CAZyme Information: PSURA_72878T0-p1

Basic Information

• Species: Phytophthora ramorum
• Lineage: Oomycota; NA; ; Peronosporaceae; Phytophthora; Phytophthora ramorum
• CAZyme ID: PSURA_72878T0-p1
• CAZy Family: CE2
• CAZyme Description: Oligosaccharyltransferase, STT3 subunit

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
884		97890.46	7.1268

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PramorumPr-102	15743	N/A	251	15492

• Gene Location: location=PramPr-102_SC0001:1002203-1004937(+)

Full Sequence      

MAPKSKSKKTASASSDDSENEAPVVEPTSAKAVDPTRVVFGVDLDDPRDAEDDENALAPV
AKFAILAMICLMSFSIRLFAVVRYESVIHEFDPYFNFRTTKYLASEGFLEFLDWFDDRAW
YPLGRIIGGTIYPGLMYTAALVYWVLNLLNISINVRNTCVFLAPLFAANTAVASYLLTKE
VTKRSSAGLLAAAFAGIVPSYISRSVGGSYDNEGVAIFALIFVFYLWIKAVHTGSMFWSA
ACALTYFYMVAAWGGYVFIINMIPIYVLVMIFAGRYTPRLYIAYSTFYTLGSLMAMQVPF
VGFNVIKQAECAGSHGVFVLLQVYCFVNWLRNYISAASFRRLVLVGAGLLVSGFALALLV
LQLMGKVQWTGRSLTLLDPTYASKYIPIIASVSEHQPTTWTSYFFDLHILVPFAPVGLYF
LFQNLTDGGIFVILYGTVAWYFAGVMVRLMLTLAPIACILGAVGISSTLRKFMGILARPS
TFVVQKDGVQQVPKILALGVVSGLLLMLLSYSLHATYVSSMAYSSPSIVIEAGRTQTGER
VNFDDYREAYFWLRQNTDANAKIMSWWDYGYQMSAMANRTVIVDNNTWNNTHIATVGRAL
ASSEEDAYPILQSLDVDYVLVIFGGVTGYSSDDINKFLWPVRIGSGVYPDDMPAERDYYS
ASGNFDVGPGGSKVLHNCLAYKLCYYRFGEVMTDYQRPPGYDRARNTEVGVKNIKLTHLE
EAFTSEHWIVRIFKVKKQPNVEPTTAVKKARLSEASAQEKDISKEKTRFLGCATGEDTLG
NDRVYAGGPSGANFNLALHHAKEKGKRYFAVARVAGEGHAFAFNKLPVNEADLDGNDEGC
MRPCKDSNSHSCGCADAGCTDMNVSPGKGQEHNRRWAIYERESN

Enzyme Prediction     

EC	2.4.99.18:21

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT66	63	696	9.9e-171	0.8585858585858586

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
396873	STT3	4.79e-111	60	524	1	478	Oligosaccharyl transferase STT3 subunit. This family consists of the oligosaccharyl transferase STT3 subunit and related proteins. The STT3 subunit is part of the oligosaccharyl transferase (OTase) complex of proteins and is required for its activity. In eukaryotes, OTase transfers a lipid-linked core-oligosaccharide to selected asparagine residues in the ER. In the archaea STT3 occurs alone, rather than in an OTase complex, and is required for N-glycosylation of asparagines.
224206	Stt3	4.08e-66	59	737	17	731	Asparagine N-glycosylation enzyme, membrane subunit Stt3 [Posttranslational modification, protein turnover, chaperones].
275016	archaeo_STT3	2.13e-18	63	621	1	586	oligosaccharyl transferase, archaeosortase A system-associated. Members of this protein family occur, one to three members per genome, in the same species of Euryarchaeota as contain the predicted protein-sorting enzyme archaeosortase (TIGR04125) and its cognate protein-sorting signal PGF-CTERM (TIGR04126).

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UIZ23018.1|GT66	0.0	1	881	1	882
CCA15036.1|GT66	0.0	51	879	51	877
CCA23157.1|GT66	0.0	51	879	51	877
AFJ69334.1|GT66	0.0	51	880	53	890
ACI33169.1|GT66	2.69e-276	62	740	19	699

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6FTG_5	8.60e-276	62	740	19	699	Subtomogram average of OST-containing ribosome-translocon complexes from canine rough microsomal membranes [Canis lupus familiaris],6FTI_5 Cryo-EM Structure of the Mammalian Oligosaccharyltransferase Bound to Sec61 and the Programmed 80S Ribosome [Canis lupus familiaris]
6FTJ_5	1.26e-275	62	736	19	695	Cryo-EM Structure of the Mammalian Oligosaccharyltransferase Bound to Sec61 and the Non-programmed 80S Ribosome [Canis lupus familiaris]
6S7O_A	2.45e-275	62	740	19	699	Cryo-EM structure of human oligosaccharyltransferase complex OST-A [Homo sapiens]
6S7T_A	6.36e-268	62	768	73	803	Cryo-EM structure of human oligosaccharyltransferase complex OST-B [Homo sapiens]
6C26_A	2.81e-213	60	737	15	684	The Cryo-EM structure of a eukaryotic oligosaccharyl transferase complex [Saccharomyces cerevisiae S288C],6EZN_F Cryo-EM structure of the yeast oligosaccharyltransferase (OST) complex [Saccharomyces cerevisiae S288C],7OCI_F Chain F, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 [Saccharomyces cerevisiae S288C]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|F1PJP5|STT3A_CANLF	2.20e-275	62	740	19	699	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A OS=Canis lupus familiaris OX=9615 GN=STT3A PE=1 SV=3
sp|P46978|STT3A_MOUSE	2.20e-275	62	740	19	699	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A OS=Mus musculus OX=10090 GN=Stt3a PE=1 SV=1
sp|P46977|STT3A_HUMAN	1.26e-274	62	740	19	699	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A OS=Homo sapiens OX=9606 GN=STT3A PE=1 SV=2
sp|Q5RCE2|STT3A_PONAB	1.26e-274	62	740	19	699	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A OS=Pongo abelii OX=9601 GN=STT3A PE=2 SV=1
sp|Q2KJI2|STT3A_BOVIN	1.26e-274	62	740	19	699	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A OS=Bos taurus OX=9913 GN=STT3A PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.999520	0.000483

TMHMM  Annotations     

Start	End
63	82
124	146
159	178
188	207
214	236
251	273
280	302
312	330
342	364
400	422
429	447
452	474
495	517