CAZyme Information: PUG3_T012268-RA-p1

Basic Information

• Species: Globisporangium ultimum
• Lineage: Oomycota; NA; ; Pythiaceae; Globisporangium; Globisporangium ultimum
• CAZyme ID: PUG3_T012268-RA-p1
• CAZy Family: GT22
• CAZyme Description: Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
318		35716.98	9.3938

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_GultimumBR650	14086	1223559	0	14086

• Gene Location: location=PultBR650_SC2114:2327-3614(-)

Full Sequence      

MLLGLLILSATKFRQKKDLQGAFLYAVLLMLKHIYLYVALLYFASSENEGSGDDEEETNR
RKRSISNTDMHETIQNFYDGKATHPDPIAGLKQIFSRLFPVQRGLCHAYWAPNVWALYAF
ADKLLVLVGFPGATAGVALMSGGLVQEASFAILPSIPPIVVWKYPDSSLFMSALVYCMMC
SFLFGYHVHEKAILQSVNDMRLYRFASIISTISLFPLLFTSAEHGTKVLLAITHAVLAYV
TLDPFIKHSLKTRHIKANAVSMSGFEKLYLFALYPFVPLILFSVSCAVGNLYVWGMALSQ
HYRKLSAVKSYLHAHKWP

Enzyme Prediction     

EC	2.4.1.265:13	2.4.1.-:9

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT57	1	295	5.5e-47	0.6923076923076923

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397324	Alg6_Alg8	4.28e-67	1	294	147	472	ALG6, ALG8 glycosyltransferase family. N-linked (asparagine-linked) glycosylation of proteins is mediated by a highly conserved pathway in eukaryotes, in which a lipid (dolichol phosphate)-linked oligosaccharide is assembled at the endoplasmic reticulum membrane prior to the transfer of the oligosaccharide moiety to the target asparagine residues. This oligosaccharide is composed of Glc(3)Man(9)GlcNAc(2). The addition of the three glucose residues is the final series of steps in the synthesis of the oligosaccharide precursor. Alg6 transfers the first glucose residue, and Alg8 transfers the second one. In the human alg6 gene, a C->T transition, which causes Ala333 to be replaced with Val, has been identified as the cause of a congenital disorder of glycosylation, designated as type Ic OMIM:603147.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UIZ29794.1|GT57	2.16e-110	1	316	173	572
CCA20034.1|GT57	2.13e-63	1	293	159	495
AFJ50184.1|GT57	3.36e-45	2	294	169	506
AER94052.1|GT57	1.13e-41	2	294	136	474
NP_950200.2|GT57	5.04e-41	4	294	171	507

PDB Hits     

PUG3_T012268-RA-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q6P8H8|ALG8_MOUSE	8.95e-42	4	294	171	507	Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Mus musculus OX=10090 GN=Alg8 PE=2 SV=2
sp|Q0P5D9|ALG8_BOVIN	4.60e-41	4	294	171	507	Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Bos taurus OX=9913 GN=ALG8 PE=2 SV=1
sp|Q9BVK2|ALG8_HUMAN	8.85e-41	2	294	169	507	Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Homo sapiens OX=9606 GN=ALG8 PE=1 SV=2
sp|O80505|ALG8_ARATH	2.43e-38	2	294	159	497	Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Arabidopsis thaliana OX=3702 GN=At2g44660 PE=2 SV=3
sp|Q5AWM9|ALG8_EMENI	1.10e-33	2	243	157	427	Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=alg8 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000004	0.000024

TMHMM  Annotations     

Start	End
21	43
167	189
202	219
272	294