CAZyme Information: PHPALM_1066-t46_1-p1

Basic Information

• Species: Phytophthora palmivora
• Lineage: Oomycota; NA; ; Peronosporaceae; Phytophthora; Phytophthora palmivora
• CAZyme ID: PHPALM_1066-t46_1-p1
• CAZy Family: AA17
• CAZyme Description: Beta-glucan synthesis-associated protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
654	NCKW01000192|CGC1	71120.26	3.9437

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Ppalmivorasbr112.9	28185	N/A	3511	24674

• Gene Location: location=NCKW01000192:5185-7149(-)

Full Sequence      

MDKADGVNSALEIYMHNMTGTECDNDDNCYFYIEADTGETTLTLWNDYLSPAGYEDVTFY
YKAGMVQSWNKFCFQGGMVEVRAQLPGAVTNASGNPDVETGSGSVRASNIDYYPTWPGIW
LMGNLGRAIFSASTARMWPFSYNECNETIFDSQNQRISACDDDPGHGMNANQGRGAPEID
ILEGGGTAISSSIQVGPGMPENFRVIPDAESSYCYYTYDCTTEGANNQDVPTAYYESLRG
HKSWYQGLRYGANNFCDTDSTLVQSYKTIAASLDKGITENACTMELCPASYDVNADLGYI
DNGTDHWGVNTNGTCFPKQNAYTGAFLCSAGNNDTSCAASSGSTDAMSEFAYQMDAISSN
WEIQVAAYTDWLTYQLEWVMGDEGYIRWMLGGKPLFEITADVLTNPPQDAAQMNPKKIML
EEPMYVIFNVALSSSWGSSPPNAGNGGCYGDGSDATANAICDSFPMYLKIDYIRVYQDKS
DGSNMAVGCDPSTHPTKQWIEDNIDSYVDDDNPWTEVSGKAFCDSSEDCTIETNSSSTIT
TGTCVNGRCECSATTWTGPRCTVATSSSDSSSDGFFSSNSYGPPMYVSGVFMAVTVLTTF
VSVYLSILAARKSDELLQKEIMAKKGAQSSSSPGAFMGESMMKPPKENYSTNFV

Enzyme Prediction     

EC	-	-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	2	206	1.4e-50	0.5429447852760736

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397841	SKN1	2.70e-44	60	507	181	476	Beta-glucan synthesis-associated protein (SKN1). This family consists of the beta-glucan synthesis-associated proteins KRE6 and SKN1. Beta1,6-Glucan is a key component of the yeast cell wall, interconnecting cell wall proteins, beta1,3-glucan, and chitin. It has been postulated that the synthesis of beta1,6-glucan begins in the endoplasmic reticulum with the formation of protein-bound primer structures and that these primer structures are extended in the Golgi complex by two putative glucosyltransferases that are functionally redundant, Kre6 and Skn1. This is followed by maturation steps at the cell surface and by coupling to other cell wall macromolecules.
185689	GH16_fungal_KRE6_glucanase	1.56e-40	54	477	57	295	Saccharomyces cerevisiae KRE6 and related glucanses, member of glycosyl hydrolase family 16. KRE6 is a Saccharomyces cerevisiae glucanase that participates in the synthesis of beta-1,6-glucan, a major structural component of the cell wall. It is a golgi membrane protein required for normal beta-1,6-glucan levels in the cell wall. KRE6 is closely realted to laminarinase, a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
185693	GH16_laminarinase_like	3.23e-12	356	476	145	235	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
225182	BglS	2.98e-05	60	145	102	156	Beta-glucanase, GH16 family [Carbohydrate transport and metabolism].
185683	Glyco_hydrolase_16	1.73e-04	369	476	142	210	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UIZ27996.1|GH16	2.37e-317	1	588	85	666
UIZ28151.1|GH16	1.24e-262	1	654	85	752
UIZ28149.1|GH16	2.50e-262	1	654	84	754
UIZ27810.1|GH16	1.15e-254	1	629	120	758
UIZ28152.1|GH16	1.37e-245	1	654	73	739

PDB Hits     

PHPALM_1066-t46_1-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P33336|SKN1_YEAST	1.02e-23	37	522	424	754	Beta-glucan synthesis-associated protein SKN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SKN1 PE=1 SV=1
sp|P32486|KRE6_YEAST	6.68e-23	60	522	390	702	Beta-glucan synthesis-associated protein KRE6 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=KRE6 PE=1 SV=2
sp|O13941|YEPB_SCHPO	2.09e-21	61	519	303	615	Uncharacterized beta-glucan synthesis-associated protein C23H3.11c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC23H3.11c PE=3 SV=1
sp|P87023|KRE6_CANAX	2.66e-21	37	507	397	717	Beta-glucan synthesis-associated protein KRE6 OS=Candida albicans OX=5476 GN=KRE6 PE=2 SV=1
sp|O13789|YEOB_SCHPO	3.75e-21	30	517	279	623	Uncharacterized beta-glucan synthesis-associated protein C17G6.11c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC17G6.11c PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000053	0.000010

TMHMM  Annotations     

Start	End
586	608