CAZyme Information: POW05143.1

Basic Information

• Species: Puccinia striiformis
• Lineage: Basidiomycota; Pucciniomycetes; ; Pucciniaceae; Puccinia; Puccinia striiformis
• CAZyme ID: POW05143.1
• CAZy Family: GH16|GH16
• CAZyme Description: unspecified product

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
554		61457.57	7.0116

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Pstriiformis93-210	15090	N/A	0	15090

• Gene Location: location=PKSL01000102:37312-39520(+)

Full Sequence      

MPGLATSQILLPPVLLIGSLKPTLPAWSTSHQNSTDSSYPTRFRGVRWDNEQWKLVNTVL
EPGRYQSRISLANGYLGINLAALARFLKGPSGKQLPQRLACLRSPPKFCYHRGFYDYQLK
LNSSNFPWLINTIRNFQSSLDLKAGLMRWKFDWILDGVQPMISIEYTMFVNKLHINRAAV
QLKLKAADDIELRVVDLLDGECAVRTDMVEKHHHSNSSTICSAVSPNGMAHLTGHVCSSL
RSKAFSRSELFEGLPQLTDHSTSTIAQSASLKLKREQSVVVEKFIGAASGDAFSNPQAIA
LQSALAGADDGFAAQLDSHRKEWSSIMTEDSVDDYGLPEQGPSRIDPDIVELQILAITNP
FHLLQNTLSSNAIALSDNSTALDNWSISVCGLGSSCYGGMIFWDAEVWMAPGLVLSHPQA
AQRIINYRVAQFPQAQENLKMAYSSSQNQTNQFSPGDAIYPWTSGRFGNCTASGPCFDYE
YHINGDIGLSFYHHLVATGNFNSFKRNLLPIYQAIAGLFSNLLSETSDWKYGLRNATDPD
EYANQLTIRPIRCF

Enzyme Prediction     

EC	3.2.1.28:5

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH65	359	546	5.7e-41	0.45161290322580644

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
224471	ATH1	4.61e-41	51	544	9	497	Trehalose and maltose hydrolase (possible phosphorylase) [Carbohydrate transport and metabolism].
397616	Glyco_hydro_65N	2.23e-25	63	289	11	239	Glycosyl hydrolase family 65, N-terminal domain. This family of glycosyl hydrolases contains vacuolar acid trehalase and maltose phosphorylase.Maltose phosphorylase (MP) is a dimeric enzyme that catalyzes the conversion of maltose and inorganic phosphate into beta-D-glucose-1-phosphate and glucose. This domain is believed to be essential for catalytic activity although its precise function remains unknown.
281612	Glyco_hydro_65m	4.52e-14	359	544	3	179	Glycosyl hydrolase family 65 central catalytic domain. This family of glycosyl hydrolases contains vacuolar acid trehalase and maltose phosphorylase.Maltose phosphorylase (MP) is a dimeric enzyme that catalyzes the conversion of maltose and inorganic phosphate into beta-D-glucose-1-phosphate and glucose. The central domain is the catalytic domain, which binds a phosphate ion that is proximal the the highly conserved Glu. The arrangement of the phosphate and the glutamate is thought to cause nucleophilic attack on the anomeric carbon atom. The catalytic domain also forms the majority of the dimerization interface.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QIW95062.1|GH65	4.74e-158	37	547	21	566
CBX91091.1|GH65	2.86e-150	2	546	4	579
CAE7215092.1|GH65	5.05e-147	28	546	16	571
AQA29363.1|GH65	5.37e-146	35	544	16	559
UJO19712.1|GH65	1.49e-145	35	544	16	559

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7FE3_A	4.13e-18	133	546	100	474	Chain A, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE3_B Chain B, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE3_C Chain C, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE4_A Chain A, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE4_B Chain B, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE4_C Chain C, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101]
3WIQ_A	5.84e-07	386	542	326	484	Crystal structure of kojibiose phosphorylase complexed with kojibiose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_A Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_B Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_C Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_D Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|D4B5H9|ATC1_ARTBC	5.76e-124	39	544	53	589	Cell wall acid trehalase ARB_03719 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_03719 PE=1 SV=1
sp|P78617|TREA_EMENI	8.96e-122	31	546	45	589	Acid trehalase OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=treA PE=3 SV=1
sp|M1RNJ8|ATH1_CANGB	8.87e-42	43	546	157	682	Periplasmic/secreted acid trehalase ATH1 OS=Candida glabrata OX=5478 GN=ATH1 PE=1 SV=1
sp|Q5AAU5|ATC1_CANAL	1.17e-40	130	546	233	612	Cell wall acid trehalase ATC1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=ATC1 PE=1 SV=2
sp|N1P212|ATH1_YEASC	2.09e-36	43	546	121	649	Periplasmic acid trehalase ATC1 OS=Saccharomyces cerevisiae (strain CEN.PK113-7D) OX=889517 GN=CENPK1137D_1727 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.980164	0.019876

TMHMM  Annotations     

There is no transmembrane helices in POW05143.1.