CAZyme Information: PMAA_057160-t26_1-p1

Basic Information

• Species: Talaromyces marneffei
• Lineage: Ascomycota; Eurotiomycetes; ; Trichocomaceae; Talaromyces; Talaromyces marneffei
• CAZyme ID: PMAA_057160-t26_1-p1
• CAZy Family: GH2
• CAZyme Description: beta galactosidase, putative

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1009	DS995903|CGC10	111835.39	5.1148

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_TmarneffeiATCC18224	10194	441960	145	10049

• Gene Location: location=DS995903:1939710-1943022(-)

Full Sequence      

MAQLFTKIIVYFLLFASPLLANQWPLHNNSLNDVVQWDHYSFEINGQRLFVFAGEWHYWR
IPVPELWIDILEKIKAAGFTAFGIYVNWAYHAPNNYTVDFATGAHDITPILKMAKDVGLY
VLLRPGPYINAEVNAGGFPLWVTTGEYGSLRNDDSRYTAAWEPYFTKISEIASEYQITKG
GNVVTYQIENEFGDQWTGSPSRRVQYEPAAKYMELLEANARRNGIDIPLVANEPNMRAIS
WGKDWSNSSANVDVVGLDSYPSCWTCDLSVCTGTTGEYIAYQVIDYYGYFQETQPTMPSF
FAEFQGGSYNPWGGPVGGCPEDIGPDFANLFYRWNIGQRVTAINLYMLYGGTNWGAIAAP
VVASSYDYSSPISENRTIGAKYYETKLLTMFTRAARDLIVTDLIGNGTQYSTNPAIQTHV
IRNPITNGTFYVTLHTISSSSTDETFQLHVNTSAGAFSIPRYGNSIRLNGHQSKIIVTDF
QFGTHKLLYSTAEVLTYTVLDGIPTLALWVPTGESGEFSVLGGKWASVLRCEWCSDIQFH
PEQDQTSNPTVSRLTVSFTQGQGMSVIKLDTGLRVVLLDRESAYYFWAPALNSDPSVPED
QSVLVQGPHLVRSAKIVGSTIRLTGDSAQTSPIEVFAPKQVRIIFWNDKELKTSKTSYDS
LQASLPQPAYVKLPLLGPWKYNGSLPEKAQDYKDTSAAWISADHMKTSNPSPPATFPVLY
ADEYGFHNSIRIWRGYFTGNATGVVLKVQGGYAFGFSGWLNGKLLGSYLGNASVEHSHLT
LPFNVSHVSTSSDNVLVIVHDDTGHDETTGALNPRGILEATLLSDNSSSKFSHWRVAGTA
GGETNIDPMRGPYNEGGLYAERMGWHLPGFNDNAWSDAGSKLNFTGADIKFYRTTVPLNI
PKGVDVSISFELSACGTTNAFRSQLFVNGYQMGRFNPWVGNQIEFPVPPGILDYTGDNTI
GLSLWAQTEDGACAIVDWKINYVLGSSLDVTFNGEYLRPGWTSERLQYS

Enzyme Prediction     

EC	3.2.1.-:1	-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH35	42	388	3.6e-83	0.9869706840390879

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
396048	Glyco_hydro_35	6.71e-85	41	388	1	314	Glycosyl hydrolases family 35.
402180	BetaGal_dom2	2.47e-76	398	584	1	179	Beta-galactosidase, domain 2. This is the second domain of the five-domain beta-galactosidase enzyme that altogether catalyzes the hydrolysis of beta(1-3) and beta(1-4) galactosyl bonds in oligosaccharides as well as the inverse reaction of enzymatic condensation and trans-glycosylation. This domain is made up of 16 antiparallel beta-strands and an alpha-helix at its C-terminus. The fold of this domain appears to be unique. In addition, the last seven strands of the domain form a subdomain with an immunoglobulin-like (I-type Ig) fold in which the first strand is divided between the two beta-sheets. In penicillin spp this strand is interrupted by a 12-residue insertion which forms an additional edge-strand to the second beta-sheet of the sub-domain. The remainder of the second domain forms a series of beta-hairpins at its N-terminus, four strands of which are contiguous with part of the Ig-like sub-domain, forming in total a seven-stranded antiparallel beta-sheet. This domain is associated with family Glyco_hydro_35, pfam01301, which is N-terminal to it, but itself has no metazoan members.
198097	BetaGal_dom2	2.64e-53	397	586	1	182	Beta-galactosidase, domain 2. This is the second domain of the five-domain beta-galactosidase enzyme that altogether catalyses the hydrolysis of beta(1-3) and beta(1-4) galactosyl bonds in oligosaccharides as well as the inverse reaction of enzymatic condensation and trans-glycosylation. This domain is made up of 16 antiparallel beta-strands and an alpha-helix at its C terminus. The fold of this domain appears to be unique. In addition, the last seven strands of the domain form a subdomain with an immunoglobulin-like (I-type Ig) fold in which the first strand is divided between the two beta-sheets. In penicillin spp this strand is interrupted by a 12-residue insertion which forms an additional edge-strand to the second beta-sheet of the sub-domain. The remainder of the second domain forms a series of beta-hairpins at its N terminus, four strands of which are contiguous with part of the Ig-like sub-domain, forming in total a seven-stranded antiparallel beta-sheet. This domain is associated with family Glyco_hydro_35, which is N-terminal to it, but itself has no metazoan members.
166698	PLN03059	1.22e-37	4	374	2	323	beta-galactosidase; Provisional
404274	BetaGal_dom4_5	1.86e-37	859	970	1	111	Beta-galactosidase jelly roll domain. This domain is found in beta galactosidase enzymes. It has a jelly roll fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QGA20272.1|GH35	0.0	1	1009	1	1009
AOV94179.1|GH35	0.0	24	1009	29	1014
BCR96126.1|CBM67|GH35	0.0	24	1009	29	1015
AGS42423.1|GH35	0.0	22	1009	6	1001
BCS26099.1|GH35	0.0	22	1009	27	1036

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1TG7_A	9.26e-177	31	972	2	936	Native structure of beta-galactosidase from Penicillium sp. [Penicillium sp.],1XC6_A Native Structure Of Beta-Galactosidase from Penicillium sp. in complex with Galactose [Penicillium sp.]
5IFP_A	3.31e-170	25	971	36	971	Structure Of Beta-galactosidase From Aspergillus Niger [Aspergillus niger CBS 513.88]
4IUG_A	7.40e-170	25	972	36	970	Crystal structure of beta-galactosidase from Aspergillus oryzae in complex with galactose [Aspergillus oryzae]
5IFT_A	9.16e-170	25	971	36	971	STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 3-b-Galactopyranosyl glucose [Aspergillus niger CBS 513.88],5IHR_A Structure Of E298q-beta-galactosidase From Aspergillus Niger In Complex With Allolactose [Aspergillus niger CBS 513.88],5JUV_A STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 6-b-Galactopyranosyl galactose [Aspergillus niger CBS 513.88],5MGC_A STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 4-Galactosyl-lactose [Aspergillus niger CBS 513.88],5MGD_A STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 6-Galactosyl-lactose [Aspergillus niger CBS 513.88]
3OG2_A	9.83e-155	31	972	22	968	Chain A, Beta-galactosidase [Trichoderma reesei],3OGR_A Chain A, Beta-galactosidase [Trichoderma reesei],3OGS_A Chain A, Beta-galactosidase [Trichoderma reesei],3OGV_A Chain A, Beta-galactosidase [Trichoderma reesei]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|B6GW04|BGALB_PENRW	0.0	23	1009	28	1013	Probable beta-galactosidase B OS=Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) OX=500485 GN=lacB PE=3 SV=2
sp|B6QLF0|BGALB_TALMQ	0.0	1	1009	1	1009	Probable beta-galactosidase B OS=Talaromyces marneffei (strain ATCC 18224 / CBS 334.59 / QM 7333) OX=441960 GN=lacB PE=3 SV=1
sp|Q5BEQ0|BGALB_EMENI	0.0	22	989	27	1003	Probable beta-galactosidase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=lacB PE=3 SV=2
sp|Q4WRD3|BGALB_ASPFU	0.0	23	1009	28	1015	Probable beta-galactosidase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=lacB PE=3 SV=1
sp|B2W791|BGALB_PYRTR	0.0	24	1009	35	1009	Probable beta-galactosidase B OS=Pyrenophora tritici-repentis (strain Pt-1C-BFP) OX=426418 GN=lacB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000257	0.999699	CS pos: 21-22. Pr: 0.9810

TMHMM  Annotations     

There is no transmembrane helices in PMAA_057160-t26_1-p1.