Species | Puccinia striiformis | |||||||||||
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Lineage | Basidiomycota; Pucciniomycetes; ; Pucciniaceae; Puccinia; Puccinia striiformis | |||||||||||
CAZyme ID | POW07923.1 | |||||||||||
CAZy Family | GH18 | |||||||||||
CAZyme Description | Chitin deacetylase [Source:UniProtKB/TrEMBL;Acc:A0A2S4VEH6] | |||||||||||
CAZyme Property |
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Genome Property |
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Gene Location |
EC | 3.1.1.72:1 | 3.5.1.41:1 |
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Family | Start | End | Evalue | family coverage |
---|---|---|---|---|
CE4 | 40 | 165 | 5e-29 | 0.9153846153846154 |
CE4 | 998 | 1112 | 1.7e-20 | 0.9153846153846154 |
Cdd ID | Domain | E-Value | qStart | qEnd | sStart | sEnd | Domain Description |
---|---|---|---|---|---|---|---|
341237 | Firefly_Luc_like | 1.06e-99 | 364 | 947 | 37 | 486 | Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL). This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light. |
223395 | CaiC | 2.08e-77 | 364 | 829 | 65 | 472 | Acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II [Lipid transport and metabolism, Secondary metabolites biosynthesis, transport and catabolism]. |
341230 | 4CL | 1.62e-76 | 364 | 951 | 59 | 505 | 4-Coumarate-CoA Ligase (4CL). 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids. |
200575 | CE4_ClCDA_like | 4.13e-70 | 36 | 230 | 1 | 197 | Catalytic NodB homology domain of Colletotrichum lindemuthianum chitin deacetylase and similar proteins. This family is represented by the chitin deacetylase (endo-chitin de-N-acetylase, ClCDA, EC 3.5.1.41) from Colletotrichum lindemuthianum (also known as Glomerella lindemuthiana), which is a member of the carbohydrate esterase 4 (CE4) superfamily. ClCDA catalyzes the hydrolysis of N-acetamido groups of N-acetyl-D-glucosamine residues in chitin, converting it to chitosan in fungal cell walls. It consists of a single catalytic domain similar to the deformed (alpha/beta)8 barrel fold adopted by other CE4 esterases, which encompasses a mononuclear metalloenzyme employing a conserved His-His-Asp zinc-binding triad closely associated with the conserved catalytic base (aspartic acid) and acid (histidine), to carry out acid/base catalysis. It possesses a highly conserved substrate-binding groove, with subtle alterations that influence substrate specificity and subsite affinity. Unlike its bacterial homologs, ClCDA contains two intramolecular disulfide bonds that may add stability to this secreted protein. The family also includes many uncharacterized deacetylases and hypothetical proteins mainly from eukaryotes, which show high sequence similarity to ClCDA. |
341228 | AFD_class_I | 3.83e-66 | 508 | 843 | 1 | 310 | Adenylate forming domain, Class I, also known as the ANL superfamily. This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain. |
Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End |
---|---|---|---|---|---|
4.48e-97 | 37 | 254 | 38 | 255 | |
2.20e-67 | 35 | 254 | 54 | 269 | |
1.71e-57 | 8 | 254 | 13 | 251 | |
8.80e-57 | 967 | 1206 | 11 | 248 | |
4.14e-56 | 967 | 1206 | 11 | 248 |
Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
---|---|---|---|---|---|---|
1.91e-48 | 509 | 828 | 183 | 483 | Crystal structure of 4-coumarate:CoA ligase delta-V341 mutant complexed with feruloyl adenylate [Nicotiana tabacum],5BSW_B Crystal structure of 4-coumarate:CoA ligase delta-V341 mutant complexed with feruloyl adenylate [Nicotiana tabacum] |
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2.86e-47 | 509 | 828 | 183 | 484 | Crystal structure of 4-coumarate:CoA ligase complexed with magnesium and Adenosine triphosphate [Nicotiana tabacum],5BSM_B Crystal structure of 4-coumarate:CoA ligase complexed with magnesium and Adenosine triphosphate [Nicotiana tabacum],5BSR_A Crystal structure of 4-coumarate:CoA ligase complexed with adenosine monophosphate and Coenzyme A [Nicotiana tabacum],5BST_A Crystal structure of 4-coumarate:CoA ligase complexed with coumaroyl adenylate [Nicotiana tabacum],5BSU_A Crystal structure of 4-coumarate:CoA ligase complexed with caffeoyl adenylate [Nicotiana tabacum],5BSV_A Crystal structure of 4-coumarate:CoA ligase complexed with feruloyl adenylate [Nicotiana tabacum] |
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5.20e-47 | 509 | 828 | 183 | 484 | Structure of the open conformation of 4-coumarate-CoA ligase from Nicotiana tabacum [Nicotiana tabacum],5U95_B Structure of the open conformation of 4-coumarate-CoA ligase from Nicotiana tabacum [Nicotiana tabacum],5U95_C Structure of the open conformation of 4-coumarate-CoA ligase from Nicotiana tabacum [Nicotiana tabacum],5U95_D Structure of the open conformation of 4-coumarate-CoA ligase from Nicotiana tabacum [Nicotiana tabacum] |
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1.14e-46 | 509 | 855 | 180 | 508 | Crystal structures and enzymatic mechanisms of a Populus tomentosa 4-coumarate--CoA ligase [Populus tomentosa],3A9V_A Crystal structures and enzymatic mechanisms of a Populus tomentosa 4-coumarate--CoA ligase [Populus tomentosa],3NI2_A Crystal structures and enzymatic mechanisms of a Populus tomentosa 4-coumarate:CoA ligase [Populus tomentosa] |
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6.06e-44 | 364 | 832 | 115 | 532 | 4-Coumaroyl-CoA Ligase::Stilbene Synthase fusion protein [Arabidopsis thaliana] |
Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
---|---|---|---|---|---|---|
1.24e-49 | 492 | 828 | 159 | 485 | 4-coumarate-CoA ligase 1 OS=Narcissus pseudonarcissus OX=39639 GN=4CL1 PE=2 SV=1 |
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2.65e-49 | 364 | 840 | 90 | 510 | 4-coumarate--CoA ligase 2 OS=Arabidopsis thaliana OX=3702 GN=4CL2 PE=1 SV=2 |
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3.92e-49 | 364 | 834 | 102 | 515 | 4-coumarate--CoA ligase 3 OS=Arabidopsis thaliana OX=3702 GN=4CL3 PE=1 SV=1 |
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4.32e-48 | 509 | 828 | 186 | 487 | 4-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1 |
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7.71e-48 | 365 | 828 | 81 | 485 | 4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1 |
Other | SP_Sec_SPI | CS Position |
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0.001339 | 0.998618 | CS pos: 24-25. Pr: 0.9549 |
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