CAZyme Information: QSL65536.1

Basic Information

• Species: Pneumocystis wakefieldiae
• Lineage: Ascomycota; Pneumocystidomycetes; ; Pneumocystidaceae; Pneumocystis; Pneumocystis wakefieldiae
• CAZyme ID: QSL65536.1
• CAZy Family: GT24
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1507	CP054538|CGC2	172311.99	7.7064

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Pwakefieldiae2A	3223	N/A	41	3182

• Gene Location: location=CP054538:28373-36352(-)

Full Sequence      

MLRHDVRIPVRFYGYVRKYIDEEGRTRYSWQDGEQVLAVASDVPIPGYGTNNTNNLRLWS
SRPMSEFDFSKFNAGDYESSVREQQRAETLSAVLYPNENVYQGKELRLKQQYFWVSASLH
DIIRRFKKSGKPWCKFPEQVFIQLNDTHPALAIVELQRILVDLELLEWDQAWSILYKPHR
SSTSEKWPIPMFQALLPRHMQIIYDINFFFLQSVEKKFPKDRDLLSRVSIVEESCPKYIR
MAFLAVIGSSKVNGVAKLHSSLIKTTIFKDFVKIFGPDKFVNITNGVTPRRWLHQANPSL
SNLISKKLGGYDFLTNLSKLKELEKYACDKEFQKEWRENHYLIFKRIHEYKRQTLNIFGV
IHRYLKLKAMSKEEILKQVPRVSIFGGQGFYVVNNDEDIGDIFKVHFIEDYNVSKAEIII
PASDISEHISTAGTEASGTSNMKFALNGGLIIGTVDGSNIEITREIGEENIFLFGNLTEN
VNNLRHKHVHENVPMDPELRKVCDAIESGTFGEPSLFAPLINALTYGHDYYLISDDFQSY
LNAHDLIDETYKNKDLWVYKTIVSVANMGFFSSDRTIQEYAEGIWNIEAYLGVLDETLKK
TNEITDKISTIFSTLNYRLEKVENTLKPIYKTTKSLIKTSENIQGTIVAIDRTRKYFNII
PEAEEIIKKGPNEDLTFFLNTLKKVKNSLVILRVSNFRSSEKSIVKLTQIWKGGCLQLYE
IFQKILLDHSVPIEPLYYTTKNLEFPVIPKDPLLNILLLSSFFYSTGFPESIKEIEKIYH
EIRAEYLSESLKLLSKGSLSTALKRGNNMYERNSNGIIIYTQALLGMFKSESLIIRDVFP
KNYQNVLLKSIHLAIVTYCDTVQRLNQHIHQNMSTDFALCYEIIEEISKIMEFINSYSNE
GPVIDELNKIMKIMENTGIYSLSDIIERIRKRVSSITSMPTDGDVSEITIESISRLLYIN
DYEETVIYLIMSMKNEEWNSFLFTDNKKAFDSNTDKKNVYRNFFSEVVDILYSQIDQKAR
ILLKKNSYLSLFLLNNLSYIEKNIKNTNLSNAISLNIFEKIAKTKNKYIDEYLETWKGCA
ENLLDVTYVRGGITNPVKLTLGSKERDAIKEKFKNFNNEFDELLKLNKMAVIHDTELKST
LVGEVKRIVVPLYTRFYDKLSLILRRQMFKTRSFLISALCSRRFSSITYNTSKVSILGAA
GGIGQPLSLLLKTNPLIGHLALYDIRGAPGVAADCNHINTPCKVVGYGEEDDGLIKALKD
TDVILIPAGVPRKPGMTRDDLFVTNASIVRNLAKVTADVAPDAHLLIISNPVNSTVPICA
EVYKKKGVYDPKRIFGVTTLDAVRASQFISDLKDIEPKDEQVHIVGGHSGVTIVPLLSQT
SHNFTDEEIKFLTNRIQYAGDEVVKAKNGSGSATLSMAFAASRFTNSLLRAMSGERNIIE
PTYVESPLFQDKGCHFFSTNVELGPEGVHKIHPLGNISLYERELLDLCFTELQKNIEKGI
KFVEETY

Enzyme Prediction     

EC	2.4.1.1:158	2.4.1.1:158

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT35	3	588	9.5e-178	0.8768545994065282

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
184948	PRK14986	0.0	3	588	183	811	glycogen phosphorylase; Provisional
133422	MDH_glyoxysomal_mitochondrial	0.0	1193	1503	2	310	Glyoxysomal and mitochondrial malate dehydrogenases. MDH is one of the key enzymes in the citric acid cycle, facilitating both the conversion of malate to oxaloacetate and replenishing levels of oxalacetate by reductive carboxylation of pyruvate. Members of this subfamily are localized to the glycosome and mitochondria. MDHs are part of the NAD(P)-binding Rossmann fold superfamily, which includes a wide variety of protein families including the NAD(P)-binding domains of alcohol dehydrogenases, tyrosine-dependent oxidoreductases, glyceraldehyde-3-phosphate dehydrogenases, formate/glycerate dehydrogenases, siroheme synthases, 6-phosphogluconate dehydrogenases, aminoacid dehydrogenases, repressor rex, and NAD-binding potassium channel domains, among others.
223136	GlgP	0.0	13	589	144	750	Glucan phosphorylase [Carbohydrate transport and metabolism].
395272	Phosphorylase	0.0	3	587	82	661	Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin.
273967	P_ylase	0.0	3	587	163	794	glycogen/starch/alpha-glucan phosphorylases. This family consists of phosphorylases. Members use phosphate to break alpha 1,4 linkages between pairs of glucose residues at the end of long glucose polymers, releasing alpha-D-glucose 1-phosphate. The nomenclature convention is to preface the name according to the natural substrate, as in glycogen phosphorylase, starch phosphorylase, maltodextrin phosphorylase, etc. Name differences among these substrates reflect differences in patterns of branching with alpha 1,6 linkages. Members include allosterically regulated and unregulated forms. A related family, TIGR02094, contains examples known to act well on particularly small alpha 1,4 glucans, as may be found after import from exogenous sources. [Energy metabolism, Biosynthesis and degradation of polysaccharides]

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QSL65536.1|GT35	0.0	1	1507	1	1507
EGX46445.1|GT35	3.63e-278	3	588	234	869
UDD59886.1|GT35	1.64e-272	3	588	102	737
AAU06197.1|GT35	2.44e-272	3	588	234	869
VDB83650.1|GT35	4.06e-272	3	588	244	879

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1YGP_A	1.40e-238	3	588	223	876	Phosphorylated Form Of Yeast Glycogen Phosphorylase With Phosphate Bound In The Active Site. [Saccharomyces cerevisiae],1YGP_B Phosphorylated Form Of Yeast Glycogen Phosphorylase With Phosphate Bound In The Active Site. [Saccharomyces cerevisiae]
3CEH_A	8.38e-158	3	588	171	806	Human liver glycogen phosphorylase (tense state) in complex with the allosteric inhibitor AVE5688 [Homo sapiens],3CEH_B Human liver glycogen phosphorylase (tense state) in complex with the allosteric inhibitor AVE5688 [Homo sapiens],3CEJ_A Human glycogen phosphorylase (tense state) in complex with the allosteric inhibitor AVE2865 [Homo sapiens],3CEJ_B Human glycogen phosphorylase (tense state) in complex with the allosteric inhibitor AVE2865 [Homo sapiens],3CEM_A Human glycogen phosphorylase (tense state) in complex with the allosteric inhibitor AVE9423 [Homo sapiens],3CEM_B Human glycogen phosphorylase (tense state) in complex with the allosteric inhibitor AVE9423 [Homo sapiens]
1FC0_A	2.44e-157	3	588	193	828	HUMAN LIVER GLYCOGEN PHOSPHORYLASE COMPLEXED WITH N-ACETYL-BETA-D-GLUCOPYRANOSYLAMINE [Homo sapiens],1FC0_B HUMAN LIVER GLYCOGEN PHOSPHORYLASE COMPLEXED WITH N-ACETYL-BETA-D-GLUCOPYRANOSYLAMINE [Homo sapiens],2ATI_A Glycogen Phosphorylase Inhibitors [Homo sapiens],2ATI_B Glycogen Phosphorylase Inhibitors [Homo sapiens]
1FA9_A	2.44e-157	3	588	193	828	HUMAN LIVER GLYCOGEN PHOSPHORYLASE A COMPLEXED WITH AMP [Homo sapiens]
2QLL_A	2.51e-157	3	588	194	829	Human liver glycogen phosphorylase- GL complex [Homo sapiens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P06738|PHSG_YEAST	2.30e-240	3	588	246	899	Glycogen phosphorylase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=GPH1 PE=1 SV=4
sp|Q00766|PHS1_DICDI	1.34e-179	3	588	210	841	Glycogen phosphorylase 1 OS=Dictyostelium discoideum OX=44689 GN=glpV PE=1 SV=3
sp|P34114|PHS2_DICDI	1.65e-175	3	590	280	910	Glycogen phosphorylase 2 OS=Dictyostelium discoideum OX=44689 GN=glpD PE=1 SV=2
sp|P0AC86|PHSG_ECOLI	1.36e-157	3	588	183	811	Glycogen phosphorylase OS=Escherichia coli (strain K12) OX=83333 GN=glgP PE=3 SV=1
sp|P0AC87|PHSG_SHIFL	1.36e-157	3	588	183	811	Glycogen phosphorylase OS=Shigella flexneri OX=623 GN=glgP PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000036	0.000000

TMHMM  Annotations     

There is no transmembrane helices in QSL65536.1.