CAZyme Information: PPTG_05053-t26_1-p1

Basic Information

• Species: Phytophthora parasitica
• Lineage: Oomycota; NA; ; Peronosporaceae; Phytophthora; Phytophthora parasitica
• CAZyme ID: PPTG_05053-t26_1-p1
• CAZy Family: CE8
• CAZyme Description: trehalose-phosphatase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1214		136024.43	4.5340

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PparasiticaINRA-310	23240	761204	119	23121

• Gene Location: location=KI669567:219541-223185(-)

Full Sequence      

MAEGAAFKEREGALLRELSALYTKLNQVREKRVKAEQESGDNDHDTDDEGVKGSRRRRRV
LLVTRTLPFWLVSEDAQSQWRAEFPEHASLNGAMEIFQSLHENYDCVWIGSVHGEIEPSE
QNTLKEQLLQDRKYYPVFLDEKRERLYYQGFCKTVMWPLFHSCPPTTDDQITQHETMEGG
QEDERSMEKMWQAYVATNQAFADAVQEVYEEGDLVWIQGYHLTLVPQMVQNLFPNDNICL
GFFMHIPFPSAELYRILNNREEILTGMLGADLIGFQTYEYARHFQSACVRLLGLESSHKG
VDFNGHFARVTICPVGIDAEKYIAMVQSDAVQEQMKQLEGQFSDKKVILGVDQLDQTKGL
VHKLLAVEELFTHKPELAKEVVFLQIVTGASVYESALESQVESLVSRINSKLQDIGTEGP
VQYLNKEIAVEALIALYALADALVITPVRDGMNTVPFGYIVCREATDKHARVILSEFAGC
AQSLGGARLVNPWNTEELTDALQESLQDNDEVMRAHQHMYSYVSSFTSWHWADNFLEQLH
ECSEENALSVSGRELSRRDMTSAYSRSSRRLIFINYEGVLAVEASIPELAYPPQELIWQL
SMLTKDPRNTVVLVSARSTSVCEQWFAGLSGNLVLAAEYGVYVKWVGENEHWHCMVPNMD
MSWWEHVVPLLEYYTERTPGAYIERKDSSVAWHYRDCDLDHGLWQASELLVSLREITRSL
PVSVCPGNRYLEVRPQKVSKATLFERIWEFMNWSLLFPDEDPATTEYFGEPVHPPSSPMI
KPRGFHTDPIGEDDTTGFSLDDLEYRADRKDSVSFNMDAGALTLGQLEDVDDKHPVDFVL
VIASGDDRTDEDLFSFLVPPPIDLEAYCRQLEKDEFENLSDSEQEGIKPDIDESSSFSQR
SFILDAGESEGGIGSSSFGDISDDMSYNAFRRLLGRDGSSASLATGSGERGGLSAAASLA
RLSQPNDSDFLMLGENMTKLTPLAKPSISEGAANMFPVAMLNRPSNARRDEITEKSKFTP
RRDFPPSAALFKAMKVKYGDNFGIHRLPDTPAACWALVCPPTARAKLEDGVGQSSEGKSS
ITGSPDVERSPPGESSGNSSASSLSPPLPAEGSGNDEEHTRHETEDKSPKIIAAKELPEG
ESVSQTTSVSTDEAEDGRYRFPVNTFVCTLGRKLSQAPYFIKNSGDLFQLLQEMGMSSHI
RKQRMLSMGSVGDI

Enzyme Prediction     

EC	2.4.1.15:146

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT20	55	540	1.5e-148	0.9768421052631578

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
184712	PRK14501	0.0	99	781	36	698	putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional
340820	GT20_TPS	7.62e-169	99	540	36	463	trehalose-6-phosphate synthase. Trehalose-6-Phosphate Synthase (TPS, EC 2.4.1.15) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain.
395781	Glyco_transf_20	4.77e-155	107	541	47	470	Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex,.
215556	PLN03064	4.56e-152	103	747	133	776	alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional
177855	PLN02205	2.60e-147	102	783	103	812	alpha,alpha-trehalose-phosphate synthase [UDP-forming]

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UIZ28038.1|GT20	0.0	1	1214	1	1245
CCA15235.1|GT20	0.0	8	1207	28	1218
AJA04606.1|GT20	3.64e-155	50	747	120	806
AGT80036.1|GT20	3.64e-155	50	747	120	806
QOX06485.1|GT20	1.50e-154	8	757	16	760

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5HVM_A	3.79e-96	54	554	8	489	Structure of Aspergillus fumigatus trehalose-6-phosphate synthase A in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293],5HVM_B Structure of Aspergillus fumigatus trehalose-6-phosphate synthase A in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293]
5HUV_A	3.29e-95	59	548	5	476	Structure of Candida albicans trehalose-6-phosphate synthase E341R/E346R in complex with UDP-glucose [Candida albicans SC5314],5HUV_B Structure of Candida albicans trehalose-6-phosphate synthase E341R/E346R in complex with UDP-glucose [Candida albicans SC5314]
5HUT_A	4.55e-95	59	548	5	476	Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP-glucose [Candida albicans SC5314],5HUT_B Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP-glucose [Candida albicans SC5314],5HUU_A Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and glucose-6-phosphate [Candida albicans SC5314],5HUU_B Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and glucose-6-phosphate [Candida albicans SC5314],5HVL_A Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and validoxylamine A [Candida albicans SC5314],5HVL_B Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and validoxylamine A [Candida albicans SC5314]
6JBI_A	6.99e-91	59	540	1	463	Structure of Tps1 apo structure [Pyricularia oryzae 70-15],6JBI_B Structure of Tps1 apo structure [Pyricularia oryzae 70-15],6JBR_A Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_B Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_D Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_F Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_H Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_K Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_M Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_O Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBW_A Structure of Tps1/UDP complex [Pyricularia oryzae 70-15],6JBW_B Structure of Tps1/UDP complex [Pyricularia oryzae 70-15]
5HVO_A	1.38e-89	51	543	4	478	Structure of Aspergillus fumigatus trehalose-6-phosphate synthase B in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293],5HVO_B Structure of Aspergillus fumigatus trehalose-6-phosphate synthase B in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293],5HVO_C Structure of Aspergillus fumigatus trehalose-6-phosphate synthase B in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293],5HVO_D Structure of Aspergillus fumigatus trehalose-6-phosphate synthase B in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q54K57|TPSA_DICDI	6.19e-148	59	747	17	673	Alpha,alpha-trehalose-phosphate synthase [UDP-forming] A OS=Dictyostelium discoideum OX=44689 GN=tpsA PE=2 SV=1
sp|Q54NU9|TPSB_DICDI	3.97e-133	73	766	62	744	Alpha,alpha-trehalose-phosphate synthase [UDP-forming] B OS=Dictyostelium discoideum OX=44689 GN=tpsB PE=3 SV=1
sp|Q9LRA7|TPS9_ARATH	6.22e-129	35	747	38	765	Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] 9 OS=Arabidopsis thaliana OX=3702 GN=TPS9 PE=2 SV=1
sp|Q0WUI9|TPS8_ARATH	2.46e-128	41	747	39	760	Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] 8 OS=Arabidopsis thaliana OX=3702 GN=TPS8 PE=2 SV=1
sp|O80738|TPS10_ARATH	5.48e-127	57	759	58	777	Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] 10 OS=Arabidopsis thaliana OX=3702 GN=TPS10 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.999703	0.000300

TMHMM  Annotations     

There is no transmembrane helices in PPTG_05053-t26_1-p1.