CAZyme Information: PHYSODRAFT_497355-t26_1-p1

Basic Information

• Species: Phytophthora sojae
• Lineage: Oomycota; NA; ; Peronosporaceae; Phytophthora; Phytophthora sojae
• CAZyme ID: PHYSODRAFT_497355-t26_1-p1
• CAZy Family: GH81
• CAZyme Description: glycosyl hydrolase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
750		81773.10	4.4728

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PsojaeP6497	28142	1094619	1653	26489

• Gene Location: location=JH159153:10328191-10330530(+)

Full Sequence      

MLMSWFSAVCCLAMGLAAHQSAPLVSADQWDAQTDVIIESMDEDQLVGQMVQVAFSRFFN
ASKTFDEDKFRAYAKLKIGSYLSGPWQSGSYNGKYAWTAQEWRDVLSRAQEIVKEENDGH
PFIFGIDSLHGAGFTSGAVLFGQPINGAATFNPDLIYDMGRIAGRDTLAAGTPWIFGPML
EISQNPLWPRVYETFGEDPYLVSVIAESLIKGIQSNNGSAACMKHWIGYSKTPTGHDKDG
VTLSDYDLLNYFLPPFKAAVDAGVLSAMENYISVNGIPTIANPKLMNKLLREDLGFEGMT
RTSIDMTMASSDYGFINATKALIANDLSFLNRVKESARRVVKLKLQLGLYDNAVPGADLV
DQVGNDEDKAAALNSARESIVLLQNNDSVLPIPETAKVFLTGHSADNVGYQCGGWSLSQQ
GYSGNDMFPNGISVKDGFQAIANETVTYFNGLNYTGTYNESDLAHAKELASQAEYTIAVI
GEHSYTEKTYGDINDLALPAGQIEYVTELASTGTKVIAVLVGGRPRLLGELPSNVHAVVN
AMLPCELGGQAIAEIIYGRVNPSGRMPITYPKDAGNILMPYNHRVSTQCASGDYCEMQWE
FGHGLSYTEFTYSDMTLSRTNVTSVTETVDVSVVVKNSGSVAGKETVMLFLTQPFRTISV
PEVKQLKKFTKVELQAGESTTVNFTLSTDDWSVYYPQIGNGLKKVAEDADYVIAIKPETD
CDVYNETAVANPLCATFTLQTGEHPYGSFE

Enzyme Prediction     

EC	3.2.1.21:1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	115	305	6.5e-55	0.8703703703703703

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
185053	PRK15098	2.80e-100	3	715	1	750	beta-glucosidase BglX.
224389	BglX	2.34e-59	44	425	4	381	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
178629	PLN03080	1.39e-52	121	686	83	742	Probable beta-xylosidase; Provisional
396478	Glyco_hydro_3_C	1.99e-45	380	607	1	216	Glycosyl hydrolase family 3 C-terminal domain. This domain is involved in catalysis and may be involved in binding beta-glucan. This domain is found associated with pfam00933.
395747	Glyco_hydro_3	2.66e-44	44	354	3	310	Glycosyl hydrolase family 3 N terminal domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UIZ29009.1|GH3	7.62e-295	1	739	1	762
UIZ29613.1|GH3	9.59e-268	17	745	53	825
CCA27737.1|GH3	2.66e-266	32	739	66	808
UIZ29432.1|GH3	3.45e-264	17	745	8	769
UIZ27116.1|GH3	6.10e-264	50	740	1	725

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5JP0_A	5.70e-104	38	690	21	718	Bacteroides ovatus Xyloglucan PUL GH3B with bound glucose [Bacteroides ovatus],5JP0_B Bacteroides ovatus Xyloglucan PUL GH3B with bound glucose [Bacteroides ovatus]
5XXN_A	3.26e-76	26	715	6	737	Crystal Structure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose [Bacteroides thetaiotaomicron VPI-5482],5XXN_B Crystal Structure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose [Bacteroides thetaiotaomicron VPI-5482],5XXO_A Crystal structure of mutant (D286N) GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorotriose [Bacteroides thetaiotaomicron VPI-5482],5XXO_B Crystal structure of mutant (D286N) GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorotriose [Bacteroides thetaiotaomicron VPI-5482]
5XXL_A	4.52e-76	26	715	6	737	Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXL_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXM_A Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482],5XXM_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482]
7EAP_A	5.91e-72	104	693	79	726	Chain A, Fn3_like domain-containing protein [Aspergillus oryzae RIB40]
5YOT_A	8.15e-72	104	693	79	726	Isoprimeverose-producing enzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40],5YOT_B Isoprimeverose-producing enzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40],5YQS_A Isoprimeverose-producing enzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40],5YQS_B Isoprimeverose-producing enzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q23892|GLUA_DICDI	1.11e-118	31	715	77	811	Lysosomal beta glucosidase OS=Dictyostelium discoideum OX=44689 GN=gluA PE=1 SV=2
sp|A7LXU3|BGH3B_BACO1	4.05e-103	38	690	43	740	Beta-glucosidase BoGH3B OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02659 PE=1 SV=1
sp|Q56078|BGLX_SALTY	1.29e-67	103	689	87	728	Periplasmic beta-glucosidase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=bglX PE=3 SV=2
sp|T2KMH0|PLH24_FORAG	3.23e-67	105	722	50	713	Beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22130 PE=1 SV=1
sp|P33363|BGLX_ECOLI	1.13e-65	103	689	87	728	Periplasmic beta-glucosidase OS=Escherichia coli (strain K12) OX=83333 GN=bglX PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000230	0.999773	CS pos: 27-28. Pr: 0.8135

TMHMM  Annotations     

There is no transmembrane helices in PHYSODRAFT_497355-t26_1-p1.