CAZyme Information: PIW_T004570-RA-p1

Basic Information

• Species: Globisporangium iwayamae
• Lineage: Oomycota; NA; ; Pythiaceae; Globisporangium; Globisporangium iwayamae
• CAZyme ID: PIW_T004570-RA-p1
• CAZy Family: GH16|GH16
• CAZyme Description: Beta-mannosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
952		106871.56	4.9707

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_GiwayamaeDAOMBR242034	14874	1223558	0	14874

• Gene Location: location=PiwaDAOMBR242034_SC00561:12426-15690(-)

Full Sequence      

MLLRRAATTALLLLLSSACGAQKLPLTTWSLRSQSGSVRVENATVPGTSHTHLLDAGVIQ
DPYLRFNEREYQWIAKETWVYETHVTVGESDALAKSRLVFETLDGVAQVYVNDKLLVATA
NSFLSYAFGVENVLVRGRNAVKVVFTPPLDYASNKAQMTPYFVPATQNFNTWAEPTHRSF
IRKAGSDFGWDWGPAFATTGIAGVVHLELGASSPELANLDVMQQFPGGAANLSVVELAVK
ISLDGKGLQRDNVTFDLLVNGVLQSTKIASVCSTSDVEEVELLYTLNNPTLWWPSGYGNP
YLYELKVNARNEHFSASASHKIGIRHVVLVQEPTAAGNTTGATFYFKVNHVPIFIKGANW
IPTDSFPTRVQQSTVKNLIRSAFEANMNMIRVWGGGRYESDFFYSECDRLGLLVWQELMF
ACGMYPRDSDFLDLVSDEVRMQVKRLRKYTSIAIWGGNNENESMMEQFADGDFMPLNTPF
NRDIAVADYTKLFVDVIYPIFTSLDPSRPFVDTSPSNGLYSVDPFVKRWGPSNGIAYGDV
HFYDYASDCQQSETFPHARFISEFGFQSAPSLTSLREVGSDADWESFQSFWTFLKFRERH
ANGTAEMLHQMQRRFVIPFPFSDEEWLGIPYSRQVRDISGALGFNISKRIDSYLYLTQIQ
QGLCYQTAIRTWRRGKNVDLGMTMGILYWQLNDIWQGTSWSSVEFSGRWKSLHNFVKREF
APFVLSIHQQNTTGAVEVYGVSDIQQDLEIEVVYELRSTRNGSTLKTTTDNIVLSPLTVF
YGFTDALCLQESKQVWRVDVDEILDETATSCTVTSCFLYVQCHLKSPSSLAAQALCSDVN
HFFAPYKSLDLGKGGIEIVGVQESSASPASFDVTIRSSSFVALFVEVETPSSHGFWSENS
FLMLANQEKVLTFTRFDADGGEAGQPSIQEFKESLSVKWLQKIYEDDIMSVE

Enzyme Prediction     

No EC number prediction in PIW_T004570-RA-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	41	791	3e-85	0.660904255319149

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
225789	LacZ	1.36e-64	29	713	19	623	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
407625	Ig_mannosidase	5.84e-09	865	938	9	74	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
395572	Glyco_hydro_2	4.50e-08	235	325	18	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
407658	Mannosidase_ig	6.80e-07	734	847	1	90	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
236673	ebgA	8.33e-06	236	460	228	449	cryptic beta-D-galactosidase subunit alpha; Reviewed

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CCA25641.1|GH2	2.70e-263	26	952	62	999
AIG55460.1|GH2	2.50e-236	22	951	24	871
BDA42312.1|GH2	6.03e-148	15	944	19	921
AAD39352.1|GH2|3.2.1.25	8.94e-147	29	913	29	856
AAC48665.1|GH2|3.2.1.25	1.15e-146	29	913	29	856

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6DDT_A	6.21e-142	28	913	17	845	mouse beta-mannosidase (MANBA) [Mus musculus],6DDU_A mouse beta-mannosidase bound to beta-D-mannose (MANBA) [Mus musculus]
7OP6_A	1.55e-105	27	938	15	843	Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2VJX_A	2.81e-105	27	934	13	837	Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	2.93e-105	27	934	15	839	Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	3.99e-105	27	938	13	841	Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q95327|MANBA_CAPHI	2.05e-147	29	913	29	856	Beta-mannosidase OS=Capra hircus OX=9925 GN=MANBA PE=1 SV=1
sp|Q29444|MANBA_BOVIN	5.66e-147	29	913	29	856	Beta-mannosidase OS=Bos taurus OX=9913 GN=MANBA PE=1 SV=1
sp|Q4FZV0|MANBA_RAT	8.30e-142	15	913	8	858	Beta-mannosidase OS=Rattus norvegicus OX=10116 GN=Manba PE=2 SV=1
sp|Q8K2I4|MANBA_MOUSE	4.26e-141	28	913	28	856	Beta-mannosidase OS=Mus musculus OX=10090 GN=Manba PE=1 SV=1
sp|O00462|MANBA_HUMAN	2.45e-139	29	913	29	856	Beta-mannosidase OS=Homo sapiens OX=9606 GN=MANBA PE=1 SV=3

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000632	0.999354	CS pos: 18-19. Pr: 0.8625

TMHMM  Annotations     

There is no transmembrane helices in PIW_T004570-RA-p1.