CAZyme Information: RL4_JR_06103-RA-p1

Basic Information

• Species: Raffaelea lauricola
• Lineage: Ascomycota; Sordariomycetes; ; Ophiostomataceae; Raffaelea; Raffaelea lauricola
• CAZyme ID: RL4_JR_06103-RA-p1
• CAZy Family: GH31
• CAZyme Description: unspecified product

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1078		120539.59	6.4888

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_RlauricolaRL4	10461	N/A	146	10315

• Gene Location: location=JACBXF010000155:25260-28544(-)

Full Sequence      

MVEHKNFAPVIAQPDWNNLAVIHRNTLTPRAAFFSYTSASDALGGGYANALAHCLSGTWK
FRLYQSPWHMPAEAAGALDPAAADLSDYGDISVPGMWQLQDAGGGVGPRYTNMALPFPVD
PPNVPLFENETGHYVRPFSVPSSFAGYQLRLRFEGVDAAFHVYVNGREIGYSQGSRNPSE
FDVTSAIRVDTSNILSVVVYKWCNGTYFEDQDQWWLSGIFRDVHLLAFPQTRIEDFKIET
HLSSTYTSAVLAIDVRLNTPATVSLELVDADGQKIAGAVKEGRDGSNVRGANQADSPGIH
AKFVVPVGEVHLWTAETPYLYRVLLSLQGVSQPQVICHAVGFRQVEIKDGVIVVNGRRIV
FRGTNRHEHHPQFGRAVPYEFMKNDLARMKQHNINAIRTSHYPNDFRLYDLADALGFWVM
DEADLETHGFSAVELAALTDEERNMSKKGVISLECGRATGWITNKLEYEASFIDRQRQIV
ARDKNHPSIIMWSLGNESYHGHNTIAAFNWVRAHDSTRLIHYEGDREAEVVDVYSLMYPE
IPEVLELLNTAPQKKPILLCEYLYGIGNGAGSISEYIDLFYRHPRLQGGFVWQWASQGLL
THTDEGSQAFYGYGGDFHDVINDGRENTHGYNFSDHTAKPALAELKQALQPVLVTVASIP
TATAVTITNRHDFSTLDYLRCAWRLVRDGTVSDYIELPLPAAIPPGASVELPVNIHQLPL
GDGYEQIETYLDIALSLKEASVWAPAGHTVAVAQFRLPQRPVAVILPLTPSSQQVPYVDR
QSSRISTIAGRESTWTFDLLQGKLISWTKSGDKEHLHQEQKRQNLLVSPPTLGFYRPLTD
HDAQGKGKEWTKKMLQHVQSHTRKVNAFTTDTAVEIEVIARAGPTGLEWGFHTKTKYSFT
ADAVRIAVHGKPFGVNAPKTLARIGLDLVLDPVLDRVAWFGRGPGESYRDRKQSQSVGNW
AMPIDDIFTLFEYPQESGNRTDVRHVTFSRSHDNDTGGCRLRAEFGSQDGCSFATSHYDV
QDIEKSKHPYELKRLRKTAPRVRLDWDHHGLGTAAIGPDTLPEYTLFNRTFNFDITLM

Enzyme Prediction     

EC	3.2.1.23:12

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	52	956	1.8e-198	0.9587765957446809

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
236548	lacZ	0.0	4	1077	5	1024	beta-galactosidase.
236673	ebgA	0.0	16	1078	4	998	cryptic beta-D-galactosidase subunit alpha; Reviewed
225789	LacZ	1.44e-129	55	955	15	804	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
397119	Glyco_hydro_2_C	1.17e-101	345	652	1	300	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
214988	Bgal_small_N	5.60e-66	790	1077	2	272	Beta galactosidase small chain. This domain comprises the small chain of dimeric beta-galactosidases EC:3.2.1.23. This domain is also found in single chain beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UPK96937.1|GH2	0.0	14	1077	12	1010
USW53106.1|CBM67|GH2	6.74e-316	13	1077	11	1033
QLI68281.1|GH2	2.06e-302	14	1077	12	1017
CAE7177124.1|GH2	8.02e-302	14	1077	11	1035
CBX97149.1|GH2	8.73e-300	14	1077	11	1030

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3OB8_A	7.22e-187	22	1077	22	1029	Structure of the beta-galactosidase from Kluyveromyces lactis in complex with galactose [Kluyveromyces lactis],3OB8_B Structure of the beta-galactosidase from Kluyveromyces lactis in complex with galactose [Kluyveromyces lactis],3OB8_C Structure of the beta-galactosidase from Kluyveromyces lactis in complex with galactose [Kluyveromyces lactis],3OB8_D Structure of the beta-galactosidase from Kluyveromyces lactis in complex with galactose [Kluyveromyces lactis],3OBA_A Structure of the beta-galactosidase from Kluyveromyces lactis [Kluyveromyces lactis],3OBA_B Structure of the beta-galactosidase from Kluyveromyces lactis [Kluyveromyces lactis],3OBA_C Structure of the beta-galactosidase from Kluyveromyces lactis [Kluyveromyces lactis],3OBA_D Structure of the beta-galactosidase from Kluyveromyces lactis [Kluyveromyces lactis]
6S6Z_A	8.98e-158	15	1077	3	979	Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_B Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_C Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_D Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_E Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_F Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_G Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_H Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8]
6SD0_A	9.20e-158	15	1077	4	980	Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_B Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_C Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_D Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8]
1F4A_A	3.15e-148	6	1075	4	1016	E. COLI (LACZ) BETA-GALACTOSIDASE (NCS CONSTRAINED MONOMER-ORTHORHOMBIC) [Escherichia coli],1F4A_B E. COLI (LACZ) BETA-GALACTOSIDASE (NCS CONSTRAINED MONOMER-ORTHORHOMBIC) [Escherichia coli],1F4A_C E. COLI (LACZ) BETA-GALACTOSIDASE (NCS CONSTRAINED MONOMER-ORTHORHOMBIC) [Escherichia coli],1F4A_D E. COLI (LACZ) BETA-GALACTOSIDASE (NCS CONSTRAINED MONOMER-ORTHORHOMBIC) [Escherichia coli],1F4H_A E. COLI (LACZ) BETA-GALACTOSIDASE (ORTHORHOMBIC) [Escherichia coli],1F4H_B E. COLI (LACZ) BETA-GALACTOSIDASE (ORTHORHOMBIC) [Escherichia coli],1F4H_C E. COLI (LACZ) BETA-GALACTOSIDASE (ORTHORHOMBIC) [Escherichia coli],1F4H_D E. COLI (LACZ) BETA-GALACTOSIDASE (ORTHORHOMBIC) [Escherichia coli]
5A1A_A	3.23e-148	6	1075	5	1017	2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor [Escherichia coli K-12],5A1A_B 2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor [Escherichia coli K-12],5A1A_C 2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor [Escherichia coli K-12],5A1A_D 2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P06864|BGA2_ECOLI	2.50e-186	16	1078	4	998	Evolved beta-galactosidase subunit alpha OS=Escherichia coli (strain K12) OX=83333 GN=ebgA PE=1 SV=4
sp|P00723|BGAL_KLULA	3.06e-186	22	1077	15	1022	Beta-galactosidase OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 GN=LAC4 PE=1 SV=2
sp|Q2XQU3|BGAL2_ENTCL	2.19e-164	10	1073	12	1021	Beta-galactosidase 2 OS=Enterobacter cloacae OX=550 GN=lacZ PE=3 SV=1
sp|A5F5U6|BGAL_VIBC3	1.32e-159	5	1077	2	1016	Beta-galactosidase OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395) OX=345073 GN=lacZ PE=3 SV=2
sp|Q56307|BGAL_THEMA	4.73e-157	15	1077	4	980	Beta-galactosidase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=lacZ PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.999961	0.000083

TMHMM  Annotations     

There is no transmembrane helices in RL4_JR_06103-RA-p1.