CAZyme Information: PGTG_20839-t26_2-p1

Basic Information

• Species: Puccinia graminis
• Lineage: Basidiomycota; Pucciniomycetes; ; Pucciniaceae; Puccinia; Puccinia graminis
• CAZyme ID: PGTG_20839-t26_2-p1
• CAZy Family: GT57
• CAZyme Description: chitin synthase, variant

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
743		83240.27	7.3552

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PgraminisCRL75-36-700-3	16309	418459	509	15800

• Gene Location: location=DS178265:162523-166596(-)

Full Sequence      

MSYADPFSDPHYQHDYYDGAVNPREHEPVTSYGEEDSVDEEFLEKDYHQVGITGQEYPPE
PVSATSFGFPAPQLPADTSVGGREATARLYRTQTSAEAWSKRQKVNRTRAKTIKVKLSKG
NFVHEYPVPTPVKHANLMGGHIGKDNEFTHMRYTAVTCDPDDFTREAGWSLRPQKYGRDI
ELLVAITYYNEDKVLVARTLHGVMLNLKEICKAHWSEFKKMAEKGAPETGTGAAWKKIAV
VLVFDGIEPADKATLDLLATLGVYQDGVMKKEVDGKETQAHVFEYTTMLSVTPKLELISP
QENDADNLVPVQMTFVLKQKNAKKINSHRWLFNAICPHLEPEVCILLDAGTKPGPRSLYY
LWEAFFHNPNLGGACGEIHAMLSGGKKLLNPLVAAQNFEYKMSNILDKPLESSFGYVSVL
PGAFSAYRTRAIRGRPLQQYFHGDHSLAAKLGDKGVQGMGIFKKNMFLAEDRILCFELVA
KAKDKWVLGYVKPAKGETDVPESAAELIGQRRRWLNGSFAAGVYALAHFPRMYKTWHNPI
RLLFLHVQAIYTTVGLIMSWFSLANWYVTFAAIIDLVHQFWRDPYAAGGFQDNRNTTDPF
KSPGGTSAEKIAWVTMECWSGTVLDNTAHFNNPDSVPDKYDWLGQLNYALKLVYLALVSL
QIILALGNRPKGEKKAYLISLCIFASFAIYLLTMTLFLTVKAFCPIHVQLDEAEASGTSQ
ASVFMSTSYGTIGFYILHTHAFI

Enzyme Prediction     

EC	2.4.1.16:26	2.4.1.16:26

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT2	188	365	1.5e-66	0.9938650306748467

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
133033	Chitin_synth_C	7.70e-92	184	521	1	244	C-terminal domain of Chitin Synthase catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin. Chitin synthase, also called UDP-N-acetyl-D-glucosamine:chitin 4-beta-N-acetylglucosaminyltransferase, catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin, which is a linear homopolymer of GlcNAc residues formed by covalent beta-1,4 linkages. Chitin is an important component of the cell wall of fungi and bacteria and it is synthesized on the cytoplasmic surface of the cell membrane by membrane bound chitin synthases. Studies with fungi have revealed that most of them contain more than one chitin synthase gene. At least five subclasses of chitin synthases have been identified.
396286	Chitin_synth_1	2.15e-91	188	365	1	163	Chitin synthase. This region is found commonly in chitin synthases classes I, II and III. Chitin a linear homopolymer of GlcNAc residues, it is an important component of the cell wall of fungi and is synthesized on the cytoplasmic surface of the cell membrane by membrane bound chitin synthases.
400626	Chitin_synth_1N	2.72e-33	114	187	1	70	Chitin synthase N-terminal. This is the N-terminal domain of Chitin synthase (pfam01644).
224136	BcsA	1.16e-26	316	587	117	351	Glycosyltransferase, catalytic subunit of cellulose synthase and poly-beta-1,6-N-acetylglucosamine synthase [Cell motility].
133045	CESA_like	7.03e-19	308	433	50	167	CESA_like is the cellulose synthase superfamily. The cellulose synthase (CESA) superfamily includes a wide variety of glycosyltransferase family 2 enzymes that share the common characteristic of catalyzing the elongation of polysaccharide chains. The members include cellulose synthase catalytic subunit, chitin synthase, glucan biosynthesis protein and other families of CESA-like proteins. Cellulose synthase catalyzes the polymerization reaction of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues in plants, most algae, some bacteria and fungi, and even some animals. In bacteria, algae and lower eukaryotes, there is a second unrelated type of cellulose synthase (Type II), which produces acylated cellulose, a derivative of cellulose. Chitin synthase catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin, which is a linear homopolymer of beta-(1,4)-linked GlcNAc residues and Glucan Biosynthesis protein catalyzes the elongation of beta-1,2 polyglucose chains of Glucan.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ABB70409.1|GT2	0.0	51	732	101	781
CDR40400.1|GT2	1.34e-280	51	732	115	766
CDI55582.1|GT2	1.15e-247	68	737	103	715
UOP57015.1|GT2	3.38e-247	69	737	101	716
UOP56885.1|GT2	3.38e-247	69	737	101	716

PDB Hits     

PGTG_20839-t26_2-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P30598|CHS1_USTMA	2.21e-245	74	737	116	727	Chitin synthase 1 OS=Ustilago maydis (strain 521 / FGSC 9021) OX=237631 GN=CHS1 PE=3 SV=3
sp|J9VMX7|CHS7_CRYNH	4.72e-231	61	737	88	710	Chitin synthase 7 OS=Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) OX=235443 GN=CHS7 PE=2 SV=2
sp|P30602|CHS3_EXODE	4.00e-229	95	732	115	708	Chitin synthase 3 OS=Exophiala dermatitidis OX=5970 GN=CHS3 PE=3 SV=2
sp|Q00757|CHSB_EMENI	1.71e-227	80	737	109	730	Chitin synthase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=chsB PE=2 SV=2
sp|P54267|CHSG_ASPFU	3.74e-225	3	732	45	712	Chitin synthase G OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=chsG PE=2 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000037	0.000009

TMHMM  Annotations     

Start	End
513	530
542	564
644	666
678	700
715	737