CAZyme Information: PITG_07201-t26_1-p1

Basic Information

• Species: Phytophthora infestans
• Lineage: Oomycota; NA; ; Peronosporaceae; Phytophthora; Phytophthora infestans
• CAZyme ID: PITG_07201-t26_1-p1
• CAZy Family: GH16
• CAZyme Description: UDP-glucose:glycoprotein glucosyltransferase, putative

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1632		183754.63	4.9589

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PinfestansT30-4	19344	403677	1547	17797

• Gene Location: location=DS028127:2904123-2909205(-)

Full Sequence      

MRPTTWLSAGLAALLTAAQPSLTSARGVHVNLTASWPSSPFFPLMETSEFLAEENPLYFW
QFLEQLEARTSYVDLMNSDVDALGTLAVTVAESIAPGSKNVLELMLATRTYSVKVEMFRQ
LGLDSGIRPCGEGADTWAVFYKEKHCVESVACSEDELDAILRGKKQQTNDQACVAAGVND
VELQVDHKYPYIASDSENAPISAILYGLVGTEKFHAFHTKLVKQAQINKIQYMGYGVALD
IKNMEYTTIDDSKKTDSDDTTSDETGSEEEDEEEEGEIDDEEVDGFLFKPLMARHGAIAN
DLKEFYDILVQKADGEQEQELKAWHLKDLGATAVRHIVDAKNPLKRLETLSQDFPVQAKK
LAFSRKSISAKLREEIAVTRMQAANRRVRNKFIMNGIAVDPTERSFNVFDFMKTLKEEWS
VAKQLDSLPLNQSELEDMLKHVRETNQEQPAVRIHVRGAMGGSTPLYLNNIETDPNSASW
SPDVNILRRPAWNLIFVRKNMYECVLVMDPLSGAGRAALSHIGFMRMRGAPVQWALLISS
KELMASNTAEERQAVVEKYKAFKTTDKASSWHFAKLLMLAQSKDTAEAAAKDETSKSSQG
DDTEVKEEEERILPTRVASGFLQRVGEDEKSDVLVERLVEAYAEAAGGVGTTEDNEEEAL
ECLKSDQFDSDILAMTEFIHLKHLPLDSFVFNGVVQKDLDIQRSMMASFGRDQPLYVNMA
HRGLLTDDMDLVEELMTEQDAYPAYLSIFESSEARPGETEVKAPGHLVADDVDGRLEAAS
RPAVSYLHAPGSRTLPKKQTLIFPVDLNDPRDAGHAYRVVKAVLEDSEQSLRVGVVSQLK
DVEKNSVGELAAAIIATIGHSDKEAHLKFVLEALNCIVKHKSINTMKDKLRGIWKKTANS
ESDTDPVYEKVLKLLNSKTGKWLTKKQRGVLTQFNTLLRSRFPPAYVESEEDVPKVALPH
LFINGRQVTLPSQSLSDEDMATIVNFDLKYRTQPVAKALIKRSVTMNAKEADKLSFAIMK
TTGIVDKYVKTDRTPHMTVDENSLNTVRLSGDPSLQVAAYVDPLSEAAQVMSSLLRMLHS
QLNATIELVLVPADEYTEFPLQRFYRYLFDKKSSLGATSVEFRKLPVQPILTMKIDTPEA
WNVQAFHAGDDLDNLRVDPDSPAAVKATTSAVFQLESLLVYGQCRDTTFNMYAPPNGLQL
VLEREVGAQLLHRDTLVMKNLGYFQLQATPGVWSLHLARGRAAELFDIIDLETELPLETN
PITVYDFGSHISQLYVRKKEGKEHEELLQSVEHAAPDKPKSEADETSSTKSYWDAMLKMM
GKHDTKTQDTVEADQVDNDSAVVAQKQRTGETIHVFSVASGYLYERFVKIMMSSVLKRTN
NPVTFWLLENFLSPDFKKSIPVLREQFGMDIRLVTYKWPNWLRQQTEKQRIIWGYKILFL
DVLFPLGVQKIIYVDADQVVRADLKELWELDLDGKPYGYTPFCDSRNVGFQFWRQGYWKD
HLRGKPYHISALYVVDLALFRQMAAGDMLRAVYSHLSADPNSLANLDQDLPNYAQHQIPI
FSLPQEWLWCESWCSDETKVAAKTIDLCNNPKHKEPKLEMAKRVIAGELFNESWIELDQE
IKEAEAEYADQA

Enzyme Prediction     

EC	2.4.1.-:29

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT24	1353	1598	4e-122	0.9959677419354839

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
408203	Glyco_transf_24	0.0	1353	1623	1	267	Glucosyltransferase 24. This is the catalytic domain found in UDP-glucose:glycoprotein glucosyltransferase (UGGT). This domain belongs to glucosyltransferase 24 family (GT24) A-type domain. The GT domain displays the expected glycosyltransferase type A (GT-A) fold.
133054	GT8_HUGT1_C_like	9.96e-161	1353	1598	1	248	The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity.
132996	Glyco_transf_8	2.12e-58	1353	1593	1	243	Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+.
408199	Thioredoxin_12	4.86e-32	38	232	1	172	Thioredoxin-like domain. This is one of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).
399437	UDP-g_GGTase	1.57e-27	1182	1289	2	109	UDP-glucose:Glycoprotein Glucosyltransferase. UDP-g_GGTase is an important, central component of the QC system in the ER for checking that glycoproteins are folded correctly. This QC prevents incorrectly folded glycoproteins from leaving the ER.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UIZ20865.1|GT24	0.0	1	1632	1	1664
CCA14500.1|GT24	0.0	25	1628	35	1665
AIG56125.1|GT24	0.0	11	1628	11	1505
AIG55512.1|GT24	0.0	6	1629	12	1516
CAB4116261.1|GT24	1.79e-197	26	1622	34	1553

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MU1_A	2.82e-148	22	1623	5	1446	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5MZO_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TRF_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5NV4_A	3.88e-148	22	1623	5	1446	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TRT_A	1.01e-147	22	1623	5	1446	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS2_A	1.41e-141	961	1623	550	1212	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_C Chain C, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_D Chain D, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS8_A	6.52e-122	28	1560	4	1382	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS8_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q0WL80|UGGG_ARATH	8.86e-184	26	1622	31	1566	UDP-glucose:glycoprotein glucosyltransferase OS=Arabidopsis thaliana OX=3702 GN=UGGT PE=1 SV=1
sp|Q6P5E4|UGGG1_MOUSE	9.25e-184	2	1623	16	1522	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Mus musculus OX=10090 GN=Uggt1 PE=1 SV=4
sp|Q9NYU2|UGGG1_HUMAN	5.17e-183	25	1628	44	1527	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Homo sapiens OX=9606 GN=UGGT1 PE=1 SV=3
sp|Q9JLA3|UGGG1_RAT	2.39e-180	24	1623	43	1522	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Rattus norvegicus OX=10116 GN=Uggt1 PE=1 SV=2
sp|Q9NYU1|UGGG2_HUMAN	5.88e-179	21	1623	28	1497	UDP-glucose:glycoprotein glucosyltransferase 2 OS=Homo sapiens OX=9606 GN=UGGT2 PE=1 SV=4

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000265	0.999713	CS pos: 25-26. Pr: 0.9523

TMHMM  Annotations     

There is no transmembrane helices in PITG_07201-t26_1-p1.