CAZyme Information: QRD02160.1

Basic Information

• Species: Parastagonospora nodorum
• Lineage: Ascomycota; Dothideomycetes; ; Phaeosphaeriaceae; Parastagonospora; Parastagonospora nodorum
• CAZyme ID: QRD02160.1
• CAZy Family: GH31
• CAZyme Description: Mannosyl-oligosaccharide glucosidase [Source:UniProtKB/TrEMBL;Acc:A0A7U2FBB7]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
836		94301.10	6.5874

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PnodorumSN15	17580	321614	132	17448

• Gene Location: location=CP069035:794351-797087(+)

Full Sequence      

MLLPPPLLALLCAILPPASAAIDSASSNNASLLWGPYRPNLYMGIRPRVPDSLIAGLMWG
KEEEIESKLRHNVDISAGMARYGWTSYDTREGGTQTIEDVQNKIDITTEFIKKSEGSSAG
NWALRIRGTPRKDAPADLKTTVVFYVGMESFDTCTKCKLESSEQVGAEEDTSIHAVNLWM
EHPQLGTASMHIPASVGENGRHEAMIVKSMNVSEDKLWRANTTFLDALKDQMKDKKEDAA
MDPMLHNEIGNGNMHFVQMNCHGKFEFDVLYSNHAAARAMTAAELTRKLESTVKSFGKTF
VHVFNPKTPFRDEQHLEFAEYVLSNLLGGLGYFAGTNQVDTSKKAIYAETTGKFWEKSAE
AKSHATPEIRGPYELLTHTPSRAMFPRGFLWDEGYHLLPVLEWDADLAIEVVRNWLALMD
DDGWIAREQILGVEAEFATPADFITQFPHIANPPTMFLVISKLIDMLGSKTKYYGHESWY
LTKADAGKTFIVDVYPLLKRHYEWYRKSQSGDVEAHSVPSANLNEGYRWRGRTPETNFAS
GLDDYPRAEPPDITELHVDALCWVGAMARVLEKIADYLNQTDSLPYQTHLRGVQTNLDAL
HWDDKQSIYCDAVVRDSAHSLVCHKGYISLFPFMTGFVGASHPHLPATLDLLRDSAHLWT
DHGIRSLSPESPKYGVGDNYWRSPIWVNMNFMILEQLLVLARTAGPEQQRCRDIYVELRA
NIVHTVYSAWLQTGYIWEQYDPVGGHGQRTQHFTGWSALVVQIMAMPDLAGAEGYKERVK
GYYEEAKKTAVQNRKSSTGLVVGVGMLVVFVYVTRRRFMGTWRGLKRKHRRVSSNE

Enzyme Prediction     

EC	3.2.1.106:25	3.2.1.-:4

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH63	554	764	2.7e-27	0.43333333333333335

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397353	Glyco_hydro_63	5.96e-178	284	766	5	494	Glycosyl hydrolase family 63 C-terminal domain. This is a family of eukaryotic enzymes belonging to glycosyl hydrolase family 63. They catalyze the specific cleavage of the non-reducing terminal glucose residue from Glc(3)Man(9)GlcNAc(2). Mannosyl oligosaccharide glucosidase EC:3.2.1.106 is the first enzyme in the N-linked oligosaccharide processing pathway. This family represents the C-terminal catalytic domain.
407154	Glyco_hydro_63N	1.67e-64	32	234	1	209	Glycosyl hydrolase family 63 N-terminal domain. This is a family of eukaryotic enzymes belonging to glycosyl hydrolase family 63. They catalyze the specific cleavage of the non-reducing terminal glucose residue from Glc(3)Man(9)GlcNAc(2). Mannosyl oligosaccharide glucosidase EC:3.2.1.106 is the first enzyme in the N-linked oligosaccharide processing pathway. This family represents the N-terminal beta sandwich domain.
225942	GDB1	9.60e-08	546	761	411	599	Glycogen debranching enzyme (alpha-1,6-glucosidase) [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRD02160.1|GH63	0.0	1	836	1	836
KAF2833894.1|GH63	0.0	26	830	26	833
KAF1849764.1|GH63	0.0	23	829	29	836
KAF1921735.1|GH63	0.0	79	827	1	750
KAF1937561.1|GH63	0.0	23	828	23	829

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4J5T_A	1.50e-144	29	764	12	800	Crystal structure of Processing alpha-Glucosidase I [Saccharomyces cerevisiae S288C]
5MHF_A	9.48e-107	23	765	27	776	Murine endoplasmic reticulum alpha-glucosidase I with N-9'-methoxynonyl-1-deoxynojirimycin [Mus musculus],5MHF_B Murine endoplasmic reticulum alpha-glucosidase I with N-9'-methoxynonyl-1-deoxynojirimycin [Mus musculus],5MHF_C Murine endoplasmic reticulum alpha-glucosidase I with N-9'-methoxynonyl-1-deoxynojirimycin [Mus musculus],5MHF_D Murine endoplasmic reticulum alpha-glucosidase I with N-9'-methoxynonyl-1-deoxynojirimycin [Mus musculus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|O14255|GCS1_SCHPO	1.24e-180	23	765	33	805	Probable mannosyl-oligosaccharide glucosidase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC6G10.09 PE=3 SV=1
sp|P53008|CWH41_YEAST	1.40e-143	29	764	42	830	Mannosyl-oligosaccharide glucosidase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=CWH41 PE=1 SV=1
sp|Q84M89|GCS2_ARATH	3.78e-109	31	765	45	783	Alpha-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=GCS2 PE=2 SV=1
sp|F4HTM3|GCS1_ARATH	1.17e-108	31	765	108	846	Mannosyl-oligosaccharide glucosidase GCS1 OS=Arabidopsis thaliana OX=3702 GN=GCS1 PE=1 SV=1
sp|Q80UM7|MOGS_MOUSE	5.75e-108	23	765	82	831	Mannosyl-oligosaccharide glucosidase OS=Mus musculus OX=10090 GN=Mogs PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000212	0.999732	CS pos: 20-21. Pr: 0.9791

TMHMM  Annotations     

Start	End
796	813