CAZyme Information: PIW_T005890-RA-p1

Basic Information

• Species: Globisporangium iwayamae
• Lineage: Oomycota; NA; ; Pythiaceae; Globisporangium; Globisporangium iwayamae
• CAZyme ID: PIW_T005890-RA-p1
• CAZy Family: GH35
• CAZyme Description: Choline dehydrogenase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
591		65274.47	7.1632

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_GiwayamaeDAOMBR242034	14874	1223558	0	14874

• Gene Location: location=PiwaDAOMBR242034_SC00823:11565-13340(-)

Full Sequence      

MLRHSLRTSAAARSPLASSSSSATRALSTSPAAAAEEYDYVIVGAGSAGCVLANRLSAEP
GNKVLLVETGPSDINKWDSWKIQMPAALTFNLGDDKYNWAYHTEPQKNLDNRRLPWPRGR
VLGGSSSLNAMVYIRGHAFDYDDWQASGADGWSYADCLPYFRKAQTHELGPDEYRGGDGP
LHVTRGSQKDQVLFKKFIEAGIQAGYPYTDDMNGYQQEGFGWMDSTVHKGKRWSAASGYL
HPVTSRKNLTVITDTFVNKIIFEGKRAVGIDTENNKHKTTAQIRATKEIILSGGAINSPQ
LLMLSGVGDAKHLKEMDIPVVQNLPAVGQNMEDHLDLYIQYRCKKPITLHNATWKYPHNM
VKIGLEWFAMQTGMGASAHLESGGFIRSAPGKCHPDLQYHFLPGSLTGQLTPGSYHAMQA
HCSPMRATSRGFLKLRSTNPRDYPIIEPNYLDTEEDKVDIRAGVRLTREIFEQQVFDEFR
GDAVSPAQNVQSDAEIDAWVRQHSESAYHPSCTNRMGVDDNTVVDPQTRVHGLENLRIVD
ASIMPNIVSGNLNGPTIMLAEKAADIILGNAALAKSKAPVYQPVNWESSQR

Enzyme Prediction     

No EC number prediction in PIW_T005890-RA-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
AA3	37	568	1.3e-167	0.9929577464788732

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
273814	betA	0.0	39	573	1	532	choline dehydrogenase. Choline dehydrogenase catalyzes the conversion of exogenously supplied choline into the intermediate glycine betaine aldehyde, as part of a two-step oxidative reaction leading to the formation of osmoprotectant betaine. This enzymatic system can be found in both gram-positive and gram-negative bacteria. As in Escherichia coli, Staphylococcus xylosus, and Sinorhizobium meliloti, this enzyme is found associated in a transciptionally co-induced gene cluster with betaine aldehyde dehydrogenase, the second catalytic enzyme in this reaction. Other gram-positive organisms have been shown to employ a different enzymatic system, utlizing a soluable choline oxidase or type III alcohol dehydrogenase instead of choline dehydrogenase. This enzyme is a member of the GMC oxidoreductase family (pfam00732 and pfam05199), sharing a common evoluntionary origin and enzymatic reaction with alcohol dehydrogenase. Outgrouping from this model, Caulobacter crescentus shares sequence homology with choline dehydrogenase, yet other genes participating in this enzymatic reaction have not currently been identified. [Cellular processes, Adaptations to atypical conditions]
225186	BetA	0.0	31	574	1	540	Choline dehydrogenase or related flavoprotein [Lipid transport and metabolism, General function prediction only].
235000	PRK02106	0.0	34	591	2	555	choline dehydrogenase; Validated
366272	GMC_oxred_N	4.16e-59	110	336	15	218	GMC oxidoreductase. This family of proteins bind FAD as a cofactor.
398739	GMC_oxred_C	3.13e-44	428	563	2	143	GMC oxidoreductase. This domain found associated with pfam00732.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UIZ28087.1|AA3_2	0.0	1	591	1	581
ANI26486.1|AA3_2	2.70e-240	22	583	50	617
CAB3230510.1|AA3_2	6.12e-240	34	590	21	578
AWP09430.1|AA3_2	4.81e-238	38	583	20	566
CEF60566.1|AA3_2	2.10e-195	40	573	35	574

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6ZH7_A	1.77e-92	35	578	5	571	Chain A, Fatty acid photodecarboxylase, chloroplastic [Chlorella variabilis],6ZH7_B Chain B, Fatty acid photodecarboxylase, chloroplastic [Chlorella variabilis]
6YRU_AAA	2.48e-92	34	578	4	571	Chain AAA, Fatty acid Photodecarboxylase [Chlorella variabilis],6YRV_AAA Chain AAA, Fatty acid Photodecarboxylase [Chlorella variabilis],6YRX_AAA Chain AAA, Fatty acid Photodecarboxylase [Chlorella variabilis],6YRZ_AAA Chain AAA, Fatty acid photodecarboxylase, chloroplastic [Chlorella variabilis]
5NCC_A	2.67e-92	28	578	15	587	Structure of Fatty acid Photodecarboxylase in complex with FAD and palmitic acid [Chlorella variabilis],5NCC_B Structure of Fatty acid Photodecarboxylase in complex with FAD and palmitic acid [Chlorella variabilis],5NCC_C Structure of Fatty acid Photodecarboxylase in complex with FAD and palmitic acid [Chlorella variabilis],5NCC_D Structure of Fatty acid Photodecarboxylase in complex with FAD and palmitic acid [Chlorella variabilis],5NCC_E Structure of Fatty acid Photodecarboxylase in complex with FAD and palmitic acid [Chlorella variabilis],5NCC_F Structure of Fatty acid Photodecarboxylase in complex with FAD and palmitic acid [Chlorella variabilis]
6YS1_AAA	3.49e-92	34	578	4	571	Chain AAA, Fatty acid Photodecarboxylase [Chlorella variabilis]
6YS2_AAA	4.89e-92	34	578	4	571	Chain AAA, Fatty acid Photodecarboxylase [Chlorella variabilis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q8NE62|CHDH_HUMAN	4.38e-244	1	583	4	587	Choline dehydrogenase, mitochondrial OS=Homo sapiens OX=9606 GN=CHDH PE=1 SV=2
sp|Q8BJ64|CHDH_MOUSE	4.41e-242	36	583	41	589	Choline dehydrogenase, mitochondrial OS=Mus musculus OX=10090 GN=Chdh PE=1 SV=1
sp|Q6UPE0|CHDH_RAT	1.14e-240	36	583	44	592	Choline dehydrogenase, mitochondrial OS=Rattus norvegicus OX=10116 GN=Chdh PE=1 SV=1
sp|C3K3D3|BETA_PSEFS	1.33e-196	36	581	3	547	Oxygen-dependent choline dehydrogenase OS=Pseudomonas fluorescens (strain SBW25) OX=216595 GN=betA PE=3 SV=1
sp|Q88AE7|BETA_PSESM	1.11e-195	37	581	6	549	Oxygen-dependent choline dehydrogenase OS=Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000) OX=223283 GN=betA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000071	0.000001

TMHMM  Annotations     

There is no transmembrane helices in PIW_T005890-RA-p1.