CAZyme Information: PV10_00065-t45_1-p1

Basic Information

• Species: Exophiala mesophila
• Lineage: Ascomycota; Eurotiomycetes; ; Herpotrichiellaceae; Exophiala; Exophiala mesophila
• CAZyme ID: PV10_00065-t45_1-p1
• CAZy Family: AA1
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1478		165130.10	4.8998

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_EmesophilaCBS40295	9237	N/A	56	9181

• Gene Location: location=KN847520:194832-199325(-)

Full Sequence      

MSSHSRTPQWSLALLGPLILSIFSTVAQSSAAVNVALKTSFDAAPYLVELLETAALENSS
SYFPLLDRIADGAFADLESDQDLYNHFIEIVQKDGHLSAPDSLSSFKFALSLHAAAPRVQ
AHFQYYNTSVQSLLPAAQDAACPVWVYLDGQQYCSPSLDRAQQPVQLPDVDTPLPFDRIL
GESSDGPPSILYADITHPLFAQFHHVVSKTARDGASSYRLRYRPPVSTSSNPLYVSGYGV
ELVLKRTDYIVIDDRDATTPDRKSPEQLSLEKAEEEELSDLKPLTSSEVAPLALNAASFI
ASSSNPLDTLLQISSDFPRYSSLLASANVSDDFLREHRYNRERFLPSGYNVLLINGMQIE
PRQINAYSLIDHLRRERRIIDNLKTIGLTSSEAIDLLSSQVIAESQANESPQRFDWRDNL
EDGKALMWLNDLETDKRYAGWPSSLQALFQRVYPGQLPQVARDIHNLVVPIDMNNIKDVE
LVVTALQSLVRRKVPLRIALVPIGDGETADTYAKVSYHILDTYGLTTLMAFYNDIVSGKK
LLSTPEQSFAAVIKGRTPREGKYVLTYDQALAADSVEQHLAARNTYIDRLALQGASIPFL
INGVVMTRTDSWFETMSARLYADLQILQQAVYGDLILEESWIPEFFIGQAPSRRNQLVFP
HDPKDVRILDVIALATAHGGDLAALPRVAGWDADLLGDRAHVLLVADLDSAAGRALLEET
IAVQHQRPHTDILILHNPAVEDASPGIACSLYAMVEDGRRDVYIEKLWDLLERKEDFAVL
DGAAVSACRTFWATKQPAARAIGFMAGETGLWVNGRTIGPLVAPFSTTDLESLLDSEREQ
RISPVTTAITGLGLEKRFEDAFDLAKVTSLVARSLKSDLPEGLRESAPLIRMDLFKVWNE
TYTAINLANHEDATVQVVAVMDPASEVTQQWVPIIQTLSQLHGINVKIFLNPKDRLTELP
VKRFLRHVVSSEPSFDDKGSLQRPMASFKGIPKDTLFNLGMIVPPSWLVAPEKSVHDLDN
IKLSSLAEGENVDAEYELEHILIEGHARDVTTSPAPRGVQLLLGTEQEPHFTDTIVMANL
GYFQFKANPGYWQIGLKPGRSSKIFNIDSVGPSGYEAAPGDETTSVALLSFQGLTLFPRL
SRKAGMEEEDVLESPTGALDYLSKGASFASSALSSLGFGKSTDSNADINIFSVASGHLYE
RMLNIMMVSVMKHTKHTVKFWFIEQFLSPDFKSTLPYLAEHYGFKYEMVTYKWPHWLRGQ
TEKQREIWGYKILFLDVLFPLDLDKVIFVDADQIVRTDMMELNRVDLKGAPYGFTPMCDS
RTEMEGFRFWKQGYWETYLRGKPYHISALYVVDLKRFRQVAAGDRLRQQYQALSADPNSL
SNLDQDLPNHMQHSLPIHSLSQEWLWCETWCSDESLAQAKTIDLCNNPQTKEPKLDRARR
QVPEWTEYDDEIARVIEAAKGVGSDGEAMDGKRTRDEL

Enzyme Prediction     

EC	2.4.1.-:29

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT24	1188	1435	4.8e-135	0.9959677419354839

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
133054	GT8_HUGT1_C_like	0.0	1188	1435	1	248	The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity.
408203	Glyco_transf_24	0.0	1188	1455	1	268	Glucosyltransferase 24. This is the catalytic domain found in UDP-glucose:glycoprotein glucosyltransferase (UGGT). This domain belongs to glucosyltransferase 24 family (GT24) A-type domain. The GT domain displays the expected glycosyltransferase type A (GT-A) fold.
408201	Thioredoxin_14	6.79e-84	414	658	1	248	Thioredoxin-like domain. This is the third out of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).
399437	UDP-g_GGTase	2.49e-64	1044	1153	1	109	UDP-glucose:Glycoprotein Glucosyltransferase. UDP-g_GGTase is an important, central component of the QC system in the ER for checking that glycoproteins are folded correctly. This QC prevents incorrectly folded glycoproteins from leaving the ER.
408199	Thioredoxin_12	9.39e-63	43	225	1	179	Thioredoxin-like domain. This is one of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UDD64319.1|GT24	0.0	10	1478	11	1487
BAE65008.1|GT24|2.4.1.-	0.0	1	1478	2	1487
QRD91624.1|GT24	0.0	1	1478	2	1487
QMW47293.1|GT24	0.0	1	1478	2	1487
QMW35231.1|GT24	0.0	1	1478	2	1487

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MU1_A	0.0	29	1455	7	1447	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5MZO_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TRF_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5NV4_A	0.0	29	1455	7	1447	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5Y7O_A	0.0	29	1154	2	1125	Crystal structure of folding sensor region of UGGT from Thermomyces dupontii [Thermomyces dupontii],5Y7O_B Crystal structure of folding sensor region of UGGT from Thermomyces dupontii [Thermomyces dupontii]
6TRT_A	0.0	29	1455	7	1447	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS2_A	0.0	29	1455	7	1213	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_C Chain C, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_D Chain D, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q09140|UGGG_SCHPO	1.30e-282	25	1478	14	1448	UDP-glucose:glycoprotein glucosyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gpt1 PE=1 SV=2
sp|Q9NYU2|UGGG1_HUMAN	8.97e-236	19	1455	33	1523	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Homo sapiens OX=9606 GN=UGGT1 PE=1 SV=3
sp|Q6P5E4|UGGG1_MOUSE	1.59e-235	22	1455	36	1523	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Mus musculus OX=10090 GN=Uggt1 PE=1 SV=4
sp|Q09332|UGGG_DROME	4.56e-233	45	1456	42	1508	UDP-glucose:glycoprotein glucosyltransferase OS=Drosophila melanogaster OX=7227 GN=Uggt PE=1 SV=2
sp|Q9JLA3|UGGG1_RAT	1.89e-232	14	1455	28	1523	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Rattus norvegicus OX=10116 GN=Uggt1 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.001671	0.998306	CS pos: 29-30. Pr: 0.9173

TMHMM  Annotations     

There is no transmembrane helices in PV10_00065-t45_1-p1.