CAZyme Information: QRC92248.1

Basic Information

• Species: Parastagonospora nodorum
• Lineage: Ascomycota; Dothideomycetes; ; Phaeosphaeriaceae; Parastagonospora; Parastagonospora nodorum
• CAZyme ID: QRC92248.1
• CAZy Family: AA7
• CAZyme Description: Beta-mannosidase [Source:UniProtKB/TrEMBL;Acc:A0A7U2HVI9]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
852		98068.14	6.2198

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PnodorumSN15	17580	321614	132	17448

• Gene Location: location=CP069024:1254872-1257823(-)

Full Sequence      

MRYSFTRAKVNRMTNAQSLSSVTLTKGWQFKQTNHAGEDAWMTVERVPTNVHLDLIEHGK
IPDPFLGFNELQAEWVADESWTYKVELPKVQETKDGVVHVLAFDGLDTFAKVKLNGKMIL
ESDNMFIPHRIDITKKLEVGATNVLEIDFASARLEAIKIKDQHPEHKWVGFNGDMARLAV
RKAQYHWGWDWGPILNTCGPWREVKLETYQSRVEDLRIDYKLDNSLKTVQGTISARVEGS
AAQKVTFAVYDGDRMTFKESANINDGIAKVEFHVNSPKLWYPHGYGEQPLYKITASVSIG
EIEVHQVSRRTGFRKSELIQEPDAIGKSFYFRVNGIDVFCGGSDWIPADSFTPRVTEEKY
RKWLEMMVDGYQIMIRIWGGGIWEEDIFYDICDELGVLVWQDFMFGCGNYPTFPSILQSI
KEECVANVGRLRHHPSVVIYAGNNEDYQVQEQCGLTYDYEDKDPESWLKTDFSARYIYEK
LLPEVVDAESPHVPYHPGSPWGDGLRTSDPTVGDMHQWNVWHGTQEKYQIFDTLGGRFNS
EFGMEAFPHIDTVRYYCTDPTQLYPQSHMLDFHNKADGHERRIATYLVENFRTKTDLEAF
IHLTQLSQAEALMFGYRGWRRQWGQDRHCGGALVWQLNDCWPVTSWSIVDYFLRKKPAYY
AMRRVLAPVAVAVKRAHFDWSVVHARVPKTSDYEVWVASQRQERVLATVELRFISIKSGK
EIKDKVVKKDIHLVANGTTDVFCGTIDNRNEEPHVLAVRIWIDGEVVSRDVDWPQPLKYL
DFEDRGLEVIPQGETITVRARKPTKGLVFEERAGVLVHDSAIDVVPGDEQVIKVRGLSKQ
DKPLRWTFLGKD

Enzyme Prediction     

EC	3.2.1.25:3

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	10	739	6e-93	0.699468085106383

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
225789	LacZ	1.11e-76	23	676	14	639	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
407658	Mannosidase_ig	2.74e-14	691	779	1	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
395572	Glyco_hydro_2	2.13e-10	212	314	2	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
236657	PRK10150	2.86e-06	28	445	19	417	beta-D-glucuronidase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRC92248.1|GH2	0.0	1	852	1	852
CBX99479.1|GH152|GH2	0.0	13	852	471	1312
CAE7030725.1|GH2	0.0	13	852	1	846
SMR45932.1|GH2	0.0	16	851	2	835
SMR47185.1|GH2	0.0	16	851	2	835

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5N6U_A	1.29e-143	10	781	15	753	Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_B Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_C Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_D Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12]
6BYE_A	1.45e-119	19	834	3	832	Crystal structure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri in complex with mannose [Xanthomonas citri pv. citri str. 306],6BYE_B Crystal structure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri in complex with mannose [Xanthomonas citri pv. citri str. 306]
6BYC_A	1.48e-119	19	834	3	832	Crystal structure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306]
2VJX_A	2.76e-119	24	747	11	719	Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	2.90e-119	24	747	13	721	Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|B8NW36|MANBB_ASPFN	0.0	18	851	3	844	Beta-mannosidase B OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=mndB PE=3 SV=1
sp|A2QYN2|MANBB_ASPNC	0.0	18	851	3	844	Beta-mannosidase B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=mndB PE=3 SV=1
sp|B0YBU9|MANBB_ASPFC	0.0	19	851	4	845	Beta-mannosidase B OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=mndB PE=3 SV=1
sp|Q2TXB7|MANBB_ASPOR	0.0	18	851	3	844	Beta-mannosidase B OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=mndB PE=3 SV=3
sp|Q0CCA0|MANBB_ASPTN	0.0	23	851	8	843	Beta-mannosidase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndB PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000060	0.000000

TMHMM  Annotations     

There is no transmembrane helices in QRC92248.1.