CAZyme Information: PHYSODRAFT_495971-t26_1-p1

Basic Information

• Species: Phytophthora sojae
• Lineage: Oomycota; NA; ; Peronosporaceae; Phytophthora; Phytophthora sojae
• CAZyme ID: PHYSODRAFT_495971-t26_1-p1
• CAZy Family: GH81
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
692		79677.22	5.2057

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PsojaeP6497	28142	1094619	1653	26489

• Gene Location: location=JH159153:7465159-7467321(-)

Full Sequence      

MAYGLQWYLKTALHTQTDWDDHKLQLPYVLPKVDKRVRHKRSAKFSYYQNVCTVSYSSWA
WGWQKWEKHIDWMALNGINMPLAFTGQEKVWQNTFKKHYNVSSAGLNKFFAGAAFLAWGR
MGNLRGSWVEGPLPQAFIDGQYELQLKILERMRGFGMVPALPAFAGHVPEELKTLYPNAK
FTRSPNWGGFSDEFCCVYMLDPQDPLYYEIGKTFLEEQRALYDYTSSLYQCDTYNEMDPD
FTDPAKLQAASRAVIDSMTAADPNAVWLIQGWLFVNSPNYWTKERVQTYLDGVPNDKMII
LDLYSEVRPVWNKMDNYFGKSWIYCVLHNFGGNTGMRGDLPTLGTAPVLANRASSGTMIG
MGLTMEGIFQNYVVYDLTLQMAWVDAPLDMDEWVPSFAAQRYHSQDAHTERAWGFLLQSV
YNRTLGYGGVTKSLVCLIPHWKLVRDGFMPTLITYDPMDITRAWKELLLAGSELHAVDTY
RHDLVDVTRQFLSDHFMAQYLHLEDMYAGKETPADQLCAWTDRMLVTIEWLDEILATNDD
FLLGNWVADARALADEVGAAEVTSLQDYYEYEARNQVTRWGDNNSESIHDYAGKEWAGLV
SGYYLPRWRMWLTEVCQSYTQKRDVNEAALKKARVDFELNWQLSHERYPTTTTGDTLAVS
KRIYEEFAGVSSFQAPWSWRMTGYLAGNQVVA

Enzyme Prediction     

EC	3.2.1.50:13

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH89	1	665	2.6e-218	0.975867269984917

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
398659	NAGLU	0.0	47	383	2	331	Alpha-N-acetylglucosaminidase (NAGLU) tim-barrel domain. Alpha-N-acetylglucosaminidase, a lysosomal enzyme required for the stepwise degradation of heparan sulfate. Mutations on the alpha-N-acetylglucosaminidase (NAGLU) gene can lead to Mucopolysaccharidosis type IIIB (MPS IIIB; or Sanfilippo syndrome type B) characterized by neurological dysfunction but relatively mild somatic manifestations. The structure shows that the enzyme is composed of three domains. This central domain has a tim barrel fold.
404009	NAGLU_C	3.26e-94	393	661	1	258	Alpha-N-acetylglucosaminidase (NAGLU) C-terminal domain. Alpha-N-acetylglucosaminidase, a lysosomal enzyme required for the stepwise degradation of heparan sulfate. Mutations on the alpha-N-acetylglucosaminidase (NAGLU) gene can lead to Mucopolysaccharidosis type IIIB (MPS IIIB; or Sanfilippo syndrome type B) characterized by neurological dysfunction but relatively mild somatic manifestations. The structure shows that the enzyme is composed of three domains. This C-terminal domain has an all alpha helical fold.
404008	NAGLU_N	1.99e-06	1	31	51	81	Alpha-N-acetylglucosaminidase (NAGLU) N-terminal domain. Alpha-N-acetylglucosaminidase, a lysosomal enzyme required for the stepwise degradation of heparan sulfate. Mutations on the alpha-N-acetylglucosaminidase (NAGLU) gene can lead to Mucopolysaccharidosis type IIIB (MPS IIIB; or Sanfilippo syndrome type B) characterized by neurological dysfunction but relatively mild somatic manifestations. The structure shows that the enzyme is composed of three domains. This N-terminal domain has an alpha-beta fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AIG56008.1|GH89	5.29e-312	1	676	81	747
AIG55486.1|GH89	3.24e-310	1	688	78	746
AIG56414.1|GH89	7.41e-244	1	669	88	750
AIG56322.1|GH89	7.24e-227	1	667	86	747
AIG55749.1|GH89	8.93e-223	1	667	84	748

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4XWH_A	6.94e-162	2	667	63	711	Crystal structure of the human N-acetyl-alpha-glucosaminidase [Homo sapiens]
2VC9_A	4.93e-80	1	672	224	881	Family 89 Glycoside Hydrolase from Clostridium perfringens in complex with 2-acetamido-1,2-dideoxynojirmycin [Clostridium perfringens],2VCA_A Family 89 glycoside hydrolase from Clostridium perfringens in complex with beta-N-acetyl-D-glucosamine [Clostridium perfringens],2VCB_A Family 89 Glycoside Hydrolase from Clostridium perfringens in complex with PUGNAc [Clostridium perfringens],2VCC_A Family 89 Glycoside Hydrolase from Clostridium perfringens [Clostridium perfringens]
7MFK_A	5.56e-80	1	672	232	889	Chain A, Alpha-N-acetylglucosaminidase family protein [Clostridium perfringens ATCC 13124],7MFL_A Chain A, Alpha-N-acetylglucosaminidase family protein [Clostridium perfringens ATCC 13124]
4A4A_A	4.83e-79	1	672	247	904	CpGH89 (E483Q, E601Q), from Clostridium perfringens, in complex with its substrate GlcNAc-alpha-1,4-galactose [Clostridium perfringens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q9FNA3|NAGLU_ARATH	1.23e-172	1	667	105	804	Alpha-N-acetylglucosaminidase OS=Arabidopsis thaliana OX=3702 GN=NAGLU PE=2 SV=1
sp|P54802|ANAG_HUMAN	7.13e-161	2	667	86	734	Alpha-N-acetylglucosaminidase OS=Homo sapiens OX=9606 GN=NAGLU PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000027	0.000000

TMHMM  Annotations     

There is no transmembrane helices in PHYSODRAFT_495971-t26_1-p1.