CAZyme Information: PTTG_12539-t43_1-p1

Basic Information

• Species: Puccinia triticina
• Lineage: Basidiomycota; Pucciniomycetes; ; Pucciniaceae; Puccinia; Puccinia triticina
• CAZyme ID: PTTG_12539-t43_1-p1
• CAZy Family: GT20
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1064	ADAS02000016.1|CGC1	118618.92	6.6473

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Ptriticina1-1BBBDRace1	15692	630390	814	14878

• Gene Location: location=ADAS02000016.1:597959-601222(-)

Full Sequence      

MEVSENEFIPFPGPSGPAAPLMTTRPPLLLVWLLIAHSLCSGSPESYTNSTDIVYPTRFR
GVEWDNEQWKLSTTLLEPGRYQSRISLANGYLGINLAALGPFFEKDNQSLNGWPVLDRRQ
SFATIAGFYDSQPKLNSSNFPWLDQYGGESIISGVPHWAGLLVKTEAATLDASTDPRSIK
NFHSALDLRTGLMKWKFEWVIDGQPEISIEYTMFVHKLHVNRAAVQLELKAVSDFELSVI
DLLDGDCAARTDFVEKRYHSSSSTICSAVRPNGMANVTGHVCSSLRSEAFSQSYLLKGSP
TPSSSASPSTIAQSARIKLKREQTVAVQKFIGAASGDAFSDPHTVALQSAIAGAKDGFSS
QLDSHRREWSSIMTKESVDDYALAQYDPSTIDPNIVELQILAITNPFHLLQNTISSNAIA
LANTSSALDNWSISVCGLGSSCYGGMIFWDAEVWMAPGLVLSHPHAAQRIINYRVAQFPQ
AQDNIKMAFSSSQNRTNQFSPDDAIYPWTSGRFGNCTASGPCFDYEYHINGDIGLSFFHH
LVVTGNFEYFKRHLWPIYQAIAGLFSNLLSETSTHKYALRNATDPDEYANHVDNPAYTMF
LIKHHLTTANELRGRVGQKPKPTWLKQANNLALPIDDQSGIILEYEGMNSSIVVKQADVV
LIDDFLDFPNPHRLENLEYYGTKQSLNGPGMTYGVYSVVENRFQISGCSSYTYHLFSSQP
YIRAPWFQFSEQLVDDYSQNGGIHPAFPFLTGMGGDYRVTVYGYLGLRLELDHLSVDPSL
PPQIASLSYRKIYWHGYPIRAKSNQTHTSLFRPPSGALADADPDYAEAPIPVKHRSSGRI
LKLGRARTVTVPNRPVYLANPIQCAPIKSDQAFLPGQFPLSILDGSSATRWIPSDLPATV
TVPLNEHFRAKQIIGFGFQSSNFTDFRIRFFDDPGQRTALSTYSSFNGTHVGFHPFVGLD
DHDDHDHQQKISQKDHGWSFYRFDQPIGFSKYANLTVFDTDQIQSAHPESFHTQDSRPKN
QHTSQRNSKSLGMAQWTLLTSHHHSSRTPESHNRNSGQTLIELL

Enzyme Prediction     

EC	3.2.1.28:5

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH65	407	770	3.9e-72	0.9811827956989247

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
224471	ATH1	1.40e-69	67	793	9	724	Trehalose and maltose hydrolase (possible phosphorylase) [Carbohydrate transport and metabolism].
397616	Glyco_hydro_65N	1.77e-36	79	335	11	239	Glycosyl hydrolase family 65, N-terminal domain. This family of glycosyl hydrolases contains vacuolar acid trehalase and maltose phosphorylase.Maltose phosphorylase (MP) is a dimeric enzyme that catalyzes the conversion of maltose and inorganic phosphate into beta-D-glucose-1-phosphate and glucose. This domain is believed to be essential for catalytic activity although its precise function remains unknown.
281612	Glyco_hydro_65m	6.53e-27	405	774	3	387	Glycosyl hydrolase family 65 central catalytic domain. This family of glycosyl hydrolases contains vacuolar acid trehalase and maltose phosphorylase.Maltose phosphorylase (MP) is a dimeric enzyme that catalyzes the conversion of maltose and inorganic phosphate into beta-D-glucose-1-phosphate and glucose. The central domain is the catalytic domain, which binds a phosphate ion that is proximal the the highly conserved Glu. The arrangement of the phosphate and the glutamate is thought to cause nucleophilic attack on the anomeric carbon atom. The catalytic domain also forms the majority of the dimerization interface.
237517	PRK13807	4.25e-13	67	793	8	713	maltose phosphorylase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QIW95062.1|GH65	0.0	55	933	23	913
SMY22156.1|GH65	3.35e-316	47	930	10	905
SMR48299.1|GH65	2.70e-315	47	947	10	924
AQA29363.1|GH65	9.06e-315	51	930	16	906
UJO19712.1|GH65	2.57e-314	51	930	16	906

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7FE3_A	2.54e-35	67	770	22	643	Chain A, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE3_B Chain B, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE3_C Chain C, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE4_A Chain A, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE4_B Chain B, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE4_C Chain C, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101]
3WIQ_A	1.13e-21	405	796	310	722	Crystal structure of kojibiose phosphorylase complexed with kojibiose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_A Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_B Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_C Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_D Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|D4B5H9|ATC1_ARTBC	3.34e-255	55	918	53	932	Cell wall acid trehalase ARB_03719 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_03719 PE=1 SV=1
sp|P78617|TREA_EMENI	1.27e-242	47	918	45	926	Acid trehalase OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=treA PE=3 SV=1
sp|M1RNJ8|ATH1_CANGB	2.28e-101	59	922	157	1036	Periplasmic/secreted acid trehalase ATH1 OS=Candida glabrata OX=5478 GN=ATH1 PE=1 SV=1
sp|Q5AAU5|ATC1_CANAL	5.74e-97	28	810	45	843	Cell wall acid trehalase ATC1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=ATC1 PE=1 SV=2
sp|N1P212|ATH1_YEASC	7.49e-94	59	779	121	837	Periplasmic acid trehalase ATC1 OS=Saccharomyces cerevisiae (strain CEN.PK113-7D) OX=889517 GN=CENPK1137D_1727 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.256371	0.743627	CS pos: 42-43. Pr: 0.6540

TMHMM  Annotations     

There is no transmembrane helices in PTTG_12539-t43_1-p1.