CAZyme Information: PITG_13567-t26_1-p1

Basic Information

• Species: Phytophthora infestans
• Lineage: Oomycota; NA; ; Peronosporaceae; Phytophthora; Phytophthora infestans
• CAZyme ID: PITG_13567-t26_1-p1
• CAZy Family: GH63
• CAZyme Description: endo-1,3(4)-beta-glucanase, putative

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
899	DS028146|CGC3	97127.38	5.0637

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PinfestansT30-4	19344	403677	1547	17797

• Gene Location: location=DS028146:1050349-1053048(+)

Full Sequence      

MKLTQWVRVAAVIATATASAVAFDLSLLDDDTNYRKGRLHAPQEIEEERAASAGGSLTQT
SVDAATSTSASTSTSFSSSGPDGTLAGSALSFTSNSAATSSDSGSDAGLSSSSLAAALSN
SGSDTSLPNSGSDASLSMSGSEDSASADTSTSSSTDTLSASESDAASTSGSGDSPVASST
GSSATQNTGGGDFLAPFSTEEPSTTLFPRSALGEMAPIINVAEGLLGKPLPTNKWWGNII
HTTAEEMDTKANPGWSNPYAVKLPKEAPYGIQACYSYNYRQLSALTDGVAEFYLHDFVND
LTLSATEFAGEAKPTYEIYAFSDFGINVRACLENKVQCLDSALVHGMAFITATYDRLTAR
IESEYTMEIVDKSVPGKYILQLGGNQTWVVYTGNNGSFALAESGKALESSGLFSGTVRVA
ILPSKKATTVYDKYRACHVRGGNVSVESRTQYSLNWETVGKSCKTNGMLHFALPHHLPAL
KGAITAKSPKAIVLNSATRGKMVAQVATTGKWVLSEPEDELEVDFYPTSKPSAEVVAKVG
LLQTLQADIADHWALNKTSWYFNGKQYQKYASVCLMAADSAIVGKDKKLLSACLTKLEKL
LVPFLDNTLDPPLHYETSYGGLVSSQSFTVKDVNADFGNSVYNDHHYHYGYWVTASAMLK
SLHPKWERIKELDRMIWMMLRDVANPSTDDPFFPQFRHFSFYLGHSYSHGVTPMLDGKDE
ESTSEDVNFYYGMKLWGQVSSNKAVEDLGSLMLRLNARAIRTYFLMTSDNTIHPTEFVPN
HVTGIFFDNKAAYATWFSGEKYAIHGIQMIPVSPINAMVRTTKFVQQEWDDILSKQPIVT
ESNTSNAWLSLLLVNQAAVDQEDALTKLQEATMDDGLTRSWALYNAASRPHNAKQVDVA

Enzyme Prediction     

No EC number prediction in PITG_13567-t26_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH81	223	884	1.4e-169	0.977491961414791

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
407548	Glyco_hydro81C	4.47e-137	529	883	3	349	Glycosyl hydrolase family 81 C-terminal domain. Family of eukaryotic beta-1,3-glucanases. Within the Aspergillus fumigatus protein, two perfectly conserved Glu residues (E550 or E554) have been proposed as putative nucleophiles of the active site of the Engl1 endoglucanase, while the proton donor would be D475. The endo-beta-1,3-glucanase activity is essential for efficient spore release. This entry represents the helical C-terminal domain.
227785	Acf2	3.34e-127	166	887	14	753	Endoglucanase Acf2 [Carbohydrate transport and metabolism].
397619	Glyco_hydro_81	2.13e-44	227	527	12	318	Glycosyl hydrolase family 81. Family of eukaryotic beta-1,3-glucanases. Within the Aspergillus fumigatus protein ENGL1, two perfectly conserved Glu residues (E550 or E554) have been proposed as putative nucleophiles of the active site of the Engl1 endoglucanase, while the proton donor would be D475. The endo-beta-1,3-glucanase activity is essential for efficient spore release.
226277	RgpF	0.003	649	810	63	208	Lipopolysaccharide biosynthesis protein [Cell wall/membrane/envelope biogenesis].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UIZ26438.1|GH81	0.0	190	889	41	740
UIZ26440.1|GH81	0.0	190	889	41	740
UIZ26131.1|GH81	6.07e-289	193	889	12	714
UIZ26137.1|GH81	4.65e-286	193	889	122	824
UIZ26602.1|GH81	4.65e-286	193	889	122	824

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4K3A_A	1.89e-92	190	891	5	720	The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei],4K3A_B The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei]
5XBZ_A	2.32e-92	185	891	24	745	Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminaripentaose [Rhizomucor miehei],5XBZ_B Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminaripentaose [Rhizomucor miehei],5XC2_A Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei],5XC2_B Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei],5XC2_C Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei],5XC2_D Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei]
4K35_A	9.22e-88	190	891	5	720	The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei],4K35_B The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei]
6FOP_A	3.42e-26	204	833	11	603	Chain A, Glycoside hydrolase family 81 [Acetivibrio thermocellus ATCC 27405]
5V1W_A	2.72e-22	526	875	354	664	Chain A, Glycoside Hydrolase [Halalkalibacterium halodurans C-125]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q09850|ENG2_SCHPO	1.16e-93	192	887	2	700	Ascus wall endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng2 PE=1 SV=1
sp|Q9UT45|ENG1_SCHPO	7.86e-84	205	887	54	735	Primary septum endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng1 PE=3 SV=1
sp|D4AZ24|ENG1_ARTBC	3.89e-83	229	887	225	900	Probable endo-1,3(4)-beta-glucanase ARB_01444 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_01444 PE=1 SV=1
sp|Q12168|ENG2_YEAST	1.27e-81	229	883	110	772	Endo-1,3(4)-beta-glucanase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ACF2 PE=1 SV=1
sp|Q5AIR7|ENG1_CANAL	4.07e-79	185	887	420	1139	Endo-1,3(4)-beta-glucanase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=ENG1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.004540	0.995424	CS pos: 22-23. Pr: 0.9434

TMHMM  Annotations     

There is no transmembrane helices in PITG_13567-t26_1-p1.