CAZyme Information: PHYBL_176989T0-p1

Basic Information

• Species: Phycomyces blakesleeanus
• Lineage: Mucoromycota; Mucoromycetes; ; Phycomycetaceae; Phycomyces; Phycomyces blakesleeanus
• CAZyme ID: PHYBL_176989T0-p1
• CAZy Family: GH5
• CAZyme Description: Mannosyltransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
438	PblaNRRL1555-_SC04|CGC2	50486.18	9.6287

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PblakesleeanusNRRL1555	16528	763407	0	16528

• Gene Location: location=PblaNRRL1555-_SC04:1805227-1807502(+)

Full Sequence      

MAQSVKRKRPRQSTINSNDSKVIKLEDIGKFLTNVLMDPSYIWYIATILLLGELVLNIVI
IQKVAYTEIDWTAYMQEVKGFIEGERDYLNLRGDTGPLVYPAGFVYIYSGLYYLTNKGTN
VRLAQYIFAGLYLATQAVVFALYSKSKKFPPYALALLCLSKRLHSIYALRCFNDPVAMFF
MYSCILAMIHKRWVFSGILYSLAISVKMNVLLFFPAFGILLWQAIGAWRTFAVLSLMGSI
QIVLGYPFLSTYPESYLGRAFEFSRSFDYQWTVNWRMVTEKTFGSGVFAKSLLAGHAIVL
FLFLYFVWCKRGGGLVKVFLDGFKGNSVRRSVSPDDILSMMFTSNLIGMVFARSLHYQFY
SWYYHALPYLLWQSVWMTTGSQTFRNYIRIFVLATIEACWMTFPSTERSSWTLFACHAIL
LTGLFKRDQEETEQSKNE

Enzyme Prediction     

EC	2.4.1.258:18

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT58	47	410	1e-139	0.9945054945054945

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
398745	ALG3	0.0	47	410	1	358	ALG3 protein. The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl pyrophosphate. Whereas early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions from Man5GlcNAc2-PP-Dol to Man9GlcNAc2-PP-Dol on the lumenal side use Dol-P-Man. ALG3 gene encodes the Dol-P-Man:Man5GlcNAc2-PP-Dol mannosyltransferase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CDS08832.1|GT58	2.67e-187	31	425	10	397
SVE87338.1|GT58	5.87e-118	1	427	1	415
SVE87964.1|GT58	5.87e-118	1	427	1	415
SVE86082.1|GT58	5.87e-118	1	427	1	415
SVE86711.1|GT58	5.87e-118	1	427	1	415

PDB Hits     

PHYBL_176989T0-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q92685|ALG3_HUMAN	3.24e-111	36	436	36	435	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Homo sapiens OX=9606 GN=ALG3 PE=1 SV=1
sp|Q8K2A8|ALG3_MOUSE	4.15e-109	36	425	36	421	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Mus musculus OX=10090 GN=Alg3 PE=1 SV=2
sp|Q27333|NT56_DROME	3.64e-102	30	427	33	487	Lethal(2)neighbour of tid protein OS=Drosophila melanogaster OX=7227 GN=l(2)not PE=1 SV=1
sp|Q24332|NT56_DROVI	5.05e-100	31	424	33	489	Lethal(2)neighbour of Tid protein OS=Drosophila virilis OX=7244 GN=l(2)not PE=1 SV=1
sp|O82244|ALG3_ARATH	8.60e-100	49	436	39	427	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Arabidopsis thaliana OX=3702 GN=ALG3 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000007	0.000029

TMHMM  Annotations     

Start	End
40	62
97	114
121	143
166	188
195	217
227	249
286	308