CAZyme Information: POW10677.1

Basic Information

• Species: Puccinia striiformis
• Lineage: Basidiomycota; Pucciniomycetes; ; Pucciniaceae; Puccinia; Puccinia striiformis
• CAZyme ID: POW10677.1
• CAZy Family: GH5
• CAZyme Description: unspecified product

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
722		80596.20	7.9913

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Pstriiformis93-210	15090	N/A	0	15090

• Gene Location: location=PKSL01000044:158747-161507(-)

Full Sequence      

MSCLQGSQVDGDLFAPVDSQRKHAAPPLPLFGQGCAHPQLPLHISEDCRHRPIGTNKWYT
QLMLTPNGTDPIYPLPYAMAFLDGDSVLSRPGGKDSKPLIGLGILHSTASQRVFGPPVGQ
EADSSVRYYYNPVTVSVCFGAKEFSPSNIVPSMSNWSELAVQFELSLPTNLSSKSRISFP
ISRGSAYVTALYTALTPTLGSTHSIISLECANVGEEHHLYGFRKHRIGYSDGSIWLVYSL
AQQAGCTSHLNLQKITPTDLGHPGAWSGIIQLVKMSDHYGRQLEENSHRESEEFVYDHGI
GVWTHAATLRSGPMGSGQYSFDWITGGPRCNSVAPLMFALPHHTEALSDPTIVVKPEIRM
QSRVTGVMRLCRAARWLFKENVADLRRYGIKPDYLPNGPPLYDALKIDLLRQVMVKEIDT
DFDAESDLDSYYFSGKKLAKQALQCLTASSILQDRDLAGKFVEKTKKSFDKFCRNRSKAA
PLVYENTWKGIISSSILRTGNRNDDFGNGVYNDHHFHYAYFIHTAAVLVYHDHSYLHSIR
DYVHDLIRDVNNSDQSDPYFPLFRNFDWFLGHSLATGLDTNIDGKNQESCSEDANVKVWA
EVTQNPRLGALADLQLSLFKRSVNHYYFMQEANTKAPACFKGNRVPGILFENKADYTTFF
SNEKDAIHMIQVIPITPITSSSAVLSSFGKNGPTRARLQKTRGRLSNVTLDSIRDRGPKL
RV

Enzyme Prediction     

EC	3.2.1.39:20

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH81	37	680	2.8e-137	0.8810289389067524

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
227785	Acf2	2.64e-137	36	680	74	682	Endoglucanase Acf2 [Carbohydrate transport and metabolism].
407548	Glyco_hydro81C	9.86e-122	407	680	7	282	Glycosyl hydrolase family 81 C-terminal domain. Family of eukaryotic beta-1,3-glucanases. Within the Aspergillus fumigatus protein, two perfectly conserved Glu residues (E550 or E554) have been proposed as putative nucleophiles of the active site of the Engl1 endoglucanase, while the proton donor would be D475. The endo-beta-1,3-glucanase activity is essential for efficient spore release. This entry represents the helical C-terminal domain.
397619	Glyco_hydro_81	1.75e-65	37	381	1	308	Glycosyl hydrolase family 81. Family of eukaryotic beta-1,3-glucanases. Within the Aspergillus fumigatus protein ENGL1, two perfectly conserved Glu residues (E550 or E554) have been proposed as putative nucleophiles of the active site of the Engl1 endoglucanase, while the proton donor would be D475. The endo-beta-1,3-glucanase activity is essential for efficient spore release.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ABH10633.1|GH81	4.55e-103	37	680	25	637
CBF89420.1|GH81|3.2.1.39	7.48e-103	52	681	223	828
EAA66571.1|GH81	1.28e-102	52	681	215	820
ATY59065.1|GH81	1.38e-101	11	680	296	932
QGA20515.1|GH81	2.00e-99	52	677	198	801

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4K3A_A	4.22e-75	7	677	1	646	The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei],4K3A_B The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei]
5XBZ_A	6.46e-75	7	677	26	671	Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminaripentaose [Rhizomucor miehei],5XBZ_B Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminaripentaose [Rhizomucor miehei],5XC2_A Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei],5XC2_B Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei],5XC2_C Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei],5XC2_D Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei]
4K35_A	1.01e-71	7	677	1	646	The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei],4K35_B The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei]
6FOP_A	5.11e-19	386	676	301	582	Chain A, Glycoside hydrolase family 81 [Acetivibrio thermocellus ATCC 27405]
5V1W_A	4.09e-13	392	674	326	604	Chain A, Glycoside Hydrolase [Halalkalibacterium halodurans C-125]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q09850|ENG2_SCHPO	8.58e-96	36	679	20	628	Ascus wall endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng2 PE=1 SV=1
sp|D4AZ24|ENG1_ARTBC	1.17e-92	24	677	182	822	Probable endo-1,3(4)-beta-glucanase ARB_01444 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_01444 PE=1 SV=1
sp|Q12168|ENG2_YEAST	1.05e-84	4	680	65	705	Endo-1,3(4)-beta-glucanase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ACF2 PE=1 SV=1
sp|Q5AIR7|ENG1_CANAL	2.14e-79	7	680	422	1068	Endo-1,3(4)-beta-glucanase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=ENG1 PE=1 SV=1
sp|Q9UT45|ENG1_SCHPO	2.44e-77	27	680	57	664	Primary septum endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng1 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.999966	0.000075

TMHMM  Annotations     

There is no transmembrane helices in POW10677.1.