CAZyme Information: PGTG_17327-t26_1-p1

Basic Information

• Species: Puccinia graminis
• Lineage: Basidiomycota; Pucciniomycetes; ; Pucciniaceae; Puccinia; Puccinia graminis
• CAZyme ID: PGTG_17327-t26_1-p1
• CAZy Family: GT2
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
549		61513.74	5.1450

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PgraminisCRL75-36-700-3	16309	418459	509	15800

• Gene Location: location=DS178340:271826-274975(+)

Full Sequence      

MSSPYSQEDQFYAEVPVRRNRPSSTSSYSPYFMSHQSPGGSTEPSFGQGRYDWVHNGTQN
SRNPGSHLSKASSDKIADNYSSETPENAARYSYGAADLGFLTEELLSPGPADEMHAVEKK
TPRFGASRRPYDINWRFGYVPTIPGFRELIDPDTPREARSRVGADGEEYELVFSDEFNVD
GRTFWKGDDPFWEAADQHYWPTGDREWYDPDGAKTEGGYLVIRMNKMDAGINHNLDYRSG
MLTSWNKLCFTGGYMETGVIMPGKHDIGGFWPAIWTMGNLARPGFGATTDGTWPYSYDTC
DLGAMPNQTHPKTGEPGTTVLTSEVGDLSWQPGQKLSRCTCAGEDHPGPRMSDGTYKGRS
SPEIDMIEAYSYEKTGTAYGSQSLQIAPYDDKRTWNSKLGSTYQIYPESGFRTNVNPYHG
GNRQQTLSSLATLPPSNFELDGNQSMAYGLEWAPDQENSHITWSLAQKPTWTLNSTALQP
NSRSMVGQRLISNEPMYMILNLGLSDTFVPVNEAQLTFPAHMRFDYVRFYQRKGHKNVGC
SPKDYPTEK

Enzyme Prediction     

No EC number prediction in PGTG_17327-t26_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	175	530	2.6e-128	0.9969325153374233

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397841	SKN1	1.08e-178	140	549	87	467	Beta-glucan synthesis-associated protein (SKN1). This family consists of the beta-glucan synthesis-associated proteins KRE6 and SKN1. Beta1,6-Glucan is a key component of the yeast cell wall, interconnecting cell wall proteins, beta1,3-glucan, and chitin. It has been postulated that the synthesis of beta1,6-glucan begins in the endoplasmic reticulum with the formation of protein-bound primer structures and that these primer structures are extended in the Golgi complex by two putative glucosyltransferases that are functionally redundant, Kre6 and Skn1. This is followed by maturation steps at the cell surface and by coupling to other cell wall macromolecules.
185689	GH16_fungal_KRE6_glucanase	1.85e-136	171	531	1	295	Saccharomyces cerevisiae KRE6 and related glucanses, member of glycosyl hydrolase family 16. KRE6 is a Saccharomyces cerevisiae glucanase that participates in the synthesis of beta-1,6-glucan, a major structural component of the cell wall. It is a golgi membrane protein required for normal beta-1,6-glucan levels in the cell wall. KRE6 is closely realted to laminarinase, a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
225182	BglS	6.04e-38	148	544	28	355	Beta-glucanase, GH16 family [Carbohydrate transport and metabolism].
185693	GH16_laminarinase_like	1.41e-24	173	530	1	235	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
185683	Glyco_hydrolase_16	1.67e-05	448	530	147	210	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CCA67817.1|GH16_2	1.46e-139	138	548	198	603
QRW15086.1|GH16_2	7.57e-133	138	547	249	646
QRV86526.1|GH16_2	7.57e-133	138	547	249	646
QRW00570.1|GH16_2	1.04e-132	138	547	249	646
SAM82215.1|GH16_2	4.46e-131	138	547	205	612

PDB Hits     

PGTG_17327-t26_1-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P32486|KRE6_YEAST	1.13e-110	141	547	297	676	Beta-glucan synthesis-associated protein KRE6 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=KRE6 PE=1 SV=2
sp|P33336|SKN1_YEAST	8.46e-109	149	547	358	728	Beta-glucan synthesis-associated protein SKN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SKN1 PE=1 SV=1
sp|P87024|SKN1_CANAX	7.94e-108	131	547	317	703	Beta-glucan synthesis-associated protein SKN1 OS=Candida albicans OX=5476 GN=SKN1 PE=2 SV=1
sp|P87023|KRE6_CANAX	1.15e-103	131	547	313	706	Beta-glucan synthesis-associated protein KRE6 OS=Candida albicans OX=5476 GN=KRE6 PE=2 SV=1
sp|O13941|YEPB_SCHPO	1.80e-97	149	549	218	594	Uncharacterized beta-glucan synthesis-associated protein C23H3.11c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC23H3.11c PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000059	0.000000

TMHMM  Annotations     

There is no transmembrane helices in PGTG_17327-t26_1-p1.