CAZyme Information: PMAA_096570-t26_1-p1

Basic Information

• Species: Talaromyces marneffei
• Lineage: Ascomycota; Eurotiomycetes; ; Trichocomaceae; Talaromyces; Talaromyces marneffei
• CAZyme ID: PMAA_096570-t26_1-p1
• CAZy Family: GT22
• CAZyme Description: alpha-galactosidase, putative

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
646	DS995902|CGC31	69712.49	4.5221

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_TmarneffeiATCC18224	10194	441960	145	10049

• Gene Location: location=DS995902:1719349-1721540(+)

Full Sequence      

MVGVGLTSSPPTHARTQNRLAQTPQMGWNTYNHYGCSPNEAIVHSNAQALVDLGLASLGY
RYVTTDCGWTVADRLPNGSLTWDSTRFPSGFPALGQYLHGLGLLFGVYQDAGILLCGSPP
NQIGSLYHELQDAQTFASWGVDSLKYDNCYSDAATNYPNVNYAPSTSPEPRYANMSNALS
QQDRAILFQVCEWGVDFPAAWAPSLGNSWRIGNDISSSWTSIYRLVNQIAPQTDFAGPGQ
WPDLDMLEVGNNVYSTAEEQTHFSLWAILKSPLVIGAALKDAATSINEESLAILKQKDVI
SYNQDELSVSANLSRRYTEDEYDVWSGPLSGKRIVAALVNWADEKRYLTIALPDIGIQYA
GMVKDIWKNVTIQDVKTSYTAEVEAHGTMLLELQNTTSLGEYSTNIFGTSTGNSTTFEAI
YGLTDSSNYTLSITFISTNSASEVMTVKSSASSQTSTVTVAPNTASVSLPITLNAGTTNS
LTIHHQLSIESISITPPEGTYYSNTKFTTTGYATSTACGIGYCAPVGSKIGYIRPGGTAV
TAISATSAGTKFVEIDYINNEISFWSGWNARNITVTINGGTPTRLEVPMSGRHSELFGPG
RGWWDTATLGVLTNGWVQGENEVVIGNDGGAGYSLYAADFVGLRVY

Enzyme Prediction     

EC	3.2.1.22:2

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH27	124	371	5.6e-68	0.9737991266375546

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
269893	GH27	5.60e-119	24	305	1	271	glycosyl hydrolase family 27 (GH27). GH27 enzymes occur in eukaryotes, prokaryotes, and archaea with a wide range of hydrolytic activities, including alpha-glucosidase (glucoamylase and sucrase-isomaltase), alpha-N-acetylgalactosaminidase, and 3-alpha-isomalto-dextranase. All GH27 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. GH27 members are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively.
166449	PLN02808	4.56e-108	18	386	26	376	alpha-galactosidase
177874	PLN02229	1.81e-104	18	388	57	412	alpha-galactosidase
178295	PLN02692	8.56e-96	18	394	50	409	alpha-galactosidase
374582	Melibiase_2	9.43e-65	23	305	1	284	Alpha galactosidase A.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QGA19200.1|CBM35|GH27	0.0	1	646	1	646
BCR99189.1|CBM35|GH27	3.51e-310	15	645	24	659
UDD59118.1|CBM35|GH27	1.70e-302	10	645	18	656
QMW29010.1|CBM35|GH27	3.54e-297	10	645	18	656
QMW41085.1|CBM35|GH27	3.54e-297	10	645	18	656

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6F4C_B	8.44e-90	18	386	3	353	Nicotiana benthamiana alpha-galactosidase [Nicotiana benthamiana]
1UAS_A	3.15e-85	17	367	2	334	Chain A, alpha-galactosidase [Oryza sativa]
3A5V_A	1.11e-76	18	395	3	392	Crystal structure of alpha-galactosidase I from Mortierella vinacea [Umbelopsis vinacea]
3LRK_A	3.89e-66	18	355	24	376	Chain A, Alpha-galactosidase 1 [Saccharomyces cerevisiae],3LRL_A Chain A, Alpha-galactosidase 1 [Saccharomyces cerevisiae]
3LRM_A	5.51e-65	18	355	24	376	Chain A, Alpha-galactosidase 1 [Saccharomyces cerevisiae],3LRM_B Chain B, Alpha-galactosidase 1 [Saccharomyces cerevisiae],3LRM_C Chain C, Alpha-galactosidase 1 [Saccharomyces cerevisiae],3LRM_D Chain D, Alpha-galactosidase 1 [Saccharomyces cerevisiae]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|A2R2S6|AGALD_ASPNC	1.53e-306	6	645	14	658	Probable alpha-galactosidase D OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=aglD PE=3 SV=2
sp|Q2UI87|AGALD_ASPOR	1.00e-301	10	645	18	656	Probable alpha-galactosidase D OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=aglD PE=3 SV=2
sp|B8N7Z0|AGALD_ASPFN	7.38e-294	10	645	18	654	Probable alpha-galactosidase D OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=aglD PE=3 SV=1
sp|A1D9S3|AGALD_NEOFI	9.40e-293	13	645	20	646	Probable alpha-galactosidase D OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=aglD PE=3 SV=1
sp|A4DA70|AGALD_ASPFU	5.40e-292	13	645	20	646	Probable alpha-galactosidase D OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=aglD PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.995181	0.004861

TMHMM  Annotations     

There is no transmembrane helices in PMAA_096570-t26_1-p1.