CAZyme Information: PIW_T006459-RA-p1

Basic Information

• Species: Globisporangium iwayamae
• Lineage: Oomycota; NA; ; Pythiaceae; Globisporangium; Globisporangium iwayamae
• CAZyme ID: PIW_T006459-RA-p1
• CAZy Family: GH5
• CAZyme Description: Alpha,alpha-trehalose-phosphate synthase [UDP-forming]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1236		138621.36	4.6777

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_GiwayamaeDAOMBR242034	14874	1223558	0	14874

• Gene Location: location=PiwaDAOMBR242034_SC00952:3602-7312(-)

Full Sequence      

MTEAAHSPPPMDTDVVLDVSFKDREKDLLQELAALYSKLNQVREKRVKAEEESSRGNSNH
ANGGSMADDDESTTSGKSRKRRRVLLVTRSLPFTLVWEDAQSQWRAEFPDNVSLDGAMET
FMSMHEHYDCVWIGSVHGDIEPSEQNSLKEQLLEDRNYFPVFLDPKRERLFYNGFCKTIM
WPLFHSCPPTTDDQLSTHTEMDTSSYGEDDFDMEKMWQAYVGANQAFADAVQEVYEEGDL
VWIQGYHLTLVPQMVQNLFPNDNIDIGYFLHIPFPSSELYRIMNNREEILTGMLGADLIG
FQTYEYARHFQSSAVRLLGLESSHKGVEYNGHFARVTICPVGIDADKYTALVSTNNVQEQ
LKQMTNQFAGKKVILGVDPLDLTKGLVHKLLAVEELFTLHPELSKEVVFLQIVTGTPSLF
ESPLGSQVESLVSRINSKLQDIGADGPVQYLNKEIPQEALIALYAIADTLVVTPVRDGMN
TIPFEYVVCREAHGKHARVVLSEFAGSAHSLGGAVLVNPWNTEELLDALLASLQENDDVM
RAHQSMYSYVRTFTSRHWVDNFLEQLRECSEENGLAVSGRELSRRDMTSAYIRSSRRLIF
ITYEGVLAAEASIPELSYPPQELIWQLSMLTKDSRNTVVLVCSRSTAVCEQWFAGLTGNI
VLAAEYGVYVKWVGEHEYWHCMVPNMDMSWWEHVVPLLEYYTERTPGAFIERKDSSVAWH
YRDCDLDHGLWQASELLVSLRELTRSLPVAVCPGNRVLEVRPQKASKAWLFERIWEFMNY
TILFPDEDPVTTDYFGDPVKPLSSPLVKPWAGAGGNNWRKDTPHLTPIHLAAIVNADANE
SGRKESGEREEHGSDDDERVTSDDKHQPVDFLLVIASGEDRTDEDLFAYLVPAPIDLEAY
CRQLEKDQVDDEDSQRFSHNDAGVTDSVSSGNFDDSTKRLSPRGSFRRLLGLDSSSSNAE
SSGEPLPSPLPRIPSQGADSMDILLLGENITKLTPLYAHSSKQAISDDAANMFPRTLLNN
PSNARRDEITERSKFTPRRDFPPSAALFKAMKSKYGENFSMHRLPDTPAAAWALVVPPNA
RAKLEDSNATTPLISSSLGPGGLDRKSSFTSSTAVMAGSPISSSAHEGGGTAGSVTSDSY
SPSSSMVDGDEQMRREIEVLRSLDEDVTVRNEDHDEVEGPYRFPVNTFVCTLGRKLSQAP
YFIKNSADLYQLLQEMGLNSHIRKQRMMSMGSHMDE

Enzyme Prediction     

EC	2.4.1.15:146

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT20	76	567	2.2e-147	0.9831578947368421

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
184712	PRK14501	0.0	83	767	2	658	putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional
340820	GT20_TPS	2.09e-165	83	567	1	463	trehalose-6-phosphate synthase. Trehalose-6-Phosphate Synthase (TPS, EC 2.4.1.15) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain.
395781	Glyco_transf_20	7.33e-150	131	566	47	468	Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex,.
215556	PLN03064	6.59e-149	24	865	23	874	alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional
215555	PLN03063	1.46e-142	80	774	9	686	alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CCA15235.1|GT20	0.0	22	1229	28	1218
UIZ28038.1|GT20	0.0	20	1232	6	1241
QOX06485.1|GT20	5.83e-157	17	784	11	760
AJA04606.1|GT20	1.20e-153	84	774	130	806
AGT80036.1|GT20	1.20e-153	84	774	130	806

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5HVM_A	2.24e-93	84	570	14	478	Structure of Aspergillus fumigatus trehalose-6-phosphate synthase A in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293],5HVM_B Structure of Aspergillus fumigatus trehalose-6-phosphate synthase A in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293]
5HUV_A	3.84e-93	84	575	6	476	Structure of Candida albicans trehalose-6-phosphate synthase E341R/E346R in complex with UDP-glucose [Candida albicans SC5314],5HUV_B Structure of Candida albicans trehalose-6-phosphate synthase E341R/E346R in complex with UDP-glucose [Candida albicans SC5314]
5HUT_A	5.30e-93	84	575	6	476	Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP-glucose [Candida albicans SC5314],5HUT_B Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP-glucose [Candida albicans SC5314],5HUU_A Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and glucose-6-phosphate [Candida albicans SC5314],5HUU_B Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and glucose-6-phosphate [Candida albicans SC5314],5HVL_A Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and validoxylamine A [Candida albicans SC5314],5HVL_B Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and validoxylamine A [Candida albicans SC5314]
5HVO_A	2.61e-91	84	570	13	478	Structure of Aspergillus fumigatus trehalose-6-phosphate synthase B in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293],5HVO_B Structure of Aspergillus fumigatus trehalose-6-phosphate synthase B in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293],5HVO_C Structure of Aspergillus fumigatus trehalose-6-phosphate synthase B in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293],5HVO_D Structure of Aspergillus fumigatus trehalose-6-phosphate synthase B in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293]
6JBI_A	4.08e-89	84	566	2	462	Structure of Tps1 apo structure [Pyricularia oryzae 70-15],6JBI_B Structure of Tps1 apo structure [Pyricularia oryzae 70-15],6JBR_A Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_B Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_D Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_F Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_H Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_K Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_M Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_O Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBW_A Structure of Tps1/UDP complex [Pyricularia oryzae 70-15],6JBW_B Structure of Tps1/UDP complex [Pyricularia oryzae 70-15]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q54K57|TPSA_DICDI	8.56e-144	84	786	18	690	Alpha,alpha-trehalose-phosphate synthase [UDP-forming] A OS=Dictyostelium discoideum OX=44689 GN=tpsA PE=2 SV=1
sp|Q54NU9|TPSB_DICDI	4.57e-131	117	795	83	747	Alpha,alpha-trehalose-phosphate synthase [UDP-forming] B OS=Dictyostelium discoideum OX=44689 GN=tpsB PE=3 SV=1
sp|Q0WUI9|TPS8_ARATH	3.82e-124	85	774	60	760	Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] 8 OS=Arabidopsis thaliana OX=3702 GN=TPS8 PE=2 SV=1
sp|Q9LRA7|TPS9_ARATH	1.35e-123	85	774	62	765	Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] 9 OS=Arabidopsis thaliana OX=3702 GN=TPS9 PE=2 SV=1
sp|Q9SYM4|TPS1_ARATH	2.93e-120	84	774	94	774	Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1 OS=Arabidopsis thaliana OX=3702 GN=TPS1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000066	0.000000

TMHMM  Annotations     

There is no transmembrane helices in PIW_T006459-RA-p1.