CAZyme Information: RL4_JR_09008-RA-p1

Basic Information

• Species: Raffaelea lauricola
• Lineage: Ascomycota; Sordariomycetes; ; Ophiostomataceae; Raffaelea; Raffaelea lauricola
• CAZyme ID: RL4_JR_09008-RA-p1
• CAZy Family: GT17
• CAZyme Description: unspecified product

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
376	JACBXF010000053|CGC2	41148.74	4.5678

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_RlauricolaRL4	10461	N/A	146	10315

• Gene Location: location=JACBXF010000053:181742-182872(-)

Full Sequence      

MPSKRIKLWLGLLWLAGVRGEGSSSDYPLTTDSNCTCFQTNSTTANFFAHHSFFDFRSMA
EYAATPAVLNGSTANAEATATNAYFQSDAFTSMWALQSWDNGQLMQLNNSDVSGSDGTLL
MVNSPNNIYIERDTSAGAGANATYMVMRTVRLADRLQTAAEMESTSSGFRYLSVRMQART
VGGTGAITAMFTYRAGTGDTVEKVQEADLEIRTRDPATQVQFTNQPSWDADGEIAQATRN
VSMPDSGLWSDWATYRMDWTPGSTSWYVNGDLASTITFQAPRDPSQVVFNSWSDGGTWSG
NMSYNASAFLQIRWIEMVFNNTDKTYKSSARHCSNLCSIDETSVTGTPVLLASIGGRLQP
PWLLLFTTCVALGFAL

Enzyme Prediction     

No EC number prediction in RL4_JR_09008-RA-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	92	317	5e-84	0.9907407407407407

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
185683	Glyco_hydrolase_16	2.09e-47	88	318	7	210	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.
185684	GH16_lichenase	5.16e-10	158	315	56	208	lichenase, member of glycosyl hydrolase family 16. Lichenase, also known as 1,3-1,4-beta-glucanase, is a member of glycosyl hydrolase family 16, that specifically cleaves 1,4-beta-D-glucosidic bonds in mixed-linked beta glucans that also contain 1,3-beta-D-glucosidic linkages. Natural substrates of beta-glucanase are beta-glucans from grain endosperm cell walls or lichenan from the Islandic moss, Cetraria islandica. This protein is found not only in bacteria but also in anaerobic fungi. This domain includes two seven-stranded antiparallel beta-sheets that are adjacent to one another forming a compact, jellyroll beta-sandwich structure.
225182	BglS	1.44e-07	164	309	111	255	Beta-glucanase, GH16 family [Carbohydrate transport and metabolism].
185692	GH16_fungal_CRH1_transglycosylase	3.46e-05	169	300	47	180	glycosylphosphatidylinositol-glucanosyltransferase. Group of fungal GH16 members related to Saccharomyces cerevisiae Crh1p. Chr1p and Crh2p are transglycosylases that are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan, an important step in the assembly of new cell wall. Both have been shown to be glycosylphosphatidylinositol (GPI)-anchored. A third homologous protein, Crr1p, functions in the formation of the spore wall. They belongs to the family 16 of glycosyl hydrolases that includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.
185693	GH16_laminarinase_like	6.20e-05	227	315	135	232	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AEO63760.1|CBM1|GH16_22	2.27e-127	27	360	27	351
AEO55749.1|GH16_22	4.36e-120	28	369	35	404
AEO57601.1|GH16_22	2.29e-100	17	351	28	357
AEO69403.1|GH16_22	2.69e-96	20	355	16	348
QLI63560.1|GH16_22	3.24e-95	29	346	23	350

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1GBG_A	2.27e-10	185	315	86	209	Bacillus Licheniformis Beta-Glucanase [Bacillus licheniformis]
3O5S_A	1.03e-09	168	315	95	233	Crystal Structure of the endo-beta-1,3-1,4 glucanase from Bacillus subtilis (strain 168) [Bacillus subtilis]
1AJK_A	1.88e-09	184	326	2	137	Circularly Permuted (1-3,1-4)-Beta-D-Glucan 4- Glucanohydrolase Cpa16m-84 [Paenibacillus macerans],1AJK_B Circularly Permuted (1-3,1-4)-Beta-D-Glucan 4- Glucanohydrolase Cpa16m-84 [Paenibacillus macerans]
1CPM_A	1.88e-09	184	326	27	162	NATIVE-LIKE IN VIVO FOLDING OF A CIRCULARLY PERMUTED JELLYROLL PROTEIN SHOWN BY CRYSTAL STRUCTURE ANALYSIS [Paenibacillus macerans]
1CPN_A	7.86e-09	184	322	27	158	NATIVE-LIKE IN VIVO FOLDING OF A CIRCULARLY PERMUTED JELLYROLL PROTEIN SHOWN BY CRYSTAL STRUCTURE ANALYSIS [Paenibacillus macerans]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P27051|GUB_BACLI	4.15e-09	185	315	115	238	Beta-glucanase OS=Bacillus licheniformis OX=1402 GN=bg1 PE=1 SV=1
sp|P04957|GUB_BACSU	5.53e-09	168	315	99	237	Beta-glucanase OS=Bacillus subtilis (strain 168) OX=224308 GN=bglS PE=1 SV=2
sp|P07980|GUB_BACAM	1.31e-08	168	315	96	234	Beta-glucanase OS=Bacillus amyloliquefaciens OX=1390 GN=bglA PE=3 SV=1
sp|P45797|GUB_PAEPO	2.36e-08	184	320	109	238	Beta-glucanase OS=Paenibacillus polymyxa OX=1406 GN=gluB PE=3 SV=1
sp|P23904|GUB_PAEMA	7.70e-08	184	320	108	237	Beta-glucanase OS=Paenibacillus macerans OX=44252 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000213	0.999745	CS pos: 20-21. Pr: 0.9821

TMHMM  Annotations     

There is no transmembrane helices in RL4_JR_09008-RA-p1.