CAZyme Information: POW10109.1

Basic Information

• Species: Puccinia striiformis
• Lineage: Basidiomycota; Pucciniomycetes; ; Pucciniaceae; Puccinia; Puccinia striiformis
• CAZyme ID: POW10109.1
• CAZy Family: GH5
• CAZyme Description: unspecified product

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1024		114714.96	6.7084

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Pstriiformis93-210	15090	N/A	0	15090

• Gene Location: location=PKSL01000049:182828-187329(+)

Full Sequence      

MYLFSSLFCLFSLRSLPLAVLAAASSRGPPLPSTQISLSSLKWTLANANRSIVVPTPFLN
QPHLALIKAGVIDDPNIGLNEGTTRWVGEEEAWTWETKVKIDTSGKWADVERTTRSEAGY
LMASLQCFDDGKSDKLCKICLLRIIFHSTIATQVIQGSLNQTVPLSLRFASAYATASRQA
HEPSNMWFPPKSADKKNSTTLQPFAYSYRNWARKQQSDFGWDWGPGYLPSGPIQPAYIIG
LSASAQSSLASGNQTNSNQIQTKVLPKQRSIVIGTAHKQLSLHAEKKHKRATEETNGAPL
LWVHRTIIDIYRKGQLNNLLPDQAAPWVINITLPITSPKLLNATLSGVLSGTSVKLPAKQ
LSVLKTPVYRDFGPDYSLTVEYIVDPKEVELWYPATLGSPTLYDLELTLQVDGGVTSPAN
TNNALVWREKVGFRTIVVDQSRYTDQEVASGIRPGTRFTFIINGKPFYVQGSSMIPIDNF
AARTNSTTIRWLLESALLANQNVIRIWGGGAYQTDEFYDMCDELGILAWSESVFACGAYP
LSPESFLDNIRAEVSENVARLNRHPSTALWAGNNEGEGYLIDVNRTWTNGSIYFNQYDHL
NNHVLRELVLDNTRSISYIPSSTTQGYLSLDPYVARYYNSTPGEIYGDKEHYNYNTSVSF
DVSSYPVGRFVNEFGFHSMPSIYTWDRVLKSPQDYDFNSTVIRAHNKHNPAESLAYPWPA
DDGQMQMTTGVTDHYPTPNITGDYHTQLAQWAYSTQVFQAAFMASEIQYYRLGASRGENN
MGAVYWQLNDVWEGISWSSIEYTGRWKVFHYIAARVQSHVIISPIFHQKNNTLDIYVTSD
LWQPVEGTAEWTWYDFAGQQLAAPTKKSFSVSPIHSTQLYNAGSLSEMVPNGHPPDDAWL
HLTMSTSDGKYTNEQFFHPVPLKDCQLRATKVHSRPIGHNQVSIAVASGGVAAWVNVEHP
PGVRGYFRDLTTLLPSNAFFLRPDEERHLEFVLKGQDSDHDGLTKDLASKMVVRTLWDNQ
HPSQ

Enzyme Prediction     

EC	3.2.1.25:4

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	324	906	1.2e-51	0.4787234042553192

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
225789	LacZ	1.16e-35	353	816	217	628	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
407658	Mannosidase_ig	1.86e-15	831	924	1	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
407625	Ig_mannosidase	4.16e-10	939	1018	12	78	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
236657	PRK10150	1.26e-05	389	579	242	421	beta-D-glucuronidase; Provisional
236673	ebgA	3.28e-05	337	575	232	449	cryptic beta-D-galactosidase subunit alpha; Reviewed

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCS20939.1|GH2	2.84e-180	36	1020	23	922
CBF85479.1|GH2	7.31e-178	19	1020	10	925
EAA64028.1|GH2	7.31e-178	19	1020	10	925
BCS16350.1|GH2	8.91e-177	38	1020	27	929
BCS04781.1|GH2	8.91e-177	38	1020	27	929

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4CVU_A	4.76e-172	12	1022	3	942	Structure of Fungal beta-mannosidase from Glycoside Hydrolase Family 2 of Trichoderma harzianum [Trichoderma harzianum],4UOJ_A Chain A, BETA-MANNOSIDASE GH2 [Trichoderma harzianum],4UOJ_B Chain B, BETA-MANNOSIDASE GH2 [Trichoderma harzianum]
6DDT_A	1.71e-78	43	841	18	699	mouse beta-mannosidase (MANBA) [Mus musculus],6DDU_A mouse beta-mannosidase bound to beta-D-mannose (MANBA) [Mus musculus]
2VJX_A	5.00e-58	33	976	5	809	Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	5.11e-58	33	976	7	811	Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	5.16e-58	33	976	7	811	Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q0CI48|MANBA_ASPTN	1.70e-184	18	1018	9	927	Beta-mannosidase A OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndA PE=3 SV=2
sp|Q5BCI8|MANBA_EMENI	1.30e-178	19	1020	10	925	Beta-mannosidase A OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mndA PE=3 SV=1
sp|A2QWU9|MANBA_ASPNC	3.91e-174	38	1020	27	928	Beta-mannosidase A OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=mndA PE=3 SV=1
sp|B8NP78|MANBA_ASPFN	6.72e-174	19	1018	7	909	Beta-mannosidase A OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=mndA PE=3 SV=1
sp|Q2UN00|MANBA_ASPOR	1.33e-173	19	1018	7	909	Beta-mannosidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=mndA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.575330	0.424669

TMHMM  Annotations     

There is no transmembrane helices in POW10109.1.