CAZyme Information: PSK78031.1

Basic Information

• Species: [Candida] auris
• Lineage: Ascomycota; Saccharomycetes; ; Debaryomycetaceae; Candida; [Candida] auris
• CAZyme ID: PSK78031.1
• CAZy Family: GT2
• CAZyme Description: unspecified product

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
345		38784.34	5.2916

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_CaurisB11243	5595	N/A	158	5437

• Gene Location: location=PYGM01000023:6291-7328(-)

Full Sequence      

MSKLRLFWILSAAALTVSSSPCNPTKDKFCVAQNDALSTAVFEPLTKAPTHLSSNVAAPS
ITFGAEGAKMRIFERGENPQLFSTSYILYGRAEAQIRASHGKGVISSMYMQSDDLDEIDV
AEFFGGEPHKFQTNYFVKGNISNYDRDRYHNVYDDLTQEWHTFGVEWTPEAMFWYYNGQI
VRRVRSAHNTQGFPSSPMQLKFSLWVGGEPKNHPGTIEWAGGEADWKELPYEMGIRNVRL
VDYSSGEKYVYGSGQDLTAHRGQVFGKKKRDSSQEKGEIGNGIGSVVDDEKEEMCDDERV
LEKVPKGGSEEKTEEVFEGSGSRVQSGFRVSFVVSFMIYLFQNVL

Enzyme Prediction     

No EC number prediction in PSK78031.1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	66	222	1e-57	0.9873417721518988

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
185692	GH16_fungal_CRH1_transglycosylase	2.51e-85	55	244	13	203	glycosylphosphatidylinositol-glucanosyltransferase. Group of fungal GH16 members related to Saccharomyces cerevisiae Crh1p. Chr1p and Crh2p are transglycosylases that are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan, an important step in the assembly of new cell wall. Both have been shown to be glycosylphosphatidylinositol (GPI)-anchored. A third homologous protein, Crr1p, functions in the formation of the spore wall. They belongs to the family 16 of glycosyl hydrolases that includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.
395585	Glyco_hydro_16	1.47e-44	83	221	27	168	Glycosyl hydrolases family 16.
185683	Glyco_hydrolase_16	3.33e-26	87	239	65	208	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.
185684	GH16_lichenase	1.03e-18	83	221	62	193	lichenase, member of glycosyl hydrolase family 16. Lichenase, also known as 1,3-1,4-beta-glucanase, is a member of glycosyl hydrolase family 16, that specifically cleaves 1,4-beta-D-glucosidic bonds in mixed-linked beta glucans that also contain 1,3-beta-D-glucosidic linkages. Natural substrates of beta-glucanase are beta-glucans from grain endosperm cell walls or lichenan from the Islandic moss, Cetraria islandica. This protein is found not only in bacteria but also in anaerobic fungi. This domain includes two seven-stranded antiparallel beta-sheets that are adjacent to one another forming a compact, jellyroll beta-sandwich structure.
185693	GH16_laminarinase_like	8.40e-15	87	243	77	231	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QEO24396.1|GH16_18	4.13e-253	1	345	2	346
QWW25521.1|GH16_18	1.18e-252	1	345	2	346
QRG40470.1|GH16_18	2.29e-250	1	345	2	346
QEL62926.1|GH16	4.65e-161	108	345	1	224
QWU87017.1|GH16_18	1.80e-128	3	291	2	279

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6IBU_A	1.08e-47	20	251	2	232	Apo Crh5 transglycosylase [Aspergillus fumigatus Af293],6IBU_B Apo Crh5 transglycosylase [Aspergillus fumigatus Af293],6IBW_A Crh5 transglycosylase in complex with NAG [Aspergillus fumigatus Af293],6IBW_B Crh5 transglycosylase in complex with NAG [Aspergillus fumigatus Af293]
3H0O_A	2.04e-14	80	247	8	172	Chain A, Beta-glucanase [Fibrobacter succinogenes]
3WVJ_A	7.05e-14	85	221	70	196	Chain A, Beta-glucanase [Acetivibrio thermocellus ATCC 27405],3WVJ_B Chain B, Beta-glucanase [Acetivibrio thermocellus ATCC 27405]
3HR9_A	7.13e-14	80	247	9	173	Chain A, Beta-glucanase [Fibrobacter succinogenes]
3AXD_A	7.98e-14	80	247	11	175	The truncated Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase V18Y/W203Y in apo-form [Fibrobacter succinogenes subsp. succinogenes S85],3AXD_B The truncated Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase V18Y/W203Y in apo-form [Fibrobacter succinogenes subsp. succinogenes S85],3AXE_A The truncated Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase V18Y/W203Y in complex with cellotetraose (cellobiose density was observed) [Fibrobacter succinogenes subsp. succinogenes S85]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q5AK54|CRH12_CANAL	8.37e-68	22	256	43	274	Extracellular glycosidase CRH12 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=CRH12 PE=1 SV=1
sp|Q5AFA2|CRH11_CANAL	3.17e-65	14	267	16	270	Extracellular glycosidase CRH11 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=CRH11 PE=1 SV=1
sp|P53301|CRH1_YEAST	4.07e-60	22	286	40	302	Probable glycosidase CRH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=CRH1 PE=1 SV=1
sp|D4ALQ5|CRH11_ARTBC	6.93e-53	9	264	9	263	Probable extracellular glycosidase ARB_05253 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_05253 PE=1 SV=2
sp|Q8J0P4|CRF1_ASPFU	2.38e-45	20	251	23	253	Probable glycosidase crf1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=crf1 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000345	0.999604	CS pos: 19-20. Pr: 0.9584

TMHMM  Annotations     

There is no transmembrane helices in PSK78031.1.