CAZyme Information: PV07_06149-t44_1-p1

Basic Information

• Species: Cladophialophora immunda
• Lineage: Ascomycota; Eurotiomycetes; ; Herpotrichiellaceae; Cladophialophora; Cladophialophora immunda
• CAZyme ID: PV07_06149-t44_1-p1
• CAZy Family: GH2
• CAZyme Description: trehalose-phosphatase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
939		105772.20	6.7007

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_CimmundaCBS83496	12879	N/A	49	12830

• Gene Location: location=KN847042.1:5098524-5101395(+)

Full Sequence      

MTIFVATLFLPYTINFDVPASASRSESPELLRPQLALTQNTPSETQTVSLFTPGASSSPH
LSQLSQSSGPIPPTPGATTDHEKIFRPHNERKFSLTLPSLKKEVTVPVRAISRHDLHSPG
WGKTFTWNQPPSRAKSPPPSSILKHAVIGAKDVKEVKEKAISLAHNTGHKPGRRQRTRTV
SHERHFSHDSYTVEKAIIGNGGLYNAVDAAVDAGICEEKTWVGTLGCPVDALDEHLKESI
AEKLENDYDCLTVYTSDSDLNGHYEHYCKTILWPVVHYQIPDHPKSKAYEDHSWVFYAKV
NEAFADRIAKNWKKGDVIWIHDYHLLLLPGLLRQRLPEAQIGFFLHTAFPSSEIFRCLAV
RTDLLQGMLGANMIGFQNDEYGHHFLQTCSRLLNVEATKHGVILEDGRFVHVNTYPIGID
PKAIDLQRQVKEVKEWVHTITDKYRGKRIIVARDKLDSIRGVRQKILAYELFLNKYPEYR
DKVVLIQIATSSAEDPELSATVADIVTRVNSTHSTLAHQPLVFLKQDIDFSQYMALLTVA
ECLMITSLREGMNLTSHEWILCQDGKISQNKYGPLILSEFTGSATVFGHDALLVNPWHYS
HCADAIKQALDMSLEERENRWKALYDIVVHQNAVGWFEHYITGLEHAWKEHTVRDSTAVP
RLSLPSVKSKYQKAKKRLIILDYEGTLASWGSPTDIILTTPKRSVDVLNDLIEDKKNIVY
VMSSRMPEEMERLFSPVSGIGMIAENGAFVLDPGTDEWQELTNLEHVRNWKKGVKSILQY
YVERIEGSRVEERHASLIFDYSEAEDRQGAFKQAGECANHINDACRGQNVNAIPVEDALN
ISEMDINKGSAAQFIGENLEKLAQEKGVPFPDFLMVIGDSREDEYVFNWAHRLEKAEKIR
DVVTVTLGARSTEASATLTQGVTGVLSILEKLARTLQRV

Enzyme Prediction     

EC	2.4.1.15:11

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT20	176	645	4.5e-157	0.968421052631579

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
340820	GT20_TPS	0.0	199	645	26	463	trehalose-6-phosphate synthase. Trehalose-6-Phosphate Synthase (TPS, EC 2.4.1.15) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain.
395781	Glyco_transf_20	6.85e-178	201	645	31	469	Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex,.
184712	PRK14501	6.19e-153	201	934	28	725	putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional
223457	OtsA	3.62e-151	221	650	64	484	Trehalose-6-phosphate synthase [Carbohydrate transport and metabolism].
215555	PLN03063	1.83e-127	172	887	8	719	alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCS07788.1|GT20	0.0	1	935	1	922
BCR95224.1|GT20	0.0	1	935	1	922
QMW41842.1|GT20	0.0	1	933	1	922
QRD86165.1|GT20	0.0	1	933	1	922
BAE60220.1|GT20	0.0	1	933	1	922

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6JBI_A	2.97e-95	240	644	61	462	Structure of Tps1 apo structure [Pyricularia oryzae 70-15],6JBI_B Structure of Tps1 apo structure [Pyricularia oryzae 70-15],6JBR_A Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_B Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_D Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_F Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_H Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_K Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_M Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_O Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBW_A Structure of Tps1/UDP complex [Pyricularia oryzae 70-15],6JBW_B Structure of Tps1/UDP complex [Pyricularia oryzae 70-15]
5DXF_A	3.02e-94	262	652	146	532	Structure of Candida albicans trehalose-6-phosphate phosphatase N-terminal domain [Candida albicans SC5314],5DXF_B Structure of Candida albicans trehalose-6-phosphate phosphatase N-terminal domain [Candida albicans SC5314]
5HUT_A	9.49e-90	198	655	29	478	Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP-glucose [Candida albicans SC5314],5HUT_B Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP-glucose [Candida albicans SC5314],5HUU_A Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and glucose-6-phosphate [Candida albicans SC5314],5HUU_B Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and glucose-6-phosphate [Candida albicans SC5314],5HVL_A Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and validoxylamine A [Candida albicans SC5314],5HVL_B Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and validoxylamine A [Candida albicans SC5314]
5HUV_A	9.49e-90	198	655	29	478	Structure of Candida albicans trehalose-6-phosphate synthase E341R/E346R in complex with UDP-glucose [Candida albicans SC5314],5HUV_B Structure of Candida albicans trehalose-6-phosphate synthase E341R/E346R in complex with UDP-glucose [Candida albicans SC5314]
5HVM_A	5.22e-89	198	644	37	474	Structure of Aspergillus fumigatus trehalose-6-phosphate synthase A in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293],5HVM_B Structure of Aspergillus fumigatus trehalose-6-phosphate synthase A in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|O14081|TPSX_SCHPO	1.35e-196	130	931	139	940	Putative alpha,alpha-trehalose-phosphate synthase [UDP-forming] 106 kDa subunit OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC2E11.16c PE=1 SV=1
sp|Q9UUI7|TPSY_SCHPO	5.43e-187	4	932	5	889	Putative alpha,alpha-trehalose-phosphate synthase [UDP-forming] 100 kDa subunit OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC22F8.05 PE=1 SV=1
sp|P38427|TSL1_YEAST	7.72e-160	186	855	341	986	Trehalose synthase complex regulatory subunit TSL1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TSL1 PE=1 SV=1
sp|P38426|TPS3_YEAST	3.39e-156	186	855	309	951	Trehalose synthase complex regulatory subunit TPS3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPS3 PE=1 SV=3
sp|Q54NU9|TPSB_DICDI	8.84e-123	217	903	94	774	Alpha,alpha-trehalose-phosphate synthase [UDP-forming] B OS=Dictyostelium discoideum OX=44689 GN=tpsB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.907664	0.092347

TMHMM  Annotations     

There is no transmembrane helices in PV07_06149-t44_1-p1.