CAZyme Information: PITG_10743-t26_1-p1

Basic Information

• Species: Phytophthora infestans
• Lineage: Oomycota; NA; ; Peronosporaceae; Phytophthora; Phytophthora infestans
• CAZyme ID: PITG_10743-t26_1-p1
• CAZy Family: GH30
• CAZyme Description: conserved hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1719		190073.62	7.2009

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PinfestansT30-4	19344	403677	1547	17797

• Gene Location: location=DS028137:952266-959190(+)

Full Sequence      

MSDEFNVANRSFRPGDDHMWTSLDKPDGVNGALEIYAHNMTSTKCDDDGTCYFYIEVDSD
NYTISVYNMYTHPPGYMNSTFYYRAAMVQSWNKFCFQGGMLEVRAQLPGAVSTASGNPDL
ALGKNGQVTETSYYPTWPGIWMMGNLGRAIFSGSTNRMWPFSYDRCEPEIFDPSNQRISA
CDDDPGYGMNPNQGRGAPEIDLLEGGGLAISSSLQIAPGMPTDFRLFPADAKGVDATNPY
CVYAYNCKTDGANLIDVPTDFYEQERGHKSWYQGLRYAANNNCAKKKANVQNFATVNASV
ARGISENSCTTTTCPASLDVNGDLDFIDGTGKNHWGINSNGTCFPIMNSYMGAYLCDPDN
TNARCTSPRNSTTPKSNAMDTFNYQMDAISANWPIHVGAYTGYLVYQLEWVTGVNGYARW
MLQGSPLFEVTADSFSNVPQNSNSSNPQKVMLEEPMSIIVNVALSSSWGTKPPNPGTACR
GDGSDKTVNKICDEFPMFMKVDYIRLYQDMGDDLEADNYMQVSCDPASHPTKEWIQGHID
EYQDDDNLATEVIVWRGVSAAAKRNAAVTKANMMAKGDLETRVAGNRNSSDVSHFVDLVM
LGIKAFVQESVHALPPSQLRLLAETLRVDLRGCEDRRDIERVLTGLVCLDQDTSLGVFLA
WLRSADLQQHKNASKKQTFNNTFLPAITETRPTNQQSERVKDHHLDDEITQHLRELEALE
ASFKKAERLVHTGSPSFEKLKRFLMELTLLRAKEQSARVFLVQQVSVLKQQHADMRAEVL
HSRAQLDFFVEGFTNLRKRHDALLTDATRMKAENESAHEIFVSMSAHDCFFEQLMRNTLR
QQMRDNDEVTRRLQQATEDLDAAARKRRELEDQIAQLKQERGGARKDAYCFKRQLRVCKT
RMRRLQTGGGDGSFFRDQAVELRQTVATLLGLLRDAVVRDTKSPKQALSKEALRILHQVL
TPKIKLPESEAHFTRFHGNQEPASGEIDPTQPRVVYRQATLDIDEVVKGVLLVEGSSYAS
ALQKHVSALMAAQNKRGFVMYNWPFSSRDVHLLTAIGLPVDSVINLEVIESAPTSSSSIA
TSASTLAATSTPTPALKPKTPTVTTKAPPVSGSNSRSKAPVVSSTVRGKVSPTKPTAASK
SPVKPATRTAAPSAAKSRATSPTRGTKATIATPNTPSKPKTPTVPLTPVKSGTSSSQLKP
EATKVVKKIPTAASSLQGLGGILQSVAPAAFPAERVDAILRILEQQKRCRLAPVIRWDEA
TRHTNEMLAMWAEEVHMLVHLEQESRKPQKAPPTAAAIPAKTTPSQSERATVAAKSPQLQ
SKVSQKTNGDASSAVSPSAMRTHYYTRGLEPWCDHVSGRVGYTGDDSSDTSFQSNDGLVI
YSPRKTSITQSDAEADPVVNQTTGHVSVAMEMQEGAAELADWVFAWRWQWICYGFFSTRA
RQLSRLERLTLLNVRVRTEDAEAMRAVMLSQCPEEDLLGLGQQEQQPEPREFSLKANASL
RQQSMDADDKDLGGNASLSVSREIRGVKRLGTQVDKGWVDVLVPGFGKCQTRRSNLVHNE
ATPVEDSKGLSLMLTFVEDPDDELMCEFLGVIGGSLRDLTIRVSNFRSAMLERITATCPR
LTEIVVCTPMLEARFQVRGNRLHHPIIVPSTVPSSFENTIDLVRSFSMTDPVARALRRLR
VCFRINYGEATTRPPLEECCTALPGMLNVNRTLYAHSTA

Enzyme Prediction     

EC	-	-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	3	227	1.4e-61	0.6073619631901841

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397841	SKN1	3.35e-58	2	544	122	478	Beta-glucan synthesis-associated protein (SKN1). This family consists of the beta-glucan synthesis-associated proteins KRE6 and SKN1. Beta1,6-Glucan is a key component of the yeast cell wall, interconnecting cell wall proteins, beta1,3-glucan, and chitin. It has been postulated that the synthesis of beta1,6-glucan begins in the endoplasmic reticulum with the formation of protein-bound primer structures and that these primer structures are extended in the Golgi complex by two putative glucosyltransferases that are functionally redundant, Kre6 and Skn1. This is followed by maturation steps at the cell surface and by coupling to other cell wall macromolecules.
185689	GH16_fungal_KRE6_glucanase	1.47e-52	2	508	4	295	Saccharomyces cerevisiae KRE6 and related glucanses, member of glycosyl hydrolase family 16. KRE6 is a Saccharomyces cerevisiae glucanase that participates in the synthesis of beta-1,6-glucan, a major structural component of the cell wall. It is a golgi membrane protein required for normal beta-1,6-glucan levels in the cell wall. KRE6 is closely realted to laminarinase, a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
225182	BglS	5.32e-08	82	167	102	157	Beta-glucanase, GH16 family [Carbohydrate transport and metabolism].
185693	GH16_laminarinase_like	9.87e-08	380	507	137	235	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
411345	gliding_GltJ	1.38e-07	1087	1215	409	530	adventurous gliding motility protein GltJ. Adventurous gliding motility protein GltJ, also known as AgmX, occurs in delta-proteobacteria such as Myxococcus xanthus.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UIZ27810.1|GH16	0.0	1	553	98	650
UIZ28149.1|GH16	2.12e-302	1	552	62	610
UIZ28151.1|GH16	1.28e-297	1	552	63	613
UIZ28152.1|GH16	2.12e-266	1	581	51	623
UIZ28150.1|GH16	2.90e-258	1	552	1	548

PDB Hits     

PITG_10743-t26_1-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P32486|KRE6_YEAST	3.02e-29	2	550	331	694	Beta-glucan synthesis-associated protein KRE6 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=KRE6 PE=1 SV=2
sp|P33336|SKN1_YEAST	1.97e-28	2	542	383	739	Beta-glucan synthesis-associated protein SKN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SKN1 PE=1 SV=1
sp|P87023|KRE6_CANAX	4.57e-23	2	544	356	719	Beta-glucan synthesis-associated protein KRE6 OS=Candida albicans OX=5476 GN=KRE6 PE=2 SV=1
sp|P87024|SKN1_CANAX	5.97e-23	2	544	360	716	Beta-glucan synthesis-associated protein SKN1 OS=Candida albicans OX=5476 GN=SKN1 PE=2 SV=1
sp|O13941|YEPB_SCHPO	2.70e-21	2	552	243	613	Uncharacterized beta-glucan synthesis-associated protein C23H3.11c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC23H3.11c PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.999675	0.000370

TMHMM  Annotations     

There is no transmembrane helices in PITG_10743-t26_1-p1.