CAZyme Information: RQM12264.1

Basic Information

• Species: Peronospora effusa
• Lineage: Oomycota; NA; ; Peronosporaceae; Peronospora; Peronospora effusa
• CAZyme ID: RQM12264.1
• CAZy Family: GH17
• CAZyme Description: unspecified product

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1666		187918.15	5.0292

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PeffusaR13	8603	N/A	0	8603

• Gene Location: location=QKXF01000344:19182-24333(-)

Full Sequence      

MRLTTLLNAGLAALVATVQLSLTSARGVHVNLTASWPSSPFFPLLETSEFLAEENPLYFW
KFVDELSTRTSYVNDIRNDVDALGALAVFIAESIAPGSKNILELMLATRAYSVKVEMFRQ
LALDSKARLCGADVDTWAVFYQEPQCVESVACSVNELEAILHGNKQQETHEVCVAAGSND
VELQVDHKYPHIASSTVKTPMSVILYGLVGTEKFYAFHTNLAKKAKKDEIQYMVRHYPRD
SPLDTLLQGYGVALDIKNMEYKTFDDSKKTGEENAVGDDKGDEEENEEEEDIEDEEVGGF
LFKPLMARHKAIADDLKQFYDILVKKADGDQEQELKAWHLKDVGVSAARAIVDAKNPLKM
LETLSQDFPVQVKKLVFSRKIISPELRKEISVTRMQAANRLLTNKFIMNGIAVDPMERSF
NVFDFMKTLKEEWSVAKQLGRLALNQTELEDMLTHVRETNQEQPVVRIHMRGSMNGTTPL
YLNNIETDSNYADWPPDVNLLRRPAWNLIFVRKNMYECILVLDPLTGAGRAALTHIAFMR
MRGAPVQWALLISSKELMASKTSDDRRALLEKYKSLKDYEKATPWHFAKLLMLAQSKDTA
SAENKTPESGNEKTGEAEVEADHTVSTRKIASAFLERVGGIEQAEVLVENLLEAYTGAAA
DIGTFEENEEEALAYLRSDQFDNELLAMTEFIHMKHVPLGSFVFNGVIQKDLDIQRSMMN
SFGRDQPLYVDMAHRGMLDNEMDLIEELLIAQNAHPAFLSIFESPESRHPGDIEVESHLF
ADDVDGRLEAAAWPAITYLHAPGSRIVPKMQTVIFPADLSDLRDANYAYRVVKAVLEDSK
QSLRVGVVPQLKGASKTNDMSNDVGELVAAILVAVGNSDNEAHIAFILEALNCVIMQKTA
DAMKEKLIGIWKTTKNKDSSKDLVYDKVLALLENKTGGWLTTTQRDILTQFNTLLRSRFP
PAFEEESEEGSSKIALPHLFINGRQVSLPSQSFSDGDMATIAKFDLQYRTQPVAKALLKR
SVIQTTEDADKLSFDIMKTTGIVDKYVKTERESRLGVEENSLNSVRLPGDPSLQVAAYVD
PLSEAAQVISSMLRTFHSQLNATIELVLVPADEYTEFPLHRFYRYLFEKRPSLAATSVEF
RKLPVQPILTMKIDTPEAWNVQSLHAGDDLDNLRVDPESLTEVKSTTRAVFRLESLLVYG
QCRDATLQMYSPPNGLQLALERQVGAQLLHRDTLVMKNLGYFQLQATPGVWSLHLARGRA
ADLFDIIDPETDLPLKTHPIIVYDFGSHIFQLLVRKKDGMEHEELLQSVEDAATSSKSKS
DADAKSTGKDDGALRSYWSSMLQMMGKHDNEIESTPQAVADKTDETDLTNKGNTMQQKQR
TGETIHVFSVASGYLYERFVKIMMLSVLKRTNNPVTFWVLENFLSPDFKASIPALREQLG
MDIRLITYKWPNWLRQQTEKQRIIWGYKILFLDVLFPLGVQKIIYVDADQVVRADLKELL
ELDMEGKPYGYTPFCDSRNVGFQFWRQGYWKDHLRGKPYHISALYVVDLAVFRQMAAGDM
LRATYSQLSADPNSLANLDQDLPNFVQHQLPIFSLPQEWLWCESWCSDETKATAKTIDLC
NNPKHKEPKLDMAKRVIAGELFKESWIELDQEIKDAETKYTAQAAA

Enzyme Prediction     

EC	2.4.1.-:29

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT24	1385	1630	3.3e-122	0.9959677419354839

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
408203	Glyco_transf_24	0.0	1385	1655	1	267	Glucosyltransferase 24. This is the catalytic domain found in UDP-glucose:glycoprotein glucosyltransferase (UGGT). This domain belongs to glucosyltransferase 24 family (GT24) A-type domain. The GT domain displays the expected glycosyltransferase type A (GT-A) fold.
133054	GT8_HUGT1_C_like	1.86e-161	1385	1630	1	248	The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity.
132996	Glyco_transf_8	4.12e-59	1385	1625	1	243	Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+.
408199	Thioredoxin_12	1.38e-40	38	242	1	182	Thioredoxin-like domain. This is one of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).
399437	UDP-g_GGTase	5.71e-25	1200	1307	2	109	UDP-glucose:Glycoprotein Glucosyltransferase. UDP-g_GGTase is an important, central component of the QC system in the ER for checking that glycoproteins are folded correctly. This QC prevents incorrectly folded glycoproteins from leaving the ER.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UIZ20865.1|GT24	0.0	1	1666	1	1666
CCA14500.1|GT24	0.0	25	1662	35	1667
AIG56125.1|GT24	0.0	11	1662	11	1507
AIG55512.1|GT24	0.0	24	1660	22	1515
AIZ94008.1|GT24	1.28e-202	26	1654	34	1595

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MU1_A	8.93e-148	203	1655	163	1446	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5MZO_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TRF_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5NV4_A	8.93e-148	203	1655	163	1446	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TRT_A	3.18e-147	203	1655	163	1446	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS2_A	1.54e-136	1077	1655	660	1212	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_C Chain C, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_D Chain D, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS8_A	1.71e-121	203	1592	156	1382	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS8_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q0WL80|UGGG_ARATH	3.68e-201	26	1662	31	1570	UDP-glucose:glycoprotein glucosyltransferase OS=Arabidopsis thaliana OX=3702 GN=UGGT PE=1 SV=1
sp|Q6P5E4|UGGG1_MOUSE	1.36e-196	13	1660	32	1527	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Mus musculus OX=10090 GN=Uggt1 PE=1 SV=4
sp|Q9JLA3|UGGG1_RAT	3.68e-195	13	1660	32	1527	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Rattus norvegicus OX=10116 GN=Uggt1 PE=1 SV=2
sp|Q9NYU2|UGGG1_HUMAN	5.62e-192	25	1660	44	1527	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Homo sapiens OX=9606 GN=UGGT1 PE=1 SV=3
sp|Q09332|UGGG_DROME	6.60e-187	32	1654	34	1505	UDP-glucose:glycoprotein glucosyltransferase OS=Drosophila melanogaster OX=7227 GN=Uggt PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000259	0.999714	CS pos: 25-26. Pr: 0.9677

TMHMM  Annotations     

There is no transmembrane helices in RQM12264.1.