CAZyme Information: RL4_JR_08590-RA-p1

Basic Information

• Species: Raffaelea lauricola
• Lineage: Ascomycota; Sordariomycetes; ; Ophiostomataceae; Raffaelea; Raffaelea lauricola
• CAZyme ID: RL4_JR_08590-RA-p1
• CAZy Family: GH92
• CAZyme Description: unspecified product

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1021	JACBXF010000035|CGC2	109130.37	4.6385

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_RlauricolaRL4	10461	N/A	146	10315

• Gene Location: location=JACBXF010000035:413805-417327(-)

Full Sequence      

MVALWPLLAAVGALCTTAVTAAANLTDRVTWDRFSLKVDGTRVFIQSAEFHYQRLPVPEL
WPDVFQKLKANGFNTLSIYFFWSYHSAAPGVFDFETPGKDVQRLFDAAKEAGLWVIARAG
PYCNAETNGGSLALWGSDGSMGKLRTSNETYHQAWLPWISAIGKLIAKNQITNGGPVILN
QIENELQETVYKADNTLVVYMKQIEAAFEEAGVVVPTTSNEKNMRSISWSSDYQNVGGAV
NVYGLDSYPGSGLCSNAVTGFNVLRTYYQWFLKSSPTQPWLLAEFQGGWFSAWGSATFYD
ECTAMHEASFADVYYKNNIGQRATLQSIYMTFGGTNWGHSAAPVVYTSYDYTQTRQQTTK
LFQTKLVNLFSVTSPDLLKTEMVGNGTGFHVSSDFFTWVLSNPDTNATFTVLQQADAKST
ANVSGAVTLDTSAGSLEVPDVALAGRQSKILATDYVLGQAAYSGSTNSNTLLFSSADVAT
SGTFGDEDVLVLYLTEGQTGVFAFRTNENVTFSVYGNSTVVSETTVGGHQAFSYVQGASA
TAVRFSNGVLVYLLDQATAWRFWAPQRGTVTAPQAGYVDRVFVLGPYLVRAASVTDDGVV
QLTGDSDVATAIEVFAGTDAQSLEWNGRRVQVQRTAYGALQGTIPGGADYADALTLPPLD
GNTTWYVADSLPEAQADYDDSRWMVCNKTTTLSPVAPETLPVLFSSDYGFYAGHKVYRGR
FNSSGGVGGVPTAVNITASGGQAFGWTAWLNGAWLGGSGGVAGVSTTSAELAISAGLLAQ
NSSDNVLTVLVDYHGHDETSTGHGVENPRGLLGAALKYGANKTQGPDAGFASWRITGNAG
GNRNIDPVRGPLNEGGLYGERLGWHLPGFDPAQDPAFRTGSGSTPADGLSGAGVRFYVTH
FALGGSAPADGAQVEVPLGLLLGAANVSAEARVQLWINGYQYGKYVPHLGPQTRFPVPPG
ILNVGPGETNTLALSLWAQTPAGAQLSTVQLVDYGGRYETGFVQAFADDLQPQWTDRSEY
A

Enzyme Prediction     

EC	-	-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH35	37	352	2.6e-77	0.9218241042345277

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
396048	Glyco_hydro_35	1.22e-79	35	367	1	314	Glycosyl hydrolases family 35.
402180	BetaGal_dom2	1.09e-55	377	561	1	180	Beta-galactosidase, domain 2. This is the second domain of the five-domain beta-galactosidase enzyme that altogether catalyzes the hydrolysis of beta(1-3) and beta(1-4) galactosyl bonds in oligosaccharides as well as the inverse reaction of enzymatic condensation and trans-glycosylation. This domain is made up of 16 antiparallel beta-strands and an alpha-helix at its C-terminus. The fold of this domain appears to be unique. In addition, the last seven strands of the domain form a subdomain with an immunoglobulin-like (I-type Ig) fold in which the first strand is divided between the two beta-sheets. In penicillin spp this strand is interrupted by a 12-residue insertion which forms an additional edge-strand to the second beta-sheet of the sub-domain. The remainder of the second domain forms a series of beta-hairpins at its N-terminus, four strands of which are contiguous with part of the Ig-like sub-domain, forming in total a seven-stranded antiparallel beta-sheet. This domain is associated with family Glyco_hydro_35, pfam01301, which is N-terminal to it, but itself has no metazoan members.
198097	BetaGal_dom2	4.40e-39	376	562	1	182	Beta-galactosidase, domain 2. This is the second domain of the five-domain beta-galactosidase enzyme that altogether catalyses the hydrolysis of beta(1-3) and beta(1-4) galactosyl bonds in oligosaccharides as well as the inverse reaction of enzymatic condensation and trans-glycosylation. This domain is made up of 16 antiparallel beta-strands and an alpha-helix at its C terminus. The fold of this domain appears to be unique. In addition, the last seven strands of the domain form a subdomain with an immunoglobulin-like (I-type Ig) fold in which the first strand is divided between the two beta-sheets. In penicillin spp this strand is interrupted by a 12-residue insertion which forms an additional edge-strand to the second beta-sheet of the sub-domain. The remainder of the second domain forms a series of beta-hairpins at its N terminus, four strands of which are contiguous with part of the Ig-like sub-domain, forming in total a seven-stranded antiparallel beta-sheet. This domain is associated with family Glyco_hydro_35, which is N-terminal to it, but itself has no metazoan members.
404274	BetaGal_dom4_5	5.59e-30	858	980	1	109	Beta-galactosidase jelly roll domain. This domain is found in beta galactosidase enzymes. It has a jelly roll fold.
166698	PLN03059	1.18e-28	23	351	24	317	beta-galactosidase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UQC78589.1|GH35	0.0	24	1021	24	982
APA12177.1|GH35	0.0	21	1021	18	984
ATZ49456.1|GH35	0.0	21	1021	18	987
CCD45102.2|GH35	0.0	21	1021	18	987
QSZ31820.1|GH35	0.0	21	1021	18	983

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1TG7_A	5.91e-155	25	993	2	945	Native structure of beta-galactosidase from Penicillium sp. [Penicillium sp.],1XC6_A Native Structure Of Beta-Galactosidase from Penicillium sp. in complex with Galactose [Penicillium sp.]
5IFP_A	3.40e-154	25	991	42	979	Structure Of Beta-galactosidase From Aspergillus Niger [Aspergillus niger CBS 513.88]
5IFT_A	9.35e-154	25	991	42	979	STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 3-b-Galactopyranosyl glucose [Aspergillus niger CBS 513.88],5IHR_A Structure Of E298q-beta-galactosidase From Aspergillus Niger In Complex With Allolactose [Aspergillus niger CBS 513.88],5JUV_A STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 6-b-Galactopyranosyl galactose [Aspergillus niger CBS 513.88],5MGC_A STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 4-Galactosyl-lactose [Aspergillus niger CBS 513.88],5MGD_A STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 6-Galactosyl-lactose [Aspergillus niger CBS 513.88]
4IUG_A	2.40e-144	25	1017	42	1001	Crystal structure of beta-galactosidase from Aspergillus oryzae in complex with galactose [Aspergillus oryzae]
3OG2_A	9.56e-142	21	991	18	975	Chain A, Beta-galactosidase [Trichoderma reesei],3OGR_A Chain A, Beta-galactosidase [Trichoderma reesei],3OGS_A Chain A, Beta-galactosidase [Trichoderma reesei],3OGV_A Chain A, Beta-galactosidase [Trichoderma reesei]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|B6H5X9|BGALC_PENRW	0.0	26	1020	27	981	Probable beta-galactosidase C OS=Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) OX=500485 GN=lacC PE=3 SV=1
sp|B0Y752|BGALC_ASPFC	0.0	26	1021	29	983	Probable beta-galactosidase C OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=lacC PE=3 SV=1
sp|Q2UMD5|BGALC_ASPOR	0.0	16	1021	18	984	Probable beta-galactosidase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lacC PE=3 SV=1
sp|A1CE56|BGALC_ASPCL	0.0	26	1021	29	985	Probable beta-galactosidase C OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=lacC PE=3 SV=1
sp|A7EZS5|BGALC_SCLS1	0.0	21	1021	18	984	Probable beta-galactosidase C OS=Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1) OX=665079 GN=lacC PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.001060	0.998913	CS pos: 21-22. Pr: 0.9629

TMHMM  Annotations     

There is no transmembrane helices in RL4_JR_08590-RA-p1.