CAZyme Information: PPTG_09868-t26_1-p1

Basic Information

• Species: Phytophthora parasitica
• Lineage: Oomycota; NA; ; Peronosporaceae; Phytophthora; Phytophthora parasitica
• CAZyme ID: PPTG_09868-t26_1-p1
• CAZy Family: GH3
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
824		92412.25	5.7109

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PparasiticaINRA-310	23240	761204	119	23121

• Gene Location: location=KI669581:599630-602250(+)

Full Sequence      

MAVNESSPLLQGHSAQQQQSGRPPLRRALYVIVPLALVAIATICILISSDGSKKSSAASS
SAPAPTKAPAEVKFTDAELLTMRPPFEEKLHPLPPKLVRRNDFGRSLPTNEFWSNLMVAD
SHGLNAGAGEVTLSPYTVRSLPKRLEISYGDSRRVATKDTITEYFNVDASFTAYSHKVLD
GNETGSALLGDGNSTSRNVVGFDVLSVLLQYYYGEENKWMKVPMVRGSPYVTVEYQNVLP
VLEFNATVSTVNGEKVEQKDPLTPWNELQTWTSNRFELDIEVYGAGESAHGTGKSQQKWI
LYFDKVRTLQLQYANEKDYRPYNWRGELTTPTNIRLVDTDFFTGAARLAIVPQGSSHAAI
KALDKAAAIYPVGSSITTDVKDTDASFSFCWKTKTFPDLGEPNLKSTSTAELLMMANPHH
VATFKTDSDAFQVLGQFGHRTLKSVMTAVLGSCWEMQDSLPTASFQEENVQIQPQYLEAI
KVALANDSNYTPEADDPYYFGKEIGRQARLVLIADKLDEKELRDDMLDKLEEWLTPWMLG
QNSDHFVYDRSWGGLCSLNGLKGVFWMTDFGNGWYNDHHFHYGYFLYAVAVVGKYRPAFV
KTHKAVVMSIVRDIASPDQSDTFFPFTRHFSWFDGHSFASGVYTLDGGKSQESVSEAINA
YYGVYLVGKSFKVPEVEHMGHLLLALEIRGAQTYWQMPSSSNIYEPIYAANKMTGQVAAT
KVSYTTWFGPQVEHMHLINMIPFTPITDAFLKPAYVKEEYPILQQQAFDRVQDPIEDRWK
GYAYLDLAIIDPADAWSKVQNMTFFDDGSSRTNSLYWIATRPTN

Enzyme Prediction     

No EC number prediction in PPTG_09868-t26_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH81	91	817	4.4e-156	0.9935691318327974

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
407548	Glyco_hydro81C	2.20e-98	477	815	7	349	Glycosyl hydrolase family 81 C-terminal domain. Family of eukaryotic beta-1,3-glucanases. Within the Aspergillus fumigatus protein, two perfectly conserved Glu residues (E550 or E554) have been proposed as putative nucleophiles of the active site of the Engl1 endoglucanase, while the proton donor would be D475. The endo-beta-1,3-glucanase activity is essential for efficient spore release. This entry represents the helical C-terminal domain.
227785	Acf2	1.99e-91	67	815	49	749	Endoglucanase Acf2 [Carbohydrate transport and metabolism].
397619	Glyco_hydro_81	4.93e-29	91	462	1	312	Glycosyl hydrolase family 81. Family of eukaryotic beta-1,3-glucanases. Within the Aspergillus fumigatus protein ENGL1, two perfectly conserved Glu residues (E550 or E554) have been proposed as putative nucleophiles of the active site of the Engl1 endoglucanase, while the proton donor would be D475. The endo-beta-1,3-glucanase activity is essential for efficient spore release.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UIZ24045.1|GH81	0.0	1	621	1	611
CCA19047.1|GH81	3.94e-271	5	821	9	815
UIZ24044.1|GH81	1.31e-132	622	824	5	207
AGP40408.1|GH81	9.09e-116	76	822	50	702
AUX36386.1|GH81	3.57e-114	76	822	61	713

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4K3A_A	5.14e-65	86	822	21	719	The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei],4K3A_B The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei]
5XBZ_A	7.36e-65	86	822	46	744	Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminaripentaose [Rhizomucor miehei],5XBZ_B Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminaripentaose [Rhizomucor miehei],5XC2_A Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei],5XC2_B Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei],5XC2_C Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei],5XC2_D Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei]
4K35_A	1.19e-63	86	822	21	719	The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei],4K35_B The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei]
6FOP_A	9.98e-38	492	818	334	653	Chain A, Glycoside hydrolase family 81 [Acetivibrio thermocellus ATCC 27405]
5V1W_A	2.03e-24	492	818	355	689	Chain A, Glycoside Hydrolase [Halalkalibacterium halodurans C-125]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q09850|ENG2_SCHPO	2.03e-54	82	812	12	693	Ascus wall endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng2 PE=1 SV=1
sp|Q9UT45|ENG1_SCHPO	6.62e-53	91	819	66	735	Primary septum endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng1 PE=3 SV=1
sp|D4AZ24|ENG1_ARTBC	1.81e-51	214	819	332	900	Probable endo-1,3(4)-beta-glucanase ARB_01444 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_01444 PE=1 SV=1
sp|Q5AIR7|ENG1_CANAL	2.30e-47	207	819	557	1139	Endo-1,3(4)-beta-glucanase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=ENG1 PE=1 SV=1
sp|Q12168|ENG2_YEAST	3.11e-47	217	815	212	772	Endo-1,3(4)-beta-glucanase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ACF2 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.999996	0.000020

TMHMM  Annotations     

Start	End
27	49