CAZyme Information: POW08728.1

Basic Information

• Species: Puccinia striiformis
• Lineage: Basidiomycota; Pucciniomycetes; ; Pucciniaceae; Puccinia; Puccinia striiformis
• CAZyme ID: POW08728.1
• CAZy Family: GH18
• CAZyme Description: unspecified product

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
722		82477.58	6.8978

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Pstriiformis93TX-2	14629	N/A	0	14629

• Gene Location: location=PKSM01000134:130624-133725(-)

Full Sequence      

MDDPEPRTNNKNRSSVSFDGRHFIDTETEQVISFRGVSLSGCSKLPSKPDGRTHQAELFF
EHRQVSFVGHPLKLEHAPHYLSQLVSWGFNLIRLVICWEALEHSGPGIYDLEYIDYITQL
VQICEELNLNVLVDAHQDVWSRLCGGSGAPGWTLELAGFEIANLSETGAAALQQLGAPKG
VWPSGYQKLAAGTMFTLFFSGDKFAPNRKVKRNLHQKWAKEGTDGEEMISLQEFLQSSMV
EAFGQLADSLSRFDCVIGFEPMNEPHRGFINLHSPHQWNPMTDLFIRDCPSFLEAVALGE
GHSQRIDVYTPTWPVPSFRFHTRRVKPEVRAWKSSVECIWKEHGVWRWDEKRNKPVVLKP
KHFNLDPLTGKPFDFYSQALYPFVSRFAKRVQRYRSDWIIPVGPIPNEFYPKWDSCQRPK
NLIAGPHFYDLFSLVHKAHGTLTMDVQGICMNKPIWKWMHFGHIAAKKNYTEQIKNIVNS
VYNNLGEIPCLIGELGICMDLNRGEAFKTGNYYWQHYQMNALLSACESNLVSYVLWNFNP
YNTDEYGDGWNGENFSFISQSEVDSSSPYSQPRILSAIVRPSARKVSGIPCRSTYDSENS
TFEFEYKNPDLNPKGEQTSRFKTTETEIFLPAERFPVDQIHISVSDGNYSHGADTQLLIW
KHSNLEPGAKHWIKITSPIRVKIEKPVSENWPVTSWLSLTLLLSLAVALLALFWSSDQKI
SL

Enzyme Prediction     

EC	3.2.1.45:1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH5	28	543	1.5e-165	0.9925788497217068

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
408348	Glyco_hydro_5_C	1.74e-21	580	675	1	86	Glycoside hydrolase family 5 C-terminal domain. This is the C-terminal domain of endo-glycoceramidase II (EGC), a membrane-associated family 5 glycosidase pfam00150. The C-terminal domain assumes a beta-sandwich fold, which resembles that of many carbohydrate-binding modules.
395098	Cellulase	2.91e-06	80	138	29	88	Cellulase (glycosyl hydrolase family 5).
225344	BglC	3.90e-06	80	169	78	167	Aryl-phospho-beta-D-glucosidase BglC, GH1 family [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ULM60693.1|GH5_12	1.05e-184	13	675	69	813
UPL04364.1|GH5_12	2.12e-176	16	690	19	746
QKD54802.1|GH5_12	2.21e-176	22	663	24	718
QGI82100.1|GH5_12	6.97e-175	22	663	24	718
QGI64843.1|GH5_12	6.97e-175	22	663	24	718

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7LPO_A	1.60e-72	22	573	27	608	Chain A, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPO_B Chain B, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPO_C Chain C, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPO_D Chain D, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPP_A Chain A, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPP_B Chain B, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPP_C Chain C, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPP_D Chain D, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPQ_A Chain A, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPQ_B Chain B, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPQ_C Chain C, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPQ_D Chain D, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99]
5CCU_A	2.11e-06	77	155	81	158	Chain A, Putative secreted endoglycosylceramidase [Rhodococcus hoagii 103S],5CCU_B Chain B, Putative secreted endoglycosylceramidase [Rhodococcus hoagii 103S]
5J14_A	2.11e-06	77	155	81	158	Chain A, Putative secreted endoglycosylceramidase [Rhodococcus hoagii 103S],5J14_B Chain B, Putative secreted endoglycosylceramidase [Rhodococcus hoagii 103S],5J7Z_A Chain A, Putative secreted endoglycosylceramidase [Rhodococcus hoagii 103S],5J7Z_B Chain B, Putative secreted endoglycosylceramidase [Rhodococcus hoagii 103S]
2OSW_A	3.57e-06	88	161	79	163	Endo-glycoceramidase II from Rhodococcus sp. [Rhodococcus sp.],2OSW_B Endo-glycoceramidase II from Rhodococcus sp. [Rhodococcus sp.],2OYK_A Endo-glycoceramidase II from Rhodococcus sp.: cellobiose-like isofagomine complex [Rhodococcus sp.],2OYK_B Endo-glycoceramidase II from Rhodococcus sp.: cellobiose-like isofagomine complex [Rhodococcus sp.],2OYL_A Endo-glycoceramidase II from Rhodococcus sp.: cellobiose-like imidazole complex [Rhodococcus sp.],2OYL_B Endo-glycoceramidase II from Rhodococcus sp.: cellobiose-like imidazole complex [Rhodococcus sp.],2OYM_A Endo-glycoceramidase II from Rhodococcus sp.: five-membered iminocyclitol complex [Rhodococcus sp.],2OYM_B Endo-glycoceramidase II from Rhodococcus sp.: five-membered iminocyclitol complex [Rhodococcus sp.]
2OSX_A	3.57e-06	88	161	79	163	Chain A, Endoglycoceramidase II [Rhodococcus sp.]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|I1BTD7|EGCR1_RHIO9	3.70e-156	16	667	15	660	Glucosylceramidase OS=Rhizopus delemar (strain RA 99-880 / ATCC MYA-4621 / FGSC 9543 / NRRL 43880) OX=246409 GN=ERC1 PE=1 SV=1
sp|H1AE12|EGCR1_CRYNH	7.75e-156	14	720	14	732	Glucosylceramidase OS=Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) OX=235443 GN=EGC1 PE=1 SV=1
sp|H1AE13|EGCR1_ASPFM	7.08e-84	19	675	9	728	Glucosylceramidase OS=Neosartorya fumigata OX=746128 GN=egc1 PE=1 SV=1
sp|P40566|EGH1_YEAST	4.02e-79	16	690	7	753	Ergosteryl-beta-glucosidase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=EGH1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000041	0.000005

TMHMM  Annotations     

Start	End
692	714