=axe2C PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000074	0.000000

TMHMM  Annotations     

There is no transmembrane helices in RQM13838.1.