CAZyme Information: PIW_T012990-RA-p1

Basic Information

• Species: Globisporangium iwayamae
• Lineage: Oomycota; NA; ; Pythiaceae; Globisporangium; Globisporangium iwayamae
• CAZyme ID: PIW_T012990-RA-p1
• CAZy Family: GT71
• CAZyme Description: Beta-glucan synthesis-associated protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
530		56991.08	7.1443

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_GiwayamaeDAOMBR242034	14874	1223558	0	14874

• Gene Location: location=PiwaDAOMBR242034_SC04704:226-1818(-)

Full Sequence      

MGNLGRAIFSASTNRMWPFSYDECNEKAFKSSNQRISACNANPGHGLNPNQGRGAPEIDI
LEGAGTEVSSSIQLGPGMPTDFRTMPPSGEAYGCIYSGDCKTPGANAPDLPTNLYKTKRG
HKSWYQGMRYAANNFCASSGKLKQDYKTVKAALDKGLVDNACTTATCPGSRDVNADVTGI
DGSTTAYWGINTNGTCFPLMNGYTGAYLCSPGNPSSACGASTIPQSDKVSVFEYQMDAIS
ANWPIHLAAYQDFLVYQIEWVTGPTGYVRWMLAGQPLYEVPASAIANPPQDASKTNPIKV
MIEEPMYMIMNVALSSTWGTKPPNAGGPCRGSTTDAASAKICDAFPMKMKIDYIRLYQDT
SPASKMQVGCDPASHPTKQWIVDHIDEYQDFDNLVVDVSGKAFCRTDDDCTGSKSKVSTG
SCVKSRCKCVSASWTGPRCTTSLGTVDTSDSSTGISIKSSYGPPWYLALGTSGLTILVTF
LAVYLSIQSAQKHEAERKKQTMMQQPKNAQSFGSASDVGMEKNNYSTNFV

Enzyme Prediction     

EC	-	-

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397841	SKN1	1.16e-27	1	390	222	478	Beta-glucan synthesis-associated protein (SKN1). This family consists of the beta-glucan synthesis-associated proteins KRE6 and SKN1. Beta1,6-Glucan is a key component of the yeast cell wall, interconnecting cell wall proteins, beta1,3-glucan, and chitin. It has been postulated that the synthesis of beta1,6-glucan begins in the endoplasmic reticulum with the formation of protein-bound primer structures and that these primer structures are extended in the Golgi complex by two putative glucosyltransferases that are functionally redundant, Kre6 and Skn1. This is followed by maturation steps at the cell surface and by coupling to other cell wall macromolecules.
185689	GH16_fungal_KRE6_glucanase	2.63e-20	1	358	104	295	Saccharomyces cerevisiae KRE6 and related glucanses, member of glycosyl hydrolase family 16. KRE6 is a Saccharomyces cerevisiae glucanase that participates in the synthesis of beta-1,6-glucan, a major structural component of the cell wall. It is a golgi membrane protein required for normal beta-1,6-glucan levels in the cell wall. KRE6 is closely realted to laminarinase, a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
185693	GH16_laminarinase_like	2.40e-11	243	357	150	235	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
185687	GH16_beta_agarase	0.007	252	358	180	258	Beta-agarase, member of glycosyl hydrolase family 16. Beta-agarase is a glycosyl hydrolase family 16 (GH16) member that hydrolyzes the internal beta-1,4-linkage of agarose, a hydrophilic polysaccharide found in the cell wall of Rhodophyceaea, marine red algae. Agarose is a linear chain of galactose units linked by alternating L-alpha-1,3- and D-beta-1,4-linkages that are additionally modified by a 3,6-anhydro-bridge. Agarose forms thermo-reversible gels that are widely used in the food industry or as a laboratory medium. While beta-agarases are also found in two other families derived from the sequence-based classification of glycosyl hydrolases (GH50, and GH86) the GH16 members are most abundant. This domain adopts a curved beta-sandwich conformation, with a tunnel-shaped active site cavity, referred to as a jellyroll fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UIZ28151.1|GH16	2.12e-204	1	530	205	752
UIZ28149.1|GH16	1.11e-201	1	530	204	754
UIZ28152.1|GH16	4.10e-198	1	530	193	739
UIZ24235.1|GH16_2	5.11e-198	1	439	205	655
UIZ27996.1|GH16	1.22e-193	1	502	206	699

PDB Hits     

PIW_T012990-RA-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P87023|KRE6_CANAX	6.94e-14	1	389	456	718	Beta-glucan synthesis-associated protein KRE6 OS=Candida albicans OX=5476 GN=KRE6 PE=2 SV=1
sp|P32486|KRE6_YEAST	1.11e-12	1	419	431	720	Beta-glucan synthesis-associated protein KRE6 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=KRE6 PE=1 SV=2
sp|P87024|SKN1_CANAX	5.16e-10	1	403	460	729	Beta-glucan synthesis-associated protein SKN1 OS=Candida albicans OX=5476 GN=SKN1 PE=2 SV=1
sp|P33336|SKN1_YEAST	6.97e-10	1	388	483	739	Beta-glucan synthesis-associated protein SKN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SKN1 PE=1 SV=1
sp|O13789|YEOB_SCHPO	1.62e-07	1	391	348	615	Uncharacterized beta-glucan synthesis-associated protein C17G6.11c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC17G6.11c PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000057	0.000000

TMHMM  Annotations     

Start	End
465	487