CAZyme Information: QRD06231.1

Basic Information

• Species: Parastagonospora nodorum
• Lineage: Ascomycota; Dothideomycetes; ; Phaeosphaeriaceae; Parastagonospora; Parastagonospora nodorum
• CAZyme ID: QRD06231.1
• CAZy Family: GT2
• CAZyme Description: Cytochrome b561 domain-containing protein [Source:UniProtKB/TrEMBL;Acc:A0A7U2IAF8]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
425		43774.84	10.0089

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PnodorumSN15	17580	321614	132	17448

• Gene Location: location=CP069042:346671-348066(+)

Full Sequence      

MKLNGATTSALLAFTSQVSAQVASVCPSKNVCFKLNIPDSTASSGSGDIFFQISAPSSHE
WVALGQGTSMAGSNMFLVYTSANGNNVTLSPRTASGYVPPDLNSEAQVTLLEGSGVANGI
MTANVKCSNCNSWSGGTMDFKSSKGNWVYAFNPSNGPKNSDSQSASIRQHTGHDTFSWDF
ATAKGGNSVNPLLTAAPVAPGAGSGAGPAGPGNASGGPGGITSTGGGSSNRRAMLIAHGV
LASLAFVILFPAGAIAIRLASVPGIAWIHGGFQIFAYMVYIAAAGLGIHLACGLGLLKSY
HPVIGLVVLAVLFFQPILGAVHHRLFKVHNGRTLWSYGHIWLGRAAITLGIINGGLGLRL
ANNTNSGKIAYGVIAGFMWLAWVAAMVIGEKRRKTAVHHKERTGSDGSEGAVVPPTNGHY
EPKQT

Enzyme Prediction     

No EC number prediction in QRD06231.1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
AA8	27	184	4.4e-23	0.8791208791208791

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
187688	CDH_like_cytochrome	2.61e-56	25	180	15	168	Heme-binding cytochrome domain of fungal cellobiose dehydrogenases. Cellobiose dehydrogenase (CellobioseDH or CDH) is an extracellular fungal oxidoreductase that degrades both lignin and cellulose. Specifically, CDHs oxidize cellobiose, cellodextrins, and lactose to corresponding lactones, utilizing a variety of electron acceptors. Class-II CDHs are monomeric hemoflavoenzymes that are comprised of a b-type cytochrome domain linked to a large flavodehydrogenase domain. The cytochrome domain of CDH and related enzymes, which this model describes, folds as a beta sandwich and complexes a heme molecule. It is found at the N-terminus of this family of enzymes, and belongs to the DOMON domain superfamily, a ligand-interacting motif found in all three kingdoms of life.
176490	Cyt_b561_FRRS1_like	5.98e-39	214	384	15	188	Eukaryotic cytochrome b(561), including the FRRS1 gene product. Cytochrome b(561), as found in eukaryotes, similar to and including the human FRRS1 gene product (ferric-chelate reductase 1), also called SDR-2 (stromal cell-derived receptor 2). This family comprises a variety of domain architectures, many of which contain dopamine beta-monooxygenase (DOMON) domains. The protein might act as a ferric-chelate reductase, catalyzing the reduction of Fe(3+) to Fe(2+), such as associated with the transport of iron from the endosome to the cytoplasm. It is assumed that this protein uses ascorbate as the electron donor. Belongs to the cytochrome b(561) family, which are secretory vesicle-specific electron transport proteins. Cytochromes b(561) are integral membrane proteins that bind two heme groups non-covalently, and may have six alpha-helical trans-membrane segments.
406418	CDH-cyt	5.85e-32	30	184	19	174	Cytochrome domain of cellobiose dehydrogenase. CDH-cyt is the cytochrome domain, at the N-terminus, of cellobiose dehydrogenase. CDH-cyt folds as a beta sandwich with the topology of the antibody Fab V(H) domain and binds iron. The haem iron is ligated by Met83 and His181 in UniProtKB:Q01738.
214769	B561	8.87e-13	238	358	2	129	Cytochrome b-561 / ferric reductase transmembrane domain. Cytochrome b-561 recycles ascorbate for the generation of norepinephrine by dopamine-beta-hydroxylase in the chromaffin vesicles of the adrenal gland. It is a transmembrane heme protein with the two heme groups being bound to conserved histidine residues. A cytochrome b-561 homologue, termed Dcytb, is an iron-regulated ferric reductase in the duodenal mucosa. Other homologues of these are also likely to be ferric reductases. SDR2 is proposed to be important in regulating the metabolism of iron in the onset of neurodegenerative disorders.
176491	Cyt_b561_CYB561D2_like	1.26e-05	238	352	24	149	Eukaryotic cytochrome b(561), including the CYB561D2 gene product. Cytochrome b(561), as found in eukaryotes, similar to and including the human CYB561D2 gene product. CYB561D2 is a candidate tumor suppressor. The protein might act as a ferric-chelate reductase, catalyzing the reduction of Fe(3+) to Fe(2+), such as associated with the transport of iron from the endosome to the cytoplasm. It is assumed that this protein uses ascorbate as the electron donor. Belongs to the cytochrome b(561) family, which are secretory vesicle-specific electron transport proteins. Cytochromes b(561) are integral membrane proteins that bind two heme groups non-covalently, and may have six alpha-helical trans-membrane segments.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CAE7013938.1|AA8	1.78e-165	11	394	11	385
SMR42136.1|AA0	1.72e-109	8	408	7	401
SMR44317.1|AA0	1.72e-109	8	408	7	401
SMR55355.1|AA0	2.81e-102	31	395	25	367
SMR57731.1|AA0	2.81e-102	31	395	25	367

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1D7B_A	5.70e-08	38	192	33	185	Chain A, CELLOBIOSE DEHYDROGENASE [Phanerodontia chrysosporium],1D7B_B Chain B, CELLOBIOSE DEHYDROGENASE [Phanerodontia chrysosporium],1D7D_A Chain A, CELLOBIOSE DEHYDROGENASE [Phanerodontia chrysosporium],1D7D_B Chain B, CELLOBIOSE DEHYDROGENASE [Phanerodontia chrysosporium]
1D7C_A	6.05e-08	38	192	33	185	Chain A, CELLOBIOSE DEHYDROGENASE [Phanerodontia chrysosporium],1D7C_B Chain B, CELLOBIOSE DEHYDROGENASE [Phanerodontia chrysosporium]
1PL3_A	1.91e-07	38	192	33	185	Chain A, Cellobiose dehydrogenase [Phanerodontia chrysosporium],1PL3_B Chain B, Cellobiose dehydrogenase [Phanerodontia chrysosporium]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q9M363|B561I_ARATH	1.05e-09	238	383	220	366	Cytochrome b561 and DOMON domain-containing protein At3g61750 OS=Arabidopsis thaliana OX=3702 GN=At3g61750 PE=3 SV=1
sp|Q9SV71|B561O_ARATH	7.47e-08	223	393	205	384	Cytochrome b561 and DOMON domain-containing protein At4g12980 OS=Arabidopsis thaliana OX=3702 GN=At4g12980 PE=2 SV=1
sp|Q9LSE7|B561C_ARATH	9.61e-07	238	393	220	383	Cytochrome b561 and DOMON domain-containing protein At3g25290 OS=Arabidopsis thaliana OX=3702 GN=At3g25290 PE=2 SV=1
sp|Q01738|CDH_PHACH	1.89e-06	38	192	51	203	Cellobiose dehydrogenase OS=Phanerodontia chrysosporium OX=2822231 GN=CDH-1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.039427	0.960544	CS pos: 20-21. Pr: 0.8722

TMHMM  Annotations     

Start	End
237	259
274	296
303	325
340	362
369	388