CAZyme Information: PV10_02996-t45_1-p1

Basic Information

• Species: Exophiala mesophila
• Lineage: Ascomycota; Eurotiomycetes; ; Herpotrichiellaceae; Exophiala; Exophiala mesophila
• CAZyme ID: PV10_02996-t45_1-p1
• CAZy Family: GH12
• CAZyme Description: trehalose-phosphatase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
948		105749.93	6.1782

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_EmesophilaCBS40295	9237	N/A	56	9181

• Gene Location: location=KN847521:3363657-3366553(-)

Full Sequence      

MTVFIATLFLPYTVVFDLSSPRPASPNIQRPQSTNSASSKTKSPDTPAVSLFTPSAALTQ
SPGPLVQTPGATTDHEKIFLSQYSRPMHLRLPSHLKQDITQDEQSPPAPTLPPRSISQAD
LHSPGWGKSVTWNQPPSRAKSPPPSSILKHAAINNKEKAAAMRNNILATNIGQRQRKKTI
SHERRFSEYNYSVEKAQLGNGGLFMAINAAAEADLLEDKTWVGTLGCPTDALDNGLKESI
AEKLESAYDALAVYAADSDINGHYEHYCKTILWPVFHYQIPDHPKSKAYQDHSWIFYVKV
NEAFADRIAKNYKKGDIIWVHDYHLLLVPGLLRKRLPDAQIGFFLHTAFPSSEIFRCLSV
RSELLEGVLGSSMIALQNEEYCHHFLQTCSRLLNVEATRHGVLLEDGRFVHVQTCPIGVD
PKSLEEQRRLPEVAEWIRTISEKYQGKRIIVGRDKLDNIRGVRQKMLSYELFLNRNPQYE
DQVVLIQIATSSTEDPELSAAVADIVTRINSSHSTLAHQPVVFLKQDIDPAQYTALLTIA
DCLLITSLREGMNLTSHEFVFSQNPKFADTKCGPLILSEFTGSATVFGKSALLVNPWHYT
QCADTIKMALDMSDEERERRWKLMYKIVTENNAVKWYETFMEALDHAWREQSIRNSTSVP
RLSIAGTTAKYQKANRRLIILDYEGTLASWGAPTDIVLTTPKRSIDVLNDLTDDPKNIVY
VMSARTPEEMERLFSPVSDIGMIAENGAFLREPRTDEWIELADLDHAETWIGAVYKILQY
YVERIEGSRIELRHSSLVFDYSDAEDLSGAFKQAGECANHINDACRGQNVSAVPVEDGLL
ISEMDVNKGNAAAAIIENLESSVASEGLTFPDFLLVIGDSREDEYVFNWAHKLEKAGKIK
EVVTVTLGARSTEASATLTQGVTGVLTILEKLVRAKAEGQPPSNGAPK

Enzyme Prediction     

EC	2.4.1.15:11

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT20	188	645	2e-155	0.9305263157894736

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
340820	GT20_TPS	0.0	192	644	19	462	trehalose-6-phosphate synthase. Trehalose-6-Phosphate Synthase (TPS, EC 2.4.1.15) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain.
395781	Glyco_transf_20	0.0	201	644	31	468	Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex,.
184712	PRK14501	1.67e-169	201	935	28	726	putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional
223457	OtsA	2.69e-162	199	652	42	486	Trehalose-6-phosphate synthase [Carbohydrate transport and metabolism].
215556	PLN03064	2.00e-134	180	887	105	809	alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QMW41842.1|GT20	0.0	1	938	1	927
QRD86165.1|GT20	0.0	1	938	1	927
BAE60220.1|GT20	0.0	1	938	1	927
QMW29769.1|GT20	0.0	1	938	1	927
UDD61429.1|GT20	0.0	1	938	1	927

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5DXF_A	4.62e-97	182	647	48	527	Structure of Candida albicans trehalose-6-phosphate phosphatase N-terminal domain [Candida albicans SC5314],5DXF_B Structure of Candida albicans trehalose-6-phosphate phosphatase N-terminal domain [Candida albicans SC5314]
6JBI_A	8.94e-97	188	645	20	463	Structure of Tps1 apo structure [Pyricularia oryzae 70-15],6JBI_B Structure of Tps1 apo structure [Pyricularia oryzae 70-15],6JBR_A Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_B Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_D Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_F Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_H Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_K Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_M Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_O Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBW_A Structure of Tps1/UDP complex [Pyricularia oryzae 70-15],6JBW_B Structure of Tps1/UDP complex [Pyricularia oryzae 70-15]
5HVM_A	1.13e-91	198	645	37	475	Structure of Aspergillus fumigatus trehalose-6-phosphate synthase A in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293],5HVM_B Structure of Aspergillus fumigatus trehalose-6-phosphate synthase A in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293]
5HUT_A	7.92e-90	189	655	25	478	Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP-glucose [Candida albicans SC5314],5HUT_B Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP-glucose [Candida albicans SC5314],5HUU_A Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and glucose-6-phosphate [Candida albicans SC5314],5HUU_B Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and glucose-6-phosphate [Candida albicans SC5314],5HVL_A Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and validoxylamine A [Candida albicans SC5314],5HVL_B Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and validoxylamine A [Candida albicans SC5314]
5HUV_A	1.10e-89	189	655	25	478	Structure of Candida albicans trehalose-6-phosphate synthase E341R/E346R in complex with UDP-glucose [Candida albicans SC5314],5HUV_B Structure of Candida albicans trehalose-6-phosphate synthase E341R/E346R in complex with UDP-glucose [Candida albicans SC5314]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|O14081|TPSX_SCHPO	2.04e-198	135	935	139	944	Putative alpha,alpha-trehalose-phosphate synthase [UDP-forming] 106 kDa subunit OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC2E11.16c PE=1 SV=1
sp|Q9UUI7|TPSY_SCHPO	1.09e-178	124	932	103	889	Putative alpha,alpha-trehalose-phosphate synthase [UDP-forming] 100 kDa subunit OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC22F8.05 PE=1 SV=1
sp|P38426|TPS3_YEAST	1.94e-158	186	843	309	940	Trehalose synthase complex regulatory subunit TPS3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPS3 PE=1 SV=3
sp|P38427|TSL1_YEAST	2.10e-152	181	847	336	979	Trehalose synthase complex regulatory subunit TSL1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TSL1 PE=1 SV=1
sp|Q54K57|TPSA_DICDI	7.75e-120	197	880	40	691	Alpha,alpha-trehalose-phosphate synthase [UDP-forming] A OS=Dictyostelium discoideum OX=44689 GN=tpsA PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.874186	0.125817

TMHMM  Annotations     

There is no transmembrane helices in PV10_02996-t45_1-p1.