CAZyme Information: PTTG_06227-t43_1-p1

Basic Information

• Species: Puccinia triticina
• Lineage: Basidiomycota; Pucciniomycetes; ; Pucciniaceae; Puccinia; Puccinia triticina
• CAZyme ID: PTTG_06227-t43_1-p1
• CAZy Family: GH5
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1002		113027.45	6.7059

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Ptriticina1-1BBBDRace1	15692	630390	814	14878

• Gene Location: location=ADAS02000036.1:643301-647850(+)

Full Sequence      

MYHLFSSLLCFIGLSALPFSVSAAASERGPPLPSTHLSLSSFEWSLVNANRSIVVPTPFL
NQPHLALIKAGVIDDPNIGLNEGTTRWVGEEEAWTWETKFKVKTDDQWAKAERFYLVFNG
LDTFCDIQVNGQRIGSTDNAFRSWVFDATQVIKSSQNQTIPLSLRFASAYATASRIAHEP
GNMWFPNKSADKKNSTTLQPFEYNYRNWARKQQSDFGWDWGPAYLPSGPIKPAYLIGLSA
PVQSSLGLIDQGKAHQPQTRTVHGKQSVTPGTTPKGLSLLAGKPTDKKRRRATPETTGTP
SLWVHRTIIDIYRSGQRNNLMPDQGAPWVINITLPITSPTLVNATLSGNLWPRLFTHSRI
HHRSKQVELWYPATLGSPTLYDLELKLQVDAHDASLGGEDAELSWWEKVGFRTIVVDQSR
YTDQEVANGIRPGTRFTFIINGKPFYVQGSSMIPIDNFAARANSSTIRWILESAVLAHQN
VVRIWGGGAYQTDEFYDLCDELGILAWSESVFACGAYPLSPESFLDNIRAEVSENVARLN
RHPSTALWAGNNEGEGYLIDVNRTWTNGSIYFNQYDHLNNRVLRELVLDNTRSISYIPSS
TTQGYLSLDPYVARYYNSTPGELYGDKEHYNYDTSVSFNISSYPVGRFVNEFGFHSMPSI
YTWDRILKSPEDYEFNSTVIRAHNKHNPAESLTYPWPADDGQKQMTTGVTDHYPTPNITG
DHHTQLAQWAYSTQVFQAAFMASQIHYYRLGASRGENNMGAVYWQLNDVWEGISWSSIEY
TGRWKVFHYIAARVQSHVVISPIFHQTNNTLDIYVTSDLWQPIEGTAQWTWYDFTGQQLS
DTTTKSFSVEPIHSTQLYHAGGLSEMVPSGHAPNNAWMHLTLTTSDGKYTNEQFFHPVEL
KDCQLQPTKVETRPIGHNQVSVEVASGGVALWVNVEHPPGVRGYFKDMETGLPSNAFFLR
PKETRRLEFVLKGKDSDHDGLAKNLASRMIVRTLWDNQHLSQ

Enzyme Prediction     

EC	3.2.1.25:4

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	70	858	1.1e-66	0.6409574468085106

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
225789	LacZ	1.72e-40	94	794	65	628	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
407658	Mannosidase_ig	5.18e-16	809	902	1	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
407625	Ig_mannosidase	1.40e-10	917	996	12	78	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
397119	Glyco_hydro_2_C	2.39e-04	438	620	8	185	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
397120	Glyco_hydro_2_N	0.009	40	184	21	154	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCS20939.1|GH2	3.87e-192	37	998	23	922
BCS16350.1|GH2	1.46e-190	21	998	5	929
BCS04781.1|GH2	1.46e-190	21	998	5	929
CBF85479.1|GH2	2.61e-190	36	998	23	925
EAA64028.1|GH2	2.61e-190	36	998	23	925

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4CVU_A	2.02e-175	12	996	7	938	Structure of Fungal beta-mannosidase from Glycoside Hydrolase Family 2 of Trichoderma harzianum [Trichoderma harzianum],4UOJ_A Chain A, BETA-MANNOSIDASE GH2 [Trichoderma harzianum],4UOJ_B Chain B, BETA-MANNOSIDASE GH2 [Trichoderma harzianum]
6DDT_A	3.24e-91	41	819	15	699	mouse beta-mannosidase (MANBA) [Mus musculus],6DDU_A mouse beta-mannosidase bound to beta-D-mannose (MANBA) [Mus musculus]
2VJX_A	1.76e-68	44	910	15	764	Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	1.81e-68	44	910	17	766	Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	1.83e-68	44	910	17	766	Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q0CI48|MANBA_ASPTN	1.85e-191	20	996	8	927	Beta-mannosidase A OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndA PE=3 SV=2
sp|Q5BCI8|MANBA_EMENI	4.63e-191	36	998	23	925	Beta-mannosidase A OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mndA PE=3 SV=1
sp|A2QWU9|MANBA_ASPNC	8.52e-189	20	998	4	928	Beta-mannosidase A OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=mndA PE=3 SV=1
sp|Q4WMS9|MANBA_ASPFU	9.10e-183	20	998	7	923	Beta-mannosidase A OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndA PE=3 SV=1
sp|B0Y7S2|MANBA_ASPFC	3.57e-182	20	998	7	923	Beta-mannosidase A OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=mndA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000283	0.999695	CS pos: 23-24. Pr: 0.9773

TMHMM  Annotations     

There is no transmembrane helices in PTTG_06227-t43_1-p1.