CAZyme Information: PWY91017.1

Basic Information

• Species: Aspergillus heteromorphus
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus heteromorphus
• CAZyme ID: PWY91017.1
• CAZy Family: GT32
• CAZyme Description: UDP-glucose:glyco protein glucosyltransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1490		166767.65	4.6930

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AheteromorphusCBS117.55	11436	1448321	306	11130

• Gene Location: location=MSFL01000002:1139145-1143855(-)

Full Sequence      

MVSGPGLVASWRLTFVLVASLLAGLGRTSPSVNVALQASFDAAPYLIELLETAAEENSTS
YFPLLDRIADGAFDDAVTDKELYDRFLEIVHEDGHLRTPESLSSFKVSLAIRSASPRITA
HYQFYNASVQHSLMAAQDAACPVWVHLDGKQYCSSAMERAQQDVMGSDEPRELPFDRVFG
DSSLPPAILYADVASPMFKDFHQSLSALAKEGQISYRVRYRHPQHWRSRPLFVSGYGVEL
ALKRTDYIVIDDRAAEQRSHDDVDSKTSDDVERDDLDDLKPLSSREVSKLGLNAASYVLE
SEDPFDTLLKISQDFPKYSSRIATHDISSELLQDIQASRLMLHPGMNTLWINGIQIYPKE
IDIFTLLDQVRRERNLIGKFRNLGLSAKEAVELLSHQILGEALVQDGPPRYNYRDEIEGG
GVIMWLNDLEKDARYESWPSELAGFMQRTHPGQLPAVRRDANNIVFPVDLSSAEDTDMVI
KTIQVFVKNKIPVRFGLIPVTFSDGATAQSKVAHYLQDTFGLASFMKYLETSASKNKLGS
PDKASFKAATEGLDVRPDKQSLSLEEVLKNEELAVLAERTSAYTSRLGMTEASHAFVNGA
PVTRDDNWPQEMSTRISRDTQLIQELIADGELEEETWLPSLFLDDASDRRNPELIPDNPK
DIRVVDLVNVADLRDSQGELLDQLPRLASNGKDALDSTHVIVIGDFDDESGHELLTAALG
SHTENSNAEILFLHNPKSEASSGSASTAIYRSLKENKGADAAQVLNDIANSDSPSHKEAQ
SIAHFWKAQQALVTEFGLSPGSRALIINGGVIGPIAKNTAVSSSDLDQLLVHEKSRRVTP
VAMATEELGLGNKISSPLAFAQLTSLTAPDAPNDFVKETSGFRLLLFDRLNDSRSTITVS
NSEDPTITILASIDPAAEKAQKWLPILKVLSELDGVRLRLVMNPREEIKELPTKRFYRYV
LGSEPSFNGDGSVSQPTASFSGVPVEALLTLGMDVPSTWLVAPKDSIHDLDNIKLSSVKD
GSDVDAIYALEHILIEGHSRDTTTNSPPRGVQLILGTENDPHFADTIIMANLGYFQFKAQ
PGLWNINLKPGRSERIFNIDSVGGQGYYPQPGDENTEVALLSFTGKPLYPRLSRKKGYET
EEVLETGPKPGSAMDYMSKGFNFASDILSSVGVGTKDGKQADINIFSVASGHLYERMLNI
MMVSVMRNAQNHSVKFWFIEQFLSPSFKSFLPHLAKEYKFSYEMVTYKWPHWLREQKEKQ
REIWGYKILFLDVLFPLDLDKVIFVDADQIVRTDMYDLVSLDLEGAPYGFTPMCDSRHEM
EGFRFWKQGYWKNFLRGQPYHISALYVVDLNRFRALAAGDRLRGQYQMLSADPESLSNLD
QDLPNHMQHYIPIKSLPQEWLWCETWCSDESQSQARTIDLCNNPMTKEPKLDRARRQVPE
WTEYDDEIAALSKKVDLELQQLRAEQEKEQVQELADQEDEESSGWDKDEL

Enzyme Prediction     

EC	2.4.1.-:29

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT24	1183	1431	9.4e-134	0.9959677419354839

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
408203	Glyco_transf_24	0.0	1183	1451	1	268	Glucosyltransferase 24. This is the catalytic domain found in UDP-glucose:glycoprotein glucosyltransferase (UGGT). This domain belongs to glucosyltransferase 24 family (GT24) A-type domain. The GT domain displays the expected glycosyltransferase type A (GT-A) fold.
133054	GT8_HUGT1_C_like	2.16e-180	1183	1431	1	248	The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity.
408201	Thioredoxin_14	6.39e-88	411	652	1	246	Thioredoxin-like domain. This is the third out of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).
399437	UDP-g_GGTase	1.85e-63	1036	1145	1	109	UDP-glucose:Glycoprotein Glucosyltransferase. UDP-g_GGTase is an important, central component of the QC system in the ER for checking that glycoproteins are folded correctly. This QC prevents incorrectly folded glycoproteins from leaving the ER.
408199	Thioredoxin_12	1.46e-62	42	229	1	185	Thioredoxin-like domain. This is one of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCR94558.1|GT24	0.0	1	1490	1	1495
BCS07152.1|GT24	0.0	1	1490	1	1495
CAK39488.1|GT24	0.0	1	1490	1	1495
CAS97678.1|GT24	0.0	1	1490	1	1495
BAE65008.1|GT24|2.4.1.-	0.0	1	1490	1	1487

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MU1_A	0.0	24	1455	3	1451	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5MZO_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TRF_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5NV4_A	0.0	24	1455	3	1451	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5Y7O_A	0.0	31	1151	5	1130	Crystal structure of folding sensor region of UGGT from Thermomyces dupontii [Thermomyces dupontii],5Y7O_B Crystal structure of folding sensor region of UGGT from Thermomyces dupontii [Thermomyces dupontii]
6TRT_A	0.0	24	1455	3	1451	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS2_A	0.0	24	1455	3	1217	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_C Chain C, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_D Chain D, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q09140|UGGG_SCHPO	1.85e-282	33	1451	23	1424	UDP-glucose:glycoprotein glucosyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gpt1 PE=1 SV=2
sp|Q6P5E4|UGGG1_MOUSE	1.78e-244	6	1451	20	1523	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Mus musculus OX=10090 GN=Uggt1 PE=1 SV=4
sp|Q9NYU2|UGGG1_HUMAN	1.14e-242	21	1451	36	1523	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Homo sapiens OX=9606 GN=UGGT1 PE=1 SV=3
sp|Q9JLA3|UGGG1_RAT	1.44e-242	29	1451	44	1523	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Rattus norvegicus OX=10116 GN=Uggt1 PE=1 SV=2
sp|Q09332|UGGG_DROME	1.72e-232	13	1456	9	1512	UDP-glucose:glycoprotein glucosyltransferase OS=Drosophila melanogaster OX=7227 GN=Uggt PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.001731	0.998215	CS pos: 28-29. Pr: 0.9325

TMHMM  Annotations     

There is no transmembrane helices in PWY91017.1.