CAZyme Information: PTTG_05863-t43_2-p1

Basic Information

• Species: Puccinia triticina
• Lineage: Basidiomycota; Pucciniomycetes; ; Pucciniaceae; Puccinia; Puccinia triticina
• CAZyme ID: PTTG_05863-t43_2-p1
• CAZy Family: GH37
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
715		81111.91	7.1465

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Ptriticina1-1BBBDRace1	15692	630390	814	14878

• Gene Location: location=ADAS02000063.1:166516-169473(+)

Full Sequence      

MTTSDQETRHRSSVSLDGRYFIDSESHQVISLRGVSLSGCSKLPSKPDGRTHQAELFFEH
RQVSFVDHPLKLEHAPHYLAQLVRWGFNLIRLVICWEALEHAGPGIYDLEYIQYITELVN
LCQQHGLKVLVDAHQDVWSRFSGGSGAPGWTLELAGFEITNLSETGAAALQQLGAPKGVW
PSGYQKLAAGTMFTLFFAGDTFAPNRKVKRNLHRQWAEETTGEELITLQQFLQGSMVEAF
GQLADSLSSFECVIGFEPMNEPHRGFINLYSPYQWNPMTDLFIRDCPSFLEAVALGDGHS
QRIDVYTPTWPIPSFRFHTRRITPHVRAWQSSVECIWKEHGVWRWDEKRRKPIVLKPKHF
NLDPATGKPFDFYSQALYPFVSRFAARVQSHRHEWIIPVGPIPNEFYPKWDHSQRPQNLV
AGPHFYDLFSLVHKSHGTLTMDVQGICMNKPIWKWMHFGHVAARKNYTEQIKNIVDSVYK
NIGEIPCMIGELGICMDLNNGESFKTGNFYWQHHQVNALLAACESNMVSFVLWNFNPYNT
DEYGDGWNGENFSFISQSEGDGSSPHSQARILSAIVRPSAQKVAGIPLRSTYDSEQSTFD
FEYKSPEAGLESKQASLLKTTRTEIFLPSERFPANQIKISVSDGTYSHEVNNQLLIWNHS
NLEPGARHIIKITSPVRVKVDKPDGTRRVSGFTLVLLLSLFVGLLALWSGGSKSL

Enzyme Prediction     

EC	3.2.1.45:1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH5	26	540	1.4e-165	0.9925788497217068

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
408348	Glyco_hydro_5_C	5.82e-19	577	672	1	86	Glycoside hydrolase family 5 C-terminal domain. This is the C-terminal domain of endo-glycoceramidase II (EGC), a membrane-associated family 5 glycosidase pfam00150. The C-terminal domain assumes a beta-sandwich fold, which resembles that of many carbohydrate-binding modules.
395098	Cellulase	2.32e-08	78	136	29	88	Cellulase (glycosyl hydrolase family 5).
225344	BglC	1.65e-07	78	167	78	167	Aryl-phospho-beta-D-glucosidase BglC, GH1 family [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ULM60693.1|GH5_12	8.22e-182	11	672	69	813
UPL04364.1|GH5_12	2.97e-174	14	689	19	756
CDR48502.1|GH5_12	4.36e-174	15	673	40	783
QGI82100.1|GH5_12	4.39e-174	14	655	18	713
QGI64843.1|GH5_12	4.39e-174	14	655	18	713

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7LPO_A	3.83e-73	20	570	27	608	Chain A, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPO_B Chain B, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPO_C Chain C, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPO_D Chain D, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPP_A Chain A, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPP_B Chain B, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPP_C Chain C, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPP_D Chain D, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPQ_A Chain A, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPQ_B Chain B, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPQ_C Chain C, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPQ_D Chain D, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99]
2OSW_A	2.77e-10	12	284	28	246	Endo-glycoceramidase II from Rhodococcus sp. [Rhodococcus sp.],2OSW_B Endo-glycoceramidase II from Rhodococcus sp. [Rhodococcus sp.],2OYK_A Endo-glycoceramidase II from Rhodococcus sp.: cellobiose-like isofagomine complex [Rhodococcus sp.],2OYK_B Endo-glycoceramidase II from Rhodococcus sp.: cellobiose-like isofagomine complex [Rhodococcus sp.],2OYL_A Endo-glycoceramidase II from Rhodococcus sp.: cellobiose-like imidazole complex [Rhodococcus sp.],2OYL_B Endo-glycoceramidase II from Rhodococcus sp.: cellobiose-like imidazole complex [Rhodococcus sp.],2OYM_A Endo-glycoceramidase II from Rhodococcus sp.: five-membered iminocyclitol complex [Rhodococcus sp.],2OYM_B Endo-glycoceramidase II from Rhodococcus sp.: five-membered iminocyclitol complex [Rhodococcus sp.]
2OSX_A	2.77e-10	12	284	28	246	Chain A, Endoglycoceramidase II [Rhodococcus sp.]
2OSY_A	1.48e-09	12	159	28	163	Chain A, Endoglycoceramidase II [Rhodococcus sp.],2OSY_B Chain B, Endoglycoceramidase II [Rhodococcus sp.]
5CCU_A	6.87e-07	75	153	81	158	Chain A, Putative secreted endoglycosylceramidase [Rhodococcus hoagii 103S],5CCU_B Chain B, Putative secreted endoglycosylceramidase [Rhodococcus hoagii 103S]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|I1BTD7|EGCR1_RHIO9	5.39e-163	18	664	19	660	Glucosylceramidase OS=Rhizopus delemar (strain RA 99-880 / ATCC MYA-4621 / FGSC 9543 / NRRL 43880) OX=246409 GN=ERC1 PE=1 SV=1
sp|H1AE12|EGCR1_CRYNH	1.38e-154	12	648	14	655	Glucosylceramidase OS=Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) OX=235443 GN=EGC1 PE=1 SV=1
sp|H1AE13|EGCR1_ASPFM	1.55e-87	16	691	8	742	Glucosylceramidase OS=Neosartorya fumigata OX=746128 GN=egc1 PE=1 SV=1
sp|P40566|EGH1_YEAST	1.29e-79	14	669	7	736	Ergosteryl-beta-glucosidase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=EGH1 PE=1 SV=1
sp|A0A3S5YBC7|EGCSE_RHOH1	3.49e-06	75	153	73	150	Endoglycoceramidase I OS=Rhodococcus hoagii (strain 103S) OX=685727 GN=REQ_38260 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.999820	0.000228

TMHMM  Annotations     

Start	End
689	711