CAZyme Information: PHYSODRAFT_249236-t26_1-p1

Basic Information

• Species: Phytophthora sojae
• Lineage: Oomycota; NA; ; Peronosporaceae; Phytophthora; Phytophthora sojae
• CAZyme ID: PHYSODRAFT_249236-t26_1-p1
• CAZy Family: AA17
• CAZyme Description: putative endo-1,3-beta-glucanase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
825		88568.89	5.2281

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PsojaeP6497	28142	1094619	1653	26489

• Gene Location: location=JH159164:958273-960846(-)

Full Sequence      

MAIASAAAFDLSLLDDDDILREGTVYAPKQEEGRAFGASLSHSSSGSAGSFSASTSFSTS
GSGSSFSASGSGSSFSASGSGSSTSISGSGASFSASGSDASLSTSGSDASSSTSSDSSLS
ASGSDAASLSASGSGPGSAAPPTTSSTGSADTTPTSGKGGLLAPFSTEEPPETLFPRNVL
GEMAPIINVAKGLLGKPLPTNKWWGNLIHTTVEGLNTKANPGWSNPYAVKLPKEAPYGIQ
ACYSYNYRQLSALTDGVAEFYLHDFVNDITLSATEFAGEANPIYEVYSFSDFGIEVRACL
ENEQQCIDSALVHGMAFITATYDSLTASIESEYTMKIVDKSVPGKYIIDLGGNQTFAIDE
SRKALVSSKPFTGTVRVAILPSSEATDVYDKYRTCHVRGGNVSVASRTEYSLQWETVGKS
CKTHGLLHFALPHHLPALKGAITEKDPKAIVLNSATRVDAEDDLEVDFYPTAKPSAKKVS
KLGLLKTLEADIADEWSLNKTSWYFNGKQYQKYASLCLLAGDEAVVGKDKKLLQTCLSKL
QTLLEPFLDNTLAPPLNYDTSYSGLVSSLGFTLNNVDADFGNTVYNDHHYHYGYWVTASA
MLKSLDPKWKRMKELDAMIWMMLRDVANPSAEDAFFPKFRYFSFYFGHSYSHGVTPMADG
KDEESTSEDVNFYYGMKLWGQVSKNKAVEDLGSLMLRLNARAIRTYFLMTSDNTIHPKEF
VPNHVTGIFFDNKAAYATWFSAERYAIHGIQMIPVSPINALEAIVTQANTTNAWLSLLLV
NEAAVDQADALPKLKQATMDDGLTRSWALYNAAARSHSAKKPTPA

Enzyme Prediction     

No EC number prediction in PHYSODRAFT_249236-t26_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH81	191	810	1.8e-150	0.977491961414791

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
407548	Glyco_hydro81C	5.10e-128	484	809	14	349	Glycosyl hydrolase family 81 C-terminal domain. Family of eukaryotic beta-1,3-glucanases. Within the Aspergillus fumigatus protein, two perfectly conserved Glu residues (E550 or E554) have been proposed as putative nucleophiles of the active site of the Engl1 endoglucanase, while the proton donor would be D475. The endo-beta-1,3-glucanase activity is essential for efficient spore release. This entry represents the helical C-terminal domain.
227785	Acf2	4.54e-116	137	814	37	754	Endoglucanase Acf2 [Carbohydrate transport and metabolism].
397619	Glyco_hydro_81	3.51e-37	195	457	12	292	Glycosyl hydrolase family 81. Family of eukaryotic beta-1,3-glucanases. Within the Aspergillus fumigatus protein ENGL1, two perfectly conserved Glu residues (E550 or E554) have been proposed as putative nucleophiles of the active site of the Engl1 endoglucanase, while the proton donor would be D475. The endo-beta-1,3-glucanase activity is essential for efficient spore release.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UIZ26438.1|GH81	4.99e-311	159	815	42	740
UIZ26440.1|GH81	4.99e-311	159	815	42	740
UIZ26131.1|GH81	9.29e-254	160	815	11	714
UIZ26137.1|GH81	1.11e-251	142	815	103	824
UIZ26602.1|GH81	1.11e-251	142	815	103	824

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4K3A_A	1.07e-82	155	817	2	720	The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei],4K3A_B The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei]
5XBZ_A	1.72e-82	155	817	27	745	Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminaripentaose [Rhizomucor miehei],5XBZ_B Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminaripentaose [Rhizomucor miehei],5XC2_A Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei],5XC2_B Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei],5XC2_C Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei],5XC2_D Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei]
4K35_A	2.60e-79	195	817	38	720	The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei],4K35_B The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei]
6FOP_A	4.33e-24	586	756	414	582	Chain A, Glycoside hydrolase family 81 [Acetivibrio thermocellus ATCC 27405]
5V1W_A	1.43e-23	197	803	48	667	Chain A, Glycoside Hydrolase [Halalkalibacterium halodurans C-125]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q09850|ENG2_SCHPO	3.55e-77	161	818	3	705	Ascus wall endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng2 PE=1 SV=1
sp|D4AZ24|ENG1_ARTBC	3.46e-73	144	813	163	900	Probable endo-1,3(4)-beta-glucanase ARB_01444 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_01444 PE=1 SV=1
sp|Q9UT45|ENG1_SCHPO	1.21e-72	172	813	53	735	Primary septum endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng1 PE=3 SV=1
sp|Q12168|ENG2_YEAST	4.70e-69	197	816	110	779	Endo-1,3(4)-beta-glucanase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ACF2 PE=1 SV=1
sp|Q5AIR7|ENG1_CANAL	4.23e-66	159	813	426	1139	Endo-1,3(4)-beta-glucanase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=ENG1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.999956	0.000074

TMHMM  Annotations     

There is no transmembrane helices in PHYSODRAFT_249236-t26_1-p1.