CAZyme Information: QRD93652.1

Basic Information

• Species: Aspergillus flavus
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus flavus
• CAZyme ID: QRD93652.1
• CAZy Family: GT32
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2085		227095.71	4.9716

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AflavusNRRL3357_2020	13958	N/A	249	13709

• Gene Location: location=CP044623:696190-705179(+)

Full Sequence      

MTADEESPFLQSPPTPGSPVSDLERRRRSDRANKLVIYGSFVGVFLASADESLVISTWSS
IASQFNCLSQGSWLLVAYNFGYCVSLPVYGTLSDIYGRKNVLLWAYFLFALGCLACGASG
SLIQLILARVLAGISGGGMVALVSIIITDLMPSNEVALFRGYANVVNVAGRSLGAPVGGF
LIATLGWRWAFLGQLPLIIICILVAYYGLPSSLNQSKADSDQDDSRSRASDLDYGGIISL
ATAILILLFLIQSLSTTDDQPGLPYVLAPAFAIAIAVFVLTEAYWARKPLIPLSLLKCSL
GGYCVGQLLLITGRSALSSNMVPYFVRIEKVTDFLASFTYVVTAVGVSVGGLISGAIIKR
TKQYKTMTVIAVVSTVLLSILIFIRYRDGCYTWELIYLFPSGLSNGILFSTQFIGMSLAA
PKERLATSIGIYYLSQQLGFIVGPAASVAVVQRLFAHRLSESLEGLEEKQCIGKVINDLR
YSESLPERLQSIVRSSYLYGFQFVPFALGNWEAAYEKASAFVSGLTTDQKLALITGSNVE
STNGNFTPLYFLDGDMGLQDFYYVSAFSLSSALAMTWDRDAIYEQAKAVGSEFYNKGVQV
VAGPTSQPLGRTPWGGRGVEGFGPDPYLNGLATGLTTKGYVDAGVIPGGKHFLLYEQETN
RTSSFGSSGEGSPYSSNADDKTIHETYLWPFYDAVKNGAGAVMCAMTKVNGTMACENSDL
LMKMLKTELGFPGMVWPDMNGQNSAKGSALGGEDYGSSSIWSTSTMESFLSNGTLSEARL
NDMAIRNLIGYYYVNLDNGRQPTRQTTDVYVDVRANHSKLIRENGAKSMALLKNEGVLPL
SKPRVMSIFGAHAGPIMGGPNSNVDVMGSGPTYQGHLATGSGSGMASMPYLITPYGALTN
KAAQDGTVLRWVLNDTYSSGGGSSLVPSSTSSTAVEPSFENFATGSDICLVFINALAGEG
ADRTELYNADQDAMVNTVADNCNNTVAVVNTVGPRLLDQWIEHDNVTAVLYGSLLGQESG
NSIVDLLYGDVNPSGRLVHTIAKNESDYNVGLCYTAQCNFTEGVYLDYRYFDAHNITPRY
PFGHGLSYTTFHYSSLAIKAPSSITKAPKGNLTVGGPSDLWDVVGTVSARIANNGTLSGA
EVPQLYLGFPDSADQPVPVWVAQNEPVGVLRAAHDLANDFGRVLGVNGTVNVFDTDISES
PGKAVIITGTVGQSTLIDQLVSDGKLDVSLIEGKWESYVSQVVENPFPNVTWSLVVAGSD
RRGTIYGLYDISEQMGVSPWYWWADVPVKTKTGIWVAPEGTFQKSPSVKYRGFFINDESP
ALSGWVAENFGTKFNSAFYRHIFELCLRLKGNYLWPAMWGKMFYVDDVENGQLAHEYGII
MGTSHHEPMARSEQEQKTYLDGEWNWNENQANITTFFQQGIDRAKHWDTIWTMGMRGEGD
VASPTLTAADLEELIHAQQKLLVDSFNASDPASIPQTWVLYKEVSDYYAAGMEVPESVTL
LWTDDNSGNLIRVPIANETSRVAGAGVYYHFDYVGSPRSYKWINTIQLVKTWEQMHLAYQ
KSARQIWIANVGDIKGLEVPLTHFMDMAYDMDRFTSTDSTTKWLKRWAAREFNERIADRT
ADILNHYGTLVARRKYELLSQLPFAFSTIYYDEAEANLAQWESLLAQTQSVYSSLDQATR
TPFFEMVLHPVLAGKTVVDLYTKTAFNALYHQQGRISTNRLAQKVHDLFTEDSAITERYH
GVNGGKWRPVMDQVHIGYSSWDDPVDNTNVMPALSYIAAPQGAGGIGIAVQGSAASYPEQ
KTLRLLSMDPYMPPSESRYIDIFARKNTTVTYSISSNTSYVTVSNASGSVSASSNISDVR
STITVDWASAEPGLSHTELQVRAGDGFTAALVLSVNNTFVPTNFTGFVESNGVISIEASH
YTSAETKSNVSYVEIPHYGRTLSAVMPWPVTMGTQHPNTGPALRYSLYTTTASPSARLII
SLGASHNHDPTRLIKFAYSLDGSVPVTVRPVSTVPPYKEGQAWQQAVIENGWTSVIELPG
EVGTGEHELSLWLLEPGVVLQKIVLDMGGYQDSALGPPESMRVGV

Enzyme Prediction     

EC	-	3.2.1.131:6

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH115	1153	1789	6.4e-242	0.8436154949784792
GH3	544	745	3.9e-39	0.8796296296296297

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
406396	Glyco_hydro_115	0.0	1304	1638	1	334	Glycosyl hydrolase family 115. Glyco_hydro_115 is a family of glycoside hydrolases likely to have the activity of xylan a-1,2-glucuronidase, EC:3.2.1.131, or a-(4-O-methyl)-glucuronidase EC:3.2.1.-.
407695	GH115_C	8.92e-65	1906	2079	1	172	Gylcosyl hydrolase family 115 C-terminal domain. This domain is found at the C-terminus of glycosyl hydrolase family 115 proteins. This domain has a beta-sandwich fold.
341045	MFS_Azr1_MDR_like	1.41e-52	41	443	2	312	Saccharomyces cerevisiae Azole resistance protein 1 (Azr1p), and similar multidrug resistance (MDR) transporters of the Major Facilitator Superfamily. This subfamily is composed of multidrug resistance (MDR) transporters including various Saccharomyces cerevisiae proteins such as azole resistance protein 1 (Azr1p), vacuolar basic amino acid transporter 1 (Vba1p), vacuolar basic amino acid transporter 5 (Vba5p), and Sge1p (also known as Nor1p, 10-N-nonyl acridine orange resistance protein, and crystal violet resistance protein). MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. This subfamily belongs to the Methylenomycin A resistance protein (also called MMR peptide) and similar multidrug resistance (MDR) transporters (MMR-like MDR transporter) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.
396478	Glyco_hydro_3_C	2.85e-47	829	1068	1	216	Glycosyl hydrolase family 3 C-terminal domain. This domain is involved in catalysis and may be involved in binding beta-glucan. This domain is found associated with pfam00933.
369468	MFS_1	7.92e-40	41	444	2	346	Major Facilitator Superfamily.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRD93652.1|GH115|GH3	0.0	1	2085	1	2085
QMW34550.1|GH115	0.0	1159	2085	41	967
UDD63493.1|GH115	0.0	1159	2085	41	967
QMW46604.1|GH115	0.0	1159	2085	41	967
BAE56806.1|GH115	0.0	1159	2079	41	961

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4ZMH_A	5.46e-194	1161	2084	27	937	Crystal structure of a five-domain GH115 alpha-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T [Saccharophagus degradans 2-40],4ZMH_B Crystal structure of a five-domain GH115 alpha-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T [Saccharophagus degradans 2-40]
4C90_A	9.73e-145	1156	2080	52	849	Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C90_B Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C91_A Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C91_B Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus]
7PUG_A	4.96e-139	1156	2080	25	834	Chain A, xylan alpha-1,2-glucuronidase [uncultured bacterium]
7PXQ_A	3.16e-138	1156	2080	24	833	Chain A, xylan alpha-1,2-glucuronidase [uncultured bacterium]
6NPS_A	6.21e-132	1168	2084	31	967	Crystal structure of GH115 enzyme AxyAgu115A from Amphibacillus xylanus [Amphibacillus xylanus NBRC 15112],6NPS_B Crystal structure of GH115 enzyme AxyAgu115A from Amphibacillus xylanus [Amphibacillus xylanus NBRC 15112]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q2UNR0|BGLD_ASPOR	0.0	507	1157	34	684	Probable beta-glucosidase D OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=bglD PE=3 SV=1
sp|B8NJF4|BGLD_ASPFN	0.0	507	1157	34	684	Probable beta-glucosidase D OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=bglD PE=3 SV=2
sp|A2QPK4|BGLD_ASPNC	0.0	508	1157	34	686	Probable beta-glucosidase D OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=bglD PE=3 SV=2
sp|Q5AUW5|BGLD_EMENI	1.96e-300	507	1157	34	744	Probable beta-glucosidase D OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=bglD PE=3 SV=2
sp|A5ABF5|BGLM_ASPNC	9.54e-130	475	1151	10	698	Probable beta-glucosidase M OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=bglM PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.999466	0.000557

TMHMM  Annotations     

Start	End
35	56
71	93
100	122
126	148
161	183
187	209
232	254
264	286
293	315
335	357
369	386
396	418
430	452