CAZyme Information: QRC96987.1

Basic Information

• Species: Parastagonospora nodorum
• Lineage: Ascomycota; Dothideomycetes; ; Phaeosphaeriaceae; Parastagonospora; Parastagonospora nodorum
• CAZyme ID: QRC96987.1
• CAZy Family: CE5
• CAZyme Description: Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase [Source:UniProtKB/TrEMBL;Acc:A0A7U2I2I2]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
418		47980.60	9.6034

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PnodorumSN15	17580	321614	132	17448

• Gene Location: location=CP069029:50682-52123(+)

Full Sequence      

MSLINRVRDLATNDEHTRWMIPLLLVVDAALCGVVIEKIPYTEIDWTTYMQHIALIIKGE
RDYTKITGSTGPLVYPGAHVWIYKQLFKITDEGRDIQRAQYIFALVYLGTLALVFQCYRK
ARVPPYVFPLLILSKRLHSIFLLRCFNDCFAVLGLFAALFCYQRDQWHVGSFLFATGLNV
KMSLLLPLPAMGVLMIMKLGSREAMTHAMIIFQTTVLFGYPFRKAAFSYFGRAFELSRQF
TYKWTVNWRFVSEETFLSKPFALGLLSVHVTLLITFFLTRWIKPSKRTPKQFLKIIMPQA
EPRDQDTMALRITPNFTMTVLLTAMTIGMLCARSLHYQFFAYIAWATPFLLWKAGFHPVL
QYALWAAQEWAWNVYPSTSVSSGVVVAVLAVTVGGVWWGTRDEYVGELKGVVGHDHED

Enzyme Prediction     

EC	2.4.1.258:10

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT58	22	382	1.6e-139	0.9945054945054945

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
398745	ALG3	0.0	22	376	1	352	ALG3 protein. The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl pyrophosphate. Whereas early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions from Man5GlcNAc2-PP-Dol to Man9GlcNAc2-PP-Dol on the lumenal side use Dol-P-Man. ALG3 gene encodes the Dol-P-Man:Man5GlcNAc2-PP-Dol mannosyltransferase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRC96987.1|GT58	2.47e-309	1	418	1	418
CBX89957.1|GT58	5.89e-233	1	417	89	505
CAE7199648.1|GT58	2.88e-227	2	416	7	421
QDS73095.1|GT58	7.42e-160	1	401	13	415
QBZ56825.1|GT58	5.84e-157	13	404	21	418

PDB Hits     

QRC96987.1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q0TVF9|ALG3_PHANO	1.20e-223	1	315	292	606	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) OX=321614 GN=ALG3 PE=3 SV=2
sp|Q9C1K8|ALG3_NEUCR	1.80e-148	13	409	27	431	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=alg-3 PE=3 SV=1
sp|Q2U6A4|ALG3_ASPOR	2.90e-140	1	402	1	397	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alg3 PE=3 SV=1
sp|Q4WVG2|ALG3_ASPFU	1.23e-135	1	396	9	399	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=alg3 PE=3 SV=1
sp|A1CBE6|ALG3_ASPCL	1.62e-133	1	407	63	464	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=alg3 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.985517	0.014491

TMHMM  Annotations     

Start	End
101	118
140	162
169	191
204	222
260	282
310	332
339	361
376	398