CAZyme Information: QRD01347.1

Basic Information

• Species: Parastagonospora nodorum
• Lineage: Ascomycota; Dothideomycetes; ; Phaeosphaeriaceae; Parastagonospora; Parastagonospora nodorum
• CAZyme ID: QRD01347.1
• CAZy Family: GH27
• CAZyme Description: 1,3-beta-glucanosyltransferase [Source:UniProtKB/TrEMBL;Acc:A0A7U2FA35]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
537		57011.29	5.3777

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PnodorumSN15	17580	321614	132	17448

• Gene Location: location=CP069034:523830-525592(+)

Full Sequence      

MRGLFAAAALLVGAQAAIDPIVIKGQKFFYKTNGTQFFMKGVAYQQEINSNTTQSAESTI
QITDPLADATACKRDIEYLKKIQTNTIRVYAIDPSKDHKACMNALADAGIYVVADLSEPK
NSIIRDDPGWTTTLYERYTQVVDELMKYDNTLGFFAGNEVSNQPNNTIASAFVKAAVRDT
KAYIKSKNYRPVGVGYATNDDADIRENLSAYFDCGNKEDAIDFWGYNIYSWCGDSSFQES
GFDVRTKEFEKYNVPVFFAEYGCNTPRPRLFTEVGALYGKQMTGVWSGGIVYMYFEEENK
FGLASINNGKVSTNKDFENLSKEIAKATPVGVKMSEYNPTNTAAATCPKVTQGKWEAKSD
PLPPSANAPLCSCMVETLSCVVADNVQEKDFGDMFGFICGEKKGQYCQGINKNATSGPYG
AYGMCSAKDQLSFAVNAYAKAVSNGCGFKGKATTKAAVATPSASGCATLLQAAGAQGTGV
VSGGAQAGASGAAASGSAGAAAGAAAGLSVPSFSIGSFGMGIYVLGAMASGMAMILL

Enzyme Prediction     

EC	2.4.1.-:3	2.4.1.-:33

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH72	15	326	1.5e-134	0.9903846153846154

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397351	Glyco_hydro_72	9.01e-159	14	327	5	314	Glucanosyltransferase. This is a family of glycosylphosphatidylinositol-anchored beta(1-3)glucanosyltransferases. The active site residues in the Aspergillus fumigatus example are the two glutamate residues at 160 and 261.
400371	X8	3.85e-21	378	453	1	76	X8 domain. The X8 domain domain contains at least 6 conserved cysteine residues that presumably form three disulphide bridges. The domain is found in an Olive pollen allergen as well as at the C-terminus of several families of glycosyl hydrolases. This domain may be involved in carbohydrate binding. This domain is characteristic of GPI-anchored domains.
197867	X8	2.40e-11	378	466	1	83	Possibly involved in carbohydrate binding. The X8 domain, which may be involved in carbohydrate binding, is found in an Olive pollen antigen as well as at the C terminus of family 17 glycosyl hydrolases. It contains 6 conserved cysteine residues which presumably form three disulfide bridges.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRD01347.1|CBM43|GH72	0.0	1	537	1	537
CAE7209865.1|CBM43|GH72	7.15e-284	1	537	1	537
CBX98350.1|CBM43|GH72	2.39e-278	23	482	336	795
AQA29359.1|CBM43|GH72	3.28e-203	6	481	12	489
UJO23972.1|CBM43|GH72	3.28e-203	6	481	12	489

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2W62_A	4.55e-129	21	432	36	449	Saccharomyces cerevisiae Gas2p in complex with laminaripentaose [Saccharomyces cerevisiae],2W63_A Saccharomyces Cerevisiae Gas2p In Complex With Laminaritriose And Laminaritetraose [Saccharomyces cerevisiae],5O9O_A Crystal structure of ScGas2 in complex with compound 7. [Saccharomyces cerevisiae S288C],5O9P_A Crystal structure of Gas2 in complex with compound 10 [Saccharomyces cerevisiae S288C],5O9Q_A Crystal structure of ScGas2 in complex with compound 6 [Saccharomyces cerevisiae S288C],5O9R_A Crystal structure of ScGas2 in complex with compound 9 [Saccharomyces cerevisiae S288C],5O9Y_A Crystal structure of ScGas2 in complex with compound 11 [Saccharomyces cerevisiae S288C],5OA2_A Crystal structure of ScGas2 in complex with compound 8 [Saccharomyces cerevisiae S288C],5OA2_B Crystal structure of ScGas2 in complex with compound 8 [Saccharomyces cerevisiae S288C],5OA2_C Crystal structure of ScGas2 in complex with compound 8 [Saccharomyces cerevisiae S288C],5OA6_A Crystal structure of ScGas2 in complex with compound 12 [Saccharomyces cerevisiae S288C]
2W61_A	1.28e-128	21	432	36	449	Saccharomyces cerevisiae Gas2p apostructure (E176Q mutant) [Saccharomyces cerevisiae]
5FIH_A	1.28e-128	21	432	36	449	SACCHAROMYCES CEREVISIAE GAS2P (E176Q MUTANT) IN COMPLEX WITH LAMINARITETRAOSE AND LAMINARIPENTAOSE [Saccharomyces cerevisiae]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|D4ATC3|GAS1_ARTBC	5.46e-194	4	482	6	498	1,3-beta-glucanosyltransferase ARB_07487 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_07487 PE=1 SV=1
sp|B0XVI5|GEL4_ASPFC	4.38e-176	1	474	1	487	1,3-beta-glucanosyltransferase gel4 OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=gel4 PE=1 SV=1
sp|P0C956|GEL4_ASPFU	4.38e-176	1	474	1	487	1,3-beta-glucanosyltransferase gel4 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=gel4 PE=3 SV=1
sp|P0C955|GEL3_ASPFU	4.14e-165	10	482	5	477	1,3-beta-glucanosyltransferase gel3 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=gel3 PE=3 SV=1
sp|B0XT09|GEL3_ASPFC	4.57e-165	10	482	5	477	1,3-beta-glucanosyltransferase gel3 OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=gel3 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000297	0.999680	CS pos: 16-17. Pr: 0.9769

TMHMM  Annotations     

Start	End
514	536