CAZyme Information: POW18852.1

Basic Information

• Species: Puccinia striiformis
• Lineage: Basidiomycota; Pucciniomycetes; ; Pucciniaceae; Puccinia; Puccinia striiformis
• CAZyme ID: POW18852.1
• CAZy Family: GH5
• CAZyme Description: Beta-mannosidase [Source:UniProtKB/TrEMBL;Acc:A0A2S4WAR2]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1015		114586.66	8.5814

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Pstriiformis93TX-2	14629	N/A	0	14629

• Gene Location: location=PKSM01000058:235337-239871(-)

Full Sequence      

MPSCGLWLVSSLLSIHFYSLSVSAKIPVRGPPLPSTTLHFSTLKWSLANANRSIQVSTPF
LNQPHLALVKAGLIDEPNIGLNEGTTRWVGEEKYWTWETKFELSHAEEWQDVNNFYLIFN
GLDTYCDVQLGGEQVGSTDNAFRSWVFDGKAVNFRLTLTDSNMFSCAVTGIIRKNRNKSI
PMTLRFDSAWATATRLANQAGHTWFPPGSSGSNGRRATTVLRYEYSSRNWARKQQSDFGW
DWGPAYLPSGPTQPAYLIGLSGPGPPSIKEMNLHRGIQKSAPLRSSNGNADHHNGVVLQV
QKRRNNRENLVRHDADSPPLMWVHRSLIDIYRQGQRNNLPPNQSAPWIVNITLPVTSPRP
LSGYKPTLSASFPGTSIRLPTVQLTMLQPAKYRSFGPNSLWTAEFVIQPDAVELWYPSAL
GNPKLYDLKLTLEFASQHERSDTKNAPITQLTWYERVGFRTVIVDQSQYSDHEVSRGIRP
GTRFTFEINGKPFYAQGSSMIPIDTFAARTNSTTIRWLVESALLAHQNVIRTCDELGILV
WSEAVFACAAYPISPESFLDNIRAEVSENVARLSRHPSTALWAGNNEASCGEGYLMDVKH
TWPNGSVYFDQYDYLNNHVIRDLVLQNTRSASYIPSSTTQGYLSLEPYVSRYGASTSGEL
YGDQEHYNYDTSISFNTSSYPVARFVNEFGFHSLASIYTLDRILQSEEDYHFNSSVIRAH
NKNPPAGSLEYPYPADRGQNSLMTGVTRYYPTPNITGDARALLAQWAYASQVFQAAFMAS
QISYYRLGASRGERNMVNDVWEGTSWSSIEYTGRWKIFHYLAERVQSHVIITPIYHVKNE
TLDIYVTSDLWEDARGTAQLSWYDFAGHQLSKASKEFIVGPLNSTRLLRTSGLSAIVPNG
HPPHDAWLHLTLTTNDGKHANEQFFHPVKLKDCLLRPTKVSSRAIGHNQISLQVAHGVRG
YFKDMKTNQASNAIFLRPNETRNLEFVVKSYDHPDLTRNLESKMVVRTMWDNQHR

Enzyme Prediction     

EC	3.2.1.25:4

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	90	886	2.1e-45	0.6329787234042553

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
225789	LacZ	1.08e-28	403	825	241	628	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
407658	Mannosidase_ig	1.86e-15	840	932	1	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
407625	Ig_mannosidase	4.34e-04	955	1011	32	78	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
236657	PRK10150	0.004	403	497	233	296	beta-D-glucuronidase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CBQ72430.1|GH2	5.37e-161	41	871	87	864
SJX62291.1|GH2	7.02e-158	41	871	87	867
BCS20939.1|GH2	4.07e-155	37	1015	22	924
BCS16350.1|GH2	9.66e-155	41	1013	28	929
BCS04781.1|GH2	9.66e-155	41	1013	28	929

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4CVU_A	2.64e-134	44	1011	37	938	Structure of Fungal beta-mannosidase from Glycoside Hydrolase Family 2 of Trichoderma harzianum [Trichoderma harzianum],4UOJ_A Chain A, BETA-MANNOSIDASE GH2 [Trichoderma harzianum],4UOJ_B Chain B, BETA-MANNOSIDASE GH2 [Trichoderma harzianum]
6DDT_A	2.55e-61	45	853	18	702	mouse beta-mannosidase (MANBA) [Mus musculus],6DDU_A mouse beta-mannosidase bound to beta-D-mannose (MANBA) [Mus musculus]
2VJX_A	1.60e-48	65	941	35	765	Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2VQU_A	1.60e-48	65	941	35	765	Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQU_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron]
2JE8_A	1.62e-48	65	941	37	767	Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q0CI48|MANBA_ASPTN	1.31e-154	20	1011	12	927	Beta-mannosidase A OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndA PE=3 SV=2
sp|A2QWU9|MANBA_ASPNC	6.92e-154	16	1015	3	930	Beta-mannosidase A OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=mndA PE=3 SV=1
sp|Q5BCI8|MANBA_EMENI	1.68e-148	15	1013	1	925	Beta-mannosidase A OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mndA PE=3 SV=1
sp|A1DMT2|MANBA_NEOFI	3.78e-147	111	1015	90	929	Beta-mannosidase A OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=mndA PE=3 SV=1
sp|B8NP78|MANBA_ASPFN	5.81e-144	22	989	11	890	Beta-mannosidase A OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=mndA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.055834	0.944128	CS pos: 24-25. Pr: 0.6804

TMHMM  Annotations     

There is no transmembrane helices in POW18852.1.