CAZyme Information: PPTG_12094-t26_2-p1

Basic Information

• Species: Phytophthora parasitica
• Lineage: Oomycota; NA; ; Peronosporaceae; Phytophthora; Phytophthora parasitica
• CAZyme ID: PPTG_12094-t26_2-p1
• CAZy Family: GH5
• CAZyme Description: hypothetical protein, variant

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
937		106241.80	5.2462

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PparasiticaINRA-310	23240	761204	119	23121

• Gene Location: location=KI669589:597174-600268(+)

Full Sequence      

MRGLQLTFTALLATTTSLNAAIVPLDRWTFRSANGSVRVDNASVPGTSHVHLMEAGVIGD
PYYRFNEREYQWVVQETWTYETQVTLPSDVSSKPTLVFETLDGVAHVTVNGKQVATTANS
FVSHRIDVAAVLLPGVNHIQVEFQPVLQYTRNQADTYPYFVPATENFNTWTEPTRRSFAR
KAGSDFGWDWGPAFVTSGIAGAAYIEFGEPVVKLKDLQVVQVFPGGKEDLSVVDVTVRVA
LDGGKAKHENVTFDLFVNEEKKTTILTTIPEDWKEDTAIELLFRLENPKLWWSVGYGEPY
LYGIRVDAWNSDFNSTLSHKIGIRQVELVQDDTHAGNVAGKTFYFKINQVPIFIKGANWI
PADSFPTRTKASTISHLLESVRAANMNMVRVWGGGRYESDLFYAECDRLGILVWQELMFA
CGMYPRDTAFLQNVLKEVSVQVSRLRKYTSIAIWGGNNENENMMEQFVEAPIFPPGTTFN
RDIAVVDFTTLFVDLIRPIIVALDSSRPFVDTSPSNGLYSVDPYVKRWGPTNGVAFGDVH
FYDYNNDCQDYRMYPRARFISEFGFQSWPSADSLHNISSKDDWDSFQAFWKFLKYRERHE
NGTTQMVTQLERRFHVPFPFRNEKWLGEVSDVQDDKSFGFSISKRIESYLYLTQIQQSLC
YQTAIQTWRRGKNVELGMTMGILYWQLNDIWQGSSWSSIEYSGRWKSLHYVAKREFASFI
ISIHKREDSKAVEVYAVSDVNEDLHVDVVYEIRRTKCGSLVKSIDRQAITVAALESRLIE
ELDIEALLDHDATSCHHRSCFLHVHCVVKSSTTGLETAACEDTNHFFAPYKHLNLGWGEV
AAHSVVKTESGNAVVFSVELVATDFVALFVEVDVPGVLGYWSSNSFLMVGNETKMLNFTV
SASENVDMITVDAFSRLITVNWLQKSYDNSVEDVAVQ

Enzyme Prediction     

No EC number prediction in PPTG_12094-t26_2-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	27	814	7.9e-85	0.7194148936170213

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
225789	LacZ	3.69e-72	28	753	19	666	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
407658	Mannosidase_ig	1.49e-08	732	832	3	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
407625	Ig_mannosidase	8.61e-08	845	920	4	73	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
395572	Glyco_hydro_2	3.77e-05	212	324	1	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
236657	PRK10150	0.005	234	416	186	355	beta-D-glucuronidase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CCA25641.1|GH2	1.72e-254	19	925	56	989
AIG55460.1|GH2	3.53e-229	25	936	28	871
BAE24954.1|GH2|3.2.1.25	9.40e-149	28	896	29	854
BAE38715.1|GH2|3.2.1.25	2.60e-148	28	896	29	854
BDA42312.1|GH2	2.87e-148	28	899	39	898

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6DDT_A	1.85e-149	28	896	18	843	mouse beta-mannosidase (MANBA) [Mus musculus],6DDU_A mouse beta-mannosidase bound to beta-D-mannose (MANBA) [Mus musculus]
5N6U_A	3.24e-99	22	894	26	805	Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_B Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_C Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_D Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12]
2VJX_A	2.06e-98	28	899	15	817	Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	2.15e-98	28	899	17	819	Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	2.19e-98	28	899	17	819	Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q8K2I4|MANBA_MOUSE	1.28e-148	28	896	29	854	Beta-mannosidase OS=Mus musculus OX=10090 GN=Manba PE=1 SV=1
sp|Q95327|MANBA_CAPHI	9.18e-144	28	888	29	846	Beta-mannosidase OS=Capra hircus OX=9925 GN=MANBA PE=1 SV=1
sp|O00462|MANBA_HUMAN	3.56e-143	28	896	29	854	Beta-mannosidase OS=Homo sapiens OX=9606 GN=MANBA PE=1 SV=3
sp|Q29444|MANBA_BOVIN	1.38e-142	28	888	29	846	Beta-mannosidase OS=Bos taurus OX=9913 GN=MANBA PE=1 SV=1
sp|Q4FZV0|MANBA_RAT	3.24e-140	28	896	29	856	Beta-mannosidase OS=Rattus norvegicus OX=10116 GN=Manba PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000333	0.999620	CS pos: 20-21. Pr: 0.9781

TMHMM  Annotations     

There is no transmembrane helices in PPTG_12094-t26_2-p1.