CAZyme Information: RAK76015.1

Basic Information

• Species: Aspergillus fijiensis
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus fijiensis
• CAZyme ID: RAK76015.1
• CAZy Family: GH135
• CAZyme Description: alpha,alpha-trehalose phosphate synthase subunit Tps3

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
926	KZ824652|CGC1	103944.23	6.1970

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AfijiensisCBS313.89	12336	1448319	318	12018

• Gene Location: location=KZ824652:231963-234966(+)

Full Sequence      

MTIYIASLFLPYTVDFHVNDPQNHEANTTTSPPPTNPVQSVAATPSAAVSLFERNNANVK
PGITPGPASEHEQFFAADIAKAELEHSGYPFPSTDREANLLTESEAHSPAWGSTLSLNQP
RPRAAFPASPSILKHQEPIAPGTEPAKEKIRTTPQPPTSSTTDHRRTQSRKSSFSDAEWT
IQTAEQGNGGLRNAVRSATDAGQLEDKVWVGTLGMPTDALPQSTKDSIARKLEEEYGSMT
VYVSDGDFDGHYTHFCKTILWPVFHYQIPDNPKSKAYEDHSWIYYVKTNQAFAEKIAQKW
KRGDSIWIQDYHLLLVPAMLRKLLPDAQIGFFLHIAFPSSEVFRCLAPRKELLEGILGAN
LIGFQTDEYCRHFLQTCSRILCVEATNEGLQLEDRFVNVGKFSIGIDPTSWDQRRRAADV
ERWIKTIAERYEGKRLIVSRDKIDQVRGIRQKLLSYELFLNTYPEWRDQVVLIQVATSTT
EQPELEATISDIAMRINSTHSTLAHQPLVFLKQDLAFPQYLALISLADALIITSLREGMN
LTSHEFVYCQDGKWGNNKKYGSLILSEFTGSASVFGNHALLVNPWDYRQCAEAIHTALSR
SETERKEVWTQMHQAVLQNSTANWVKSFSETLNRVWNEQSSREIMAVPRLQMNKLEEMYQ
KSNRRLIIVDYEGTLASWGSPKSIIVTTPQRAITTLAELTDDPRNVVYVMSARMPEEMER
LFRLVTGLGLIAENGCFVREPNSETWLKLTNKTQTDAWKTAVLQILDYYQERAEGSWIEQ
RHCSLVFHYGSAEDQVAAARLASECAGHINDACANQGVHAIPVEGALVVEPMGTNKASAT
ELVWRSCLKQSQQDQSVARPDFLLAIGDSRDDEPVFRWANKLESAQAINHVVTVTLGSRS
TEARATLTQGVTGVLSCLERLAEHKS

Enzyme Prediction     

EC	2.4.1.15:11

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT20	178	634	3e-159	0.9326315789473684

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
340820	GT20_TPS	0.0	180	628	19	458	trehalose-6-phosphate synthase. Trehalose-6-Phosphate Synthase (TPS, EC 2.4.1.15) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain.
395781	Glyco_transf_20	0.0	168	634	13	470	Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex,.
184712	PRK14501	1.98e-177	189	924	28	726	putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional
223457	OtsA	3.18e-163	209	640	64	486	Trehalose-6-phosphate synthase [Carbohydrate transport and metabolism].
215555	PLN03063	4.23e-128	179	867	32	707	alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCS07788.1|GT20	0.0	1	926	1	924
BCR95224.1|GT20	0.0	1	926	1	924
CAK46441.1|GT20	0.0	1	915	1	913
QMW41842.1|GT20	0.0	1	922	1	922
QRD86165.1|GT20	0.0	1	922	1	922

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6JBI_A	1.03e-99	214	635	47	465	Structure of Tps1 apo structure [Pyricularia oryzae 70-15],6JBI_B Structure of Tps1 apo structure [Pyricularia oryzae 70-15],6JBR_A Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_B Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_D Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_F Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_H Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_K Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_M Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_O Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBW_A Structure of Tps1/UDP complex [Pyricularia oryzae 70-15],6JBW_B Structure of Tps1/UDP complex [Pyricularia oryzae 70-15]
5DXF_A	1.62e-96	220	628	114	519	Structure of Candida albicans trehalose-6-phosphate phosphatase N-terminal domain [Candida albicans SC5314],5DXF_B Structure of Candida albicans trehalose-6-phosphate phosphatase N-terminal domain [Candida albicans SC5314]
5HVM_A	6.98e-96	180	635	24	477	Structure of Aspergillus fumigatus trehalose-6-phosphate synthase A in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293],5HVM_B Structure of Aspergillus fumigatus trehalose-6-phosphate synthase A in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293]
5HVO_A	2.69e-93	214	628	58	470	Structure of Aspergillus fumigatus trehalose-6-phosphate synthase B in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293],5HVO_B Structure of Aspergillus fumigatus trehalose-6-phosphate synthase B in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293],5HVO_C Structure of Aspergillus fumigatus trehalose-6-phosphate synthase B in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293],5HVO_D Structure of Aspergillus fumigatus trehalose-6-phosphate synthase B in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293]
5HUT_A	2.91e-88	188	643	31	478	Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP-glucose [Candida albicans SC5314],5HUT_B Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP-glucose [Candida albicans SC5314],5HUU_A Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and glucose-6-phosphate [Candida albicans SC5314],5HUU_B Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and glucose-6-phosphate [Candida albicans SC5314],5HVL_A Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and validoxylamine A [Candida albicans SC5314],5HVL_B Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and validoxylamine A [Candida albicans SC5314]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|O14081|TPSX_SCHPO	2.36e-200	167	921	183	941	Putative alpha,alpha-trehalose-phosphate synthase [UDP-forming] 106 kDa subunit OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC2E11.16c PE=1 SV=1
sp|Q9UUI7|TPSY_SCHPO	5.04e-194	4	921	5	889	Putative alpha,alpha-trehalose-phosphate synthase [UDP-forming] 100 kDa subunit OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC22F8.05 PE=1 SV=1
sp|P38426|TPS3_YEAST	9.16e-161	174	870	309	982	Trehalose synthase complex regulatory subunit TPS3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPS3 PE=1 SV=3
sp|P38427|TSL1_YEAST	1.43e-154	174	839	341	983	Trehalose synthase complex regulatory subunit TSL1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TSL1 PE=1 SV=1
sp|Q54K57|TPSA_DICDI	2.98e-122	175	869	33	691	Alpha,alpha-trehalose-phosphate synthase [UDP-forming] A OS=Dictyostelium discoideum OX=44689 GN=tpsA PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.910371	0.089654

TMHMM  Annotations     

There is no transmembrane helices in RAK76015.1.