CAZyme Information: RQM09706.1

Basic Information

• Species: Peronospora effusa
• Lineage: Oomycota; NA; ; Peronosporaceae; Peronospora; Peronospora effusa
• CAZyme ID: RQM09706.1
• CAZy Family: AA17|AA17|AA17
• CAZyme Description: unspecified product

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
730	QKXF01000674|CGC1	80671.52	6.3642

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PeffusaR13	8603	N/A	0	8603

• Gene Location: location=QKXF01000674:25200-27465(-)

Full Sequence      

MRFTAFVTLVTSLSMATVRAAKDFTGATHPTKSGIEIWVDPATPRDRQTYVSSRGRKWDL
VVSDEFNTKNRSFRPGDDHMWTSLDKPDGVNGALEIYSHNMTSTKCDDDGTCYFYIECQD
EVNVIKSWNKLCFQGGMLEVRAQLPGAVSKASKNPDLALGESGEVKTTKFYPTWPGIWMM
GNLGRAIFSASTNRMWPFSYDKCEPDVFDPKNQRINACNDNPGYGLNPNQGRGAPEIDIL
EGSGLVISSSLQIAPGMPKDFRLFSPNDDGANQACVYKYNCITPGANMIEVPTSYYKKQR
GHKSWYQGLRYGANNHCKPNAKAKQDYDTVAASVKAGITKNTCSVDTCPASVDVNGDLGF
MDDSKTSHWGINSNGTCHSVMNSYMGDYLCDPDNTNLKCTKPRDPTSPKFNSMSEFNYQI
DAISSNWPVHLGAYLGYLKYQLEWVTGANGYVRWMLDGHPLFEVTTEAFSNVPQSSKKEN
PQKVMLEEPMYLIFNVALSKSWGTTPPNPGKECRGNGSDPVANVICDSFPMYMKIDYIRL
YQDRGDDLDKDNYMQIGCDPSSHPTKKWIDGHIDEFVDDDNPLMEVSGKAFCGKDDDCTI
AGSVGRVALKTGTCVDGRCQCTYSTSWGGPRCTTALAGSTSTSASSYSQRAFGPPASLSL
SIGVISVFLSILSVCLSMKSIKEEDAALTKMNAYTKMANNSDDGNVRNCSMNRNHSMNQR
PKDNYSQNFV

Enzyme Prediction     

EC	-	-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	64	255	1e-57	0.5828220858895705

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397841	SKN1	3.40e-59	24	574	65	474	Beta-glucan synthesis-associated protein (SKN1). This family consists of the beta-glucan synthesis-associated proteins KRE6 and SKN1. Beta1,6-Glucan is a key component of the yeast cell wall, interconnecting cell wall proteins, beta1,3-glucan, and chitin. It has been postulated that the synthesis of beta1,6-glucan begins in the endoplasmic reticulum with the formation of protein-bound primer structures and that these primer structures are extended in the Golgi complex by two putative glucosyltransferases that are functionally redundant, Kre6 and Skn1. This is followed by maturation steps at the cell surface and by coupling to other cell wall macromolecules.
185689	GH16_fungal_KRE6_glucanase	2.11e-48	60	542	1	295	Saccharomyces cerevisiae KRE6 and related glucanses, member of glycosyl hydrolase family 16. KRE6 is a Saccharomyces cerevisiae glucanase that participates in the synthesis of beta-1,6-glucan, a major structural component of the cell wall. It is a golgi membrane protein required for normal beta-1,6-glucan levels in the cell wall. KRE6 is closely realted to laminarinase, a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
185693	GH16_laminarinase_like	2.87e-09	440	541	163	235	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
225182	BglS	3.87e-08	39	311	30	265	Beta-glucanase, GH16 family [Carbohydrate transport and metabolism].
185683	Glyco_hydrolase_16	9.55e-05	440	541	147	210	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UIZ28149.1|GH16	0.0	1	730	1	754
UIZ28152.1|GH16	0.0	26	730	15	739
UIZ27810.1|GH16	0.0	29	702	65	757
UIZ28151.1|GH16	0.0	5	730	13	752
UIZ28150.1|GH16	5.38e-297	62	730	1	688

PDB Hits     

RQM09706.1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P33336|SKN1_YEAST	4.03e-34	39	591	359	754	Beta-glucan synthesis-associated protein SKN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SKN1 PE=1 SV=1
sp|P32486|KRE6_YEAST	5.80e-34	39	591	307	702	Beta-glucan synthesis-associated protein KRE6 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=KRE6 PE=1 SV=2
sp|P87024|SKN1_CANAX	1.44e-31	39	591	336	729	Beta-glucan synthesis-associated protein SKN1 OS=Candida albicans OX=5476 GN=SKN1 PE=2 SV=1
sp|P87023|KRE6_CANAX	2.41e-29	29	591	321	732	Beta-glucan synthesis-associated protein KRE6 OS=Candida albicans OX=5476 GN=KRE6 PE=2 SV=1
sp|O13941|YEPB_SCHPO	2.66e-29	39	588	219	615	Uncharacterized beta-glucan synthesis-associated protein C23H3.11c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC23H3.11c PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000281	0.999692	CS pos: 20-21. Pr: 0.9738

TMHMM  Annotations     

There is no transmembrane helices in RQM09706.1.